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E.coli BioVectorTMLMG194 strain / Competent cells
Cat# BioVector931228
Classification Enterobacteriaceae, Escherichia
Strain Designations
BioVectorTM LMG194
Application
transformation host
Type Strain no
Biosafety Level 1
Genotype
F- delta(lacIPOZY)X74 galE galK thi rpsL deltaphoA ara714
Type Strain: no
Comments
The ara- phenotype results from a deletion of most of the araBAD operon.
Host strain (ara-) for use with the pBAD series of expression vectors.
Phenotype(s) confirmed: gal-, ara-, strR.
Lack of arabinose metabolism in this strain results in slower repression of expression for pBAD vectors, but also
gives a linear induction response (directly related to arabinose concentrations) when low concentrations of
inducer are used.
Medium: Nutrient agar or broth with 20 mcg/ml streptomycin
Growth Conditions
Temperature: 37.0°C
References
Guzman LM, et al. Tight regulation, modulation, and high-level expression by vectors containing the arabinose
PBAD promoter. J. Bacteriol. 177: 4121-4130, 1995. PubMed: 7608087
LMG194 E.coli strain， competent cells. 【Genotype】: F-, del(lac)X74, galE, thi, rpsL, del(phoA), del(ara)714,leu::Tn10
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