| 出品公司: | Agilent |
|---|---|
| 载体名称: | pCFB |
| 质粒类型: | 逆病毒载体 |
| 高拷贝/低拷贝: | 低拷贝 |
| 克隆方法: | 限制性内切酶，多克隆位点 |
| 启动子: | CMV |
| 载体大小: | 5292 bp |
| 5' 测序引物及序列: | CMV Forward： CGCAAATGGGCGGTAGGCGTG |
| 3' 测序引物及序列: | -- |
| 载体标签: | -- |
| 载体抗性: | 氨苄青霉素 |
| 筛选标记: | 无 |
| 克隆菌株: | DH5α 等通用菌株 |
| 宿主细胞（系）: | 常规细胞系（293、CV-1、CHO等） |
| 备注: | -- |
| 产品目录号: | -- |
| 稳定性: | 稳表达 |
| 组成型/诱导型: | 组成型 |
| 病毒/非病毒: | 逆转录病毒 |
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载体基本信息
载体质粒图谱和多克隆位点信息
载体简介
pCFB载体限制性酶切位点pCFB, 5292 bp version 013001Enzymes with 1-10 cleavage sites: #sites -- Bp position of recognition site -- AatII 6 328, 381, 464, 650, 1153 5218 Acc65I 3 822, 2021, 2818 AccI 3 2142, 2232, 3178 AflII 3 202, 1440, 2373 AflIII 2 164, 3407 AgeI 1 2015 AhdI 6 862, 908, 1449, 2858, 2904 4295 Alw44I 4 1229, 3223, 3721, 4967 AlwNI 3 2494, 2569, 3818 ApaI 1 1413 ApoI 3 87, 1303, 2238 AvaI 8 785, 818, 1416, 2193, 2249 2748, 2781, 2814 BamHI 1 2244 BanII 5 939, 1413, 2751, 2764, 2935 BbeI 3 790, 1831, 2786 BbsI 1 5285 BbvCI 1 2624 BciVI 5 831, 2161, 2827, 3616, 5143 BglI 4 291, 413, 484, 4414 BglII 1 1853 BpmI 3 1974, 2321, 4385 Bpu10I 4 1723, 2508, 2583, 2624 BsaAI 4 545, 1991, 2175, 3159 BsaHI 10 328, 381, 464, 650, 790 1153, 1831, 2786, 4836, 5218 BsaI 9 869, 890, 957, 1589, 1977 2865, 2886, 2953, 4367 BsaWI 5 138, 2015, 3613, 3760, 4591 BseRI 5 893, 1243, 1736, 1775, 2889 BsiEI 8 1136, 1209, 2013, 2255, 3320 3744, 4667, 4816 BsiHKAI 6 1229, 2751, 3223, 3721, 4882 4967 BsmBI 6 1151, 1268, 1512, 1571, 1757 3056 Bsp120I 1 1413 BspHI 3 4127, 5135, 5240 BspLU11I 2 164, 3407 BsrBI 5 1474, 2252, 2277, 3338, 5139 BsrDI 2 4354, 4536 BsrGI 1 1716 BssHII 1 2734 BssSI 3 3580, 4964, 5271 Bst1107I 1 3178 BstAPI 3 2493, 2568, 3225 BstEII 1 1521 BstF5I 10 777, 857, 1561, 1748, 2206 2853, 3082, 4280, 4461, 4748 Bsu36I 1 1451 Cfr10I 2 2015, 4380 DraI 3 4164, 4183, 4875 DraIII 1 2048 DrdI 2 3096, 3509 DsaI 3 151, 567, 1106 EaeI 7 1002, 1136, 1543, 1564, 1843 2255, 4688 EarI 4 1509, 1739, 3285, 5089 Ecl136II 1 2751 Eco52I 2 1136, 2255 Eco57I 2 3934, 4982 Eco72I 1 2175 EcoNI 1 1822 EcoO109I 4 1652, 2099, 2670, 5275 EcoRI 1 2238 EcoRV 2 2479, 2554 EheI 3 790, 1831, 2786 FokI 10 777, 857, 1561, 1748, 2206 2853, 3082, 4280, 4461, 4748 FspI 1 4520 HaeII 5 790, 1831, 2786, 3281, 3651 HgaI 7 724, 1064, 1341, 3113, 3509 4087, 4837 HincII 2 2232, 4839 KasI 3 790, 1831, 2786 KpnI 3 822, 2021, 2818 MboII 10 1509, 1740, 2271, 3286, 4057 4148, 4903, 4981, 5090, 5286 MscI 3 1002, 1543, 1843 MslI 5 159, 568, 4548, 4707, 5066 MspA1I 9 151, 918, 2457, 2532, 2914 3117, 3747, 3992, 4933 MunI 2 11, 21 NarI 3 790, 1831, 2786 NcoI 1 567 NdeI 4 440, 1839, 1847, 3229 NheI 5 6, 16, 26, 197, 2368 NotI 1 2254 NsiI 1 774 NspI 4 164, 244, 3042, 3407 PleI 10 617, 812, 1195, 1220, 2038 2283, 2808, 3307, 3778, 4295 Ppu10I 1 774 PpuMI 3 1652, 2099, 2670 PshAI 1 1190 Psp1406I 2 4525, 4898 PstI 3 1353, 1535, 4541 PvuI 2 1209, 4667 PvuII 2 2457, 2532 SacI 1 2751 SacII 1 151 SalI 1 2232 SapI 1 3284 ScaI 1 4778 SchI 10 617, 812, 1195, 1220, 2038 2283, 2808, 3307, 3778, 4295 SexAI 1 1649 SfcI 7 182, 1353, 1535, 2353, 3672 3863, 4541 SmaI 4 818, 1416, 2193, 2814 SmlI 8 202, 1440, 2249, 2373, 3513 3775, 4052, 4920 SnaBI 1 545 SpeI 1 1072 SphI 1 244 SrfI 1 1415 SspI 1 5102 StyI 5 567, 881, 1679, 2667, 2877 TaqI 9 1472, 1783, 1794, 2225, 2233 2250, 2294, 3507, 4951 TatI 7 424, 504, 537, 588, 1716 3213, 4778 TfiI 1 3382 Tsp45I 6 1457, 1666, 3062, 3157, 4557 4768 TspRI 10 1459, 2050, 3303, 3809, 3822 4093, 4242, 4347, 4694, 4721 Tth111I 4 808, 1648, 2804, 3151 VspI 1 4471 XbaI 2 2211, 2635 XhoI 1 2249 XhoII 8 1853, 2244, 4048, 4059, 4145 4157, 4925, 4942 XmaI 4 818, 1416, 2193, 2814 XmnI 1 4895Enzymes that do NOT cut molecule:AccIII AscI BclI BlnI Bpu1102I BsaBI BsgI BsiWI BsmI BspMI BstBI BstXI ClaI Eco47III FseI HindIII HpaI MluI NaeI NgoMIV NruI PacI PmeI RsrII SanDI SbfI SfiI SgfI SgrAI StuI SwaI Van91I XcmI 载体序列
pCFB, 5292 bp version 013001 NOTE: The following sequence has been verified for accuracy at the junctions. The remainder of the sequence has been obtained from existing data. 1 GAATTGCTAG CAATTGCTAG CAATTGCTAG CAATTCATAC CAGATCACCG 51 AAAACTGTCC TCCAAATGTG TCCCCCTCAC ACTCCCAAAT TCGCGGGCTT 101 CTGCCTCTTA GACCACTCTA CCCTATTCCC CACACTCACC GGAGCCAAAG 151 CCGCGGGACA TATACATGTG AAAGACCCCA CCTGTAGGTT TGGCAAGCTA 201 GCTTAAGTAA CGCCATTTTG CAAGGCATGG AAAAATACAT AACGCATGCC 251 CCATATATGG AGTTCCGCGT TACATAACTT ACGGTAAATG GCCCGCCTGG 301 CTGACCGCCC AACGACCCCC GCCCATTGAC GTCAATAATG ACGTATGTTC 351 CCATAGTAAC GCCAATAGGG ACTTTCCATT GACGTCAATG GGTGGAGTAT 401 TTACGGTAAA CTGCCCACTT GGCAGTACAT CAAGTGTATC ATATGCCAAG 451 TACGCCCCCT ATTGACGTCA ATGACGGTAA ATGGCCCGCC TGGCATTATG 501 CCCAGTACAT GACCTTATGG GACTTTCCTA CTTGGCAGTA CATCTACGTA 551 TTAGTCATCG CTATTACCAT GGTGATGCGG TTTTGGCAGT ACATCAATGG 601 GCGTGGATAG CGGTTTGACT CACGGGGATT TCCAAGTCTC CACCCCATTG 651 ACGTCAATGG GAGTTTGTTT TGGCACCAAA ATCAACGGGA CTTTCCAAAA 701 TGTCGTAACA ACTCCGCCCC ATTGACGCAA ATGGGCGGTA GGCGTGTACG 751 GTGGGAGGTC TATATAAGCA GAGATGCATC CTCACTCGGG GCGCCAGTCC 801 TCCGATTGAC TGAGTCGCCC GGGTACCCGT GTATCCAATA AACCCTCTTG 851 CAGTTGCATC CGACTTGTGG TCTCGCTGTT CCTTGGGAGG GTCTCCTCTG 901 AGTGATTGAC TACCCGTCAG CGGGGGTCTT TCATTTGGGG GCTCGTCCGG 951 GATCGGGAGA CCCCTGCCCA GGGACCACCG ACCCACCACC GGGAGGTAAG 1001 CTGGCCAGCA ACTTATCTGT GTCTGTCCGA TTGTCTAGTG TCTATGACTG 1051 ATTTTATGCG CCTGCGTCGG TACTAGTTAG CTAACTAGCT CTGTATCTGG 1101 CGGACCCGTG GTGGAACTGA CGAGTTCGGA ACACCCGGCC GCAACCCTGG 1151 GAGACGTCCC AGGGACTTCG GGGGCCGTTT TTGTGGCCCG ACCTGAGTCC 1201 AAAAATCCCG ATCGTTTTGG ACTCTTTGGT GCACCCCCCT TAGAGGAGGG 1251 ATATGTGGTT CTGGTAGGAG ACGAGAACCT AAAACAGTTC CCGCCTCCGT 1301 CTGAATTTTT GCTTTCGGTT TGGGACCGAA GCCGCGCCGC GCGTCTTGTC 1351 TGCTGCAGCA TCGTTCTGTG TTGTCTCTGT CTGACTGTGT TTCTGTATTT 1401 GTCTGAAAAT ATGGGCCCGG GCCAGACTGT TACCACTCCC TTAAGTTTGA 1451 CCTTAGGTCA CTGGAAAGAT GTCGAGCGGA TCGCTCACAA CCAGTCGGTA 1501 GATGTCAAGA AGAGACGTTG GGTTACCTTC TGCTCTGCAG AATGGCCAAC 1551 CTTTAACGTC GGATGGCCGC GAGACGGCAC CTTTAACCGA GACCTCATCA 1601 CCCAGGTTAA GATCAAGGTC TTTTCACCTG GCCCGCATGG ACACCCAGAC 1651 CAGGTCCCCT ACATCGTGAC CTGGGAAGCC TTGGCTTTTG ACCCCCCTCC 1701 CTGGGTCAAG CCCTTTGTAC ACCCTAAGCC TCCGCCTCCT CTTCCTCCAT 1751 CCGCCCCGTC TCTCCCCCTT GAACCTCCTC GTTCGACCCC GCCTCGATCC 1801 TCCCTTTATC CAGCCCTCAC TCCTTCTCTA GGCGCCCCCA TATGGCCATA 1851 TGAGATCTTA TATGGGGCAC CCCCGCCCCT TGTAAACTTC CCTGACCCTG 1901 ACATGACAAG AGTTACTAAC AGCCCCTCTC TCCAAGCTCA CTTACAGGCT 1951 CTCTACTTAG TCCAGCACGA AGTCTGGAGA CCTCTGGCGG CACGTACCAA 2001 GAACAACTGG ACCGACCGGT GGTACCTCAC CCTTACCGAG TCGGCGACAC 2051 AGTGTGGGTC CGCCGACACC AGACTAAGAA CCTAGAACCT CGCTGGAAAG 2101 GACCTTACAC AGTCCTGCTG ACCACCCCCA CCGCCCTCAA AGTAGACGGC 2151 ATCGCAGCTT GGATACACGC CGCCCACGTG AAGGCTGCCG ACCCCGGGGG 2201 TGGACCATCC TCTAGACTGC CGGATCGAAT TGTCGACGAA TTCGGATCCT 2251 CGAGCGGCCG CGATCCGAGT TCTTCTGAGC GGGACTCTGG GGTTCGATAA 2301 AATAAAAGAT TTTATTTAGT CTCCAGAAAA AGGGGGGAAT GAAAGACCCC 2351 ACCTGTAGGT TTGGCAAGCT AGCTTAAGTA ACGCCATTTT GCAAGGCATG 2401 GAAAAATACA TAACTGAGAA TAGAGAAGTT CAGATCAAGG TCAGGAACAG 2451 ATGGAACAGC TGAATATGGG CCAAACAGGA TATCTGTGGT AAGCAGTTCC 2501 TGCCCCGGCT CAGGGCCAAG AACAGATGGA ACAGCTGAAT ATGGGCCAAA 2551 CAGGATATCT GTGGTAAGCA GTTCCTGCCC CGGCTCAGGG CCAAGAACAG 2601 ATGGTCCCCA GATGCGGTCC AGCCCTCAGC AGTTTCTAGA GAACCATCAG 2651 ATGTTTCCAG GGTGCCCCAA GGACCTGAAA TGACCCTGTG CCTTATTTGA 2701 ACTAACCAAT CAGTTCGCTT CTCGCTTCTG TTCGCGCGCT TCTGCTCCCC 2751 GAGCTCAATA AAAGAGCCCA CAACCCCTCA CTCGGGGCGC CAGTCCTCCG 2801 ATTGACTGAG TCGCCCGGGT ACCCGTGTAT CCAATAAACC CTCTTGCAGT 2851 TGCATCCGAC TTGTGGTCTC GCTGTTCCTT GGGAGGGTCT CCTCTGAGTG 2901 ATTGACTACC CGTCAGCGGG GGTCTTTCAT TTGGGGGCTC GTCCGGGATC 2951 GGGAGACCCC TGCCCAGGGA CCACCGACCC ACCACCGGGA GGTAAGCTGG 3001 CTGCCTCGCG CGTTTCGGTG ATGACGGTGA AAACCTCTGA CACATGCAGC 3051 TCCCGGAGAC GGTCACAGCT TGTCTGTAAG CGGATGCCGG GAGCAGACAA 3101 GCCCGTCAGG GCGCGTCAGC GGGTGTTGGC GGGTGTCGGG GCGCAGCCAT 3151 GACCCAGTCA CGTAGCGATA GCGGAGTGTA TACTGGCTTA ACTATGCGGC 3201 ATCAGAGCAG ATTGTACTGA GAGTGCACCA TATGCGGTGT GAAATACCGC 3251 ACAGATGCGT AAGGAGAAAA TACCGCATCA GGCGCTCTTC CGCTTCCTCG 3301 CTCACTGACT CGCTGCGCTC GGTCGTTCGG CTGCGGCGAG CGGTATCAGC 3351 TCACTCAAAG GCGGTAATAC GGTTATCCAC AGAATCAGGG GATAACGCAG 3401 GAAAGAACAT GTGAGCAAAA GGCCAGCAAA AGGCCAGGAA CCGTAAAAAG 3451 GCCGCGTTGC TGGCGTTTTT CCATAGGCTC CGCCCCCCTG ACGAGCATCA 3501 CAAAAATCGA CGCTCAAGTC AGAGGTGGCG AAACCCGACA GGACTATAAA 3551 GATACCAGGC GTTTCCCCCT GGAAGCTCCC TCGTGCGCTC TCCTGTTCCG 3601 ACCCTGCCGC TTACCGGATA CCTGTCCGCC TTTCTCCCTT CGGGAAGCGT 3651 GGCGCTTTCT CATAGCTCAC GCTGTAGGTA TCTCAGTTCG GTGTAGGTCG 3701 TTCGCTCCAA GCTGGGCTGT GTGCACGAAC CCCCCGTTCA GCCCGACCGC 3751 TGCGCCTTAT CCGGTAACTA TCGTCTTGAG TCCAACCCGG TAAGACACGA 3801 CTTATCGCCA CTGGCAGCAG CCACTGGTAA CAGGATTAGC AGAGCGAGGT 3851 ATGTAGGCGG TGCTACAGAG TTCTTGAAGT GGTGGCCTAA CTACGGCTAC 3901 ACTAGAAGGA CAGTATTTGG TATCTGCGCT CTGCTGAAGC CAGTTACCTT 3951 CGGAAAAAGA GTTGGTAGCT CTTGATCCGG CAAACAAACC ACCGCTGGTA 4001 GCGGTGGTTT TTTTGTTTGC AAGCAGCAGA TTACGCGCAG AAAAAAAGGA 4051 TCTCAAGAAG ATCCTTTGAT CTTTTCTACG GGGTCTGACG CTCAGTGGAA 4101 CGAAAACTCA CGTTAAGGGA TTTTGGTCAT GAGATTATCA AAAAGGATCT 4151 TCACCTAGAT CCTTTTAAAT TAAAAATGAA GTTTTAAATC AATCTAAAGT 4201 ATATATGAGT AAACTTGGTC TGACAGTTAC CAATGCTTAA TCAGTGAGGC 4251 ACCTATCTCA GCGATCTGTC TATTTCGTTC ATCCATAGTT GCCTGACTCC 4301 CCGTCGTGTA GATAACTACG ATACGGGAGG GCTTACCATC TGGCCCCAGT 4351 GCTGCAATGA TACCGCGAGA CCCACGCTCA CCGGCTCCAG ATTTATCAGC 4401 AATAAACCAG CCAGCCGGAA GGGCCGAGCG CAGAAGTGGT CCTGCAACTT 4451 TATCCGCCTC CATCCAGTCT ATTAATTGTT GCCGGGAAGC TAGAGTAAGT 4501 AGTTCGCCAG TTAATAGTTT GCGCAACGTT GTTGCCATTG CTGCAGGCAT 4551 CGTGGTGTCA CGCTCGTCGT TTGGTATGGC TTCATTCAGC TCCGGTTCCC 4601 AACGATCAAG GCGAGTTACA TGATCCCCCA TGTTGTGCAA AAAAGCGGTT 4651 AGCTCCTTCG GTCCTCCGAT CGTTGTCAGA AGTAAGTTGG CCGCAGTGTT 4701 ATCACTCATG GTTATGGCAG CACTGCATAA TTCTCTTACT GTCATGCCAT 4751 CCGTAAGATG CTTTTCTGTG ACTGGTGAGT ACTCAACCAA GTCATTCTGA 4801 GAATAGTGTA TGCGGCGACC GAGTTGCTCT TGCCCGGCGT CAACACGGGA 4851 TAATACCGCG CCACATAGCA GAACTTTAAA AGTGCTCATC ATTGGAAAAC 4901 GTTCTTCGGG GCGAAAACTC TCAAGGATCT TACCGCTGTT GAGATCCAGT 4951 TCGATGTAAC CCACTCGTGC ACCCAACTGA TCTTCAGCAT CTTTTACTTT 5001 CACCAGCGTT TCTGGGTGAG CAAAAACAGG AAGGCAAAAT GCCGCAAAAA 5051 AGGGAATAAG GGCGACACGG AAATGTTGAA TACTCATACT CTTCCTTTTT 5101 CAATATTATT GAAGCATTTA TCAGGGTTAT TGTCTCATGA GCGGATACAT 5151 ATTTGAATGT ATTTAGAAAA ATAAACAAAT AGGGGTTCCG CGCACATTTC 5201 CCCGAAAAGT GCCACCTGAC GTCTAAGAAA CCATTATTAT CATGACATTA 5251 ACCTATAAAA ATAGGCGTAT CACGAGGCCC TTTCGTCTTC AA
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