MB49-Luc-mCh-Puro Cell Line小鼠膀胱癌细胞萤火虫荧光素酶荧光稳定表达细胞株-BioVector NTCC Inc.

Name：MB49-Luc-mCh-Puro Cell Line小鼠膀胱癌细胞萤火虫荧光素酶荧光稳定表达细胞株-NTCC典型培养物保藏中心

Description

Murine Bladder Carcinoma Cell Line Transfected With Luciferase (MB49-Luc-mCh-Puro) are parental MB49 tumor cells transfected with a Luc expression plasmid using Lipofectamine for luciferase expression. Parental MB49, a murine transitional cell bladder carcinoma line, forms tumors when injected into mouse bladders, either orthotopically or subcutaneously. This cell line is suitable for use in studies concerning bladder carcinoma requiring in vivo tumor imaging.

SpeciesMouse (M. musculus)

Tissue/Organ/Organ SystemRenal

Growth Properties Adherent

Cell Morphology Epithelial

Seeding Density40,000 - 120,000 cells/cm²; split ratio of 1:5 or 1:10

Population Doubling Time22 - 26 hours

Applications

For Research Use Only

Unit quantity1x10⁶cells / 1.0 ml

Cell TypeDrug Discovery Cell Lines

Expression Profile

Luciferase

Propagation Requirements

Use of PriCoatT25 Flasksor Applied Cell Extracellular Matrixs required for cell adhesion to the culture vessels. Grow cells in ECM-coated culture vessels unless otherwise specified in the Propagation Requirements below.

The base medium for this cell line is Prigrow III medium available at BioVector, Cat. No. BioVector-TM003. To make the complete growth medium, add the following components to the base medium: heat-inactivated fetal bovine serum (BioVector-TM999) to a final concentration of 10%, Penicillin/Streptomycin Solution (BioVector-G255) to a final concentration of 1%, NEAA to a final concentration of 1X, sodium pyruvate (BioVector-TM057) to a final concentration of 1 mM, and L-glutamine (BioVector-G275) to a final concentration of 2 mM.

Carbon dioxide (CO2): 5%, Temperature: 37.0°C.

【Organism物种来源】Animal  
【Tissue组织来源】
【Cell Type细胞类型】  
【Product Format产品状态】 frozen/live culture
【Morphology细胞形态】  
【Culture Properties细胞特性】
【Biosafety Level生物安全级别】 1/2
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.

【Disease细胞源疾病】
【Age年龄】
【Gender性别】 Male/Female
【Storage Conditions保存条件】 liquid nitrogen vapor phase
【Karyotype染色体组型】
【Images细胞图片】 Cell Micrograph
【Derivation衍生细胞】 ­
【Clinical Data临床数据】
【Comments其他描述】
【Complete Growth Medium完全培养基】 The base medium for this cell line is DMEM/RPMI-1640 Medium. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.
【Subculturing传代方法】
Volumes are given for a 75 cm2 flask. Increase or decrease the amount of dissociation medium needed proportionally for culture vessels of other sizes.
Remove and discard culture medium.
Briefly rinse the cell layer with 0.25% (w/v) Trypsin- 0.53 mM EDTA solution to remove all traces of serum that contains trypsin inhibitor.
Add 2.0 to 3.0 mL of Trypsin-EDTA solution to flask and observe cells under an inverted microscope until cell layer is dispersed (usually within 5 to 15 minutes).
Note: To avoid clumping do not agitate the cells by hitting or shaking the flask while waiting for the cells to detach. Cells that are difficult to detach may be placed at 37°C to facilitate dispersal.
Add 6.0 to 8.0 mL of complete growth medium and aspirate cells by gently pipetting.
Add appropriate aliquots of the cell suspension to new culture vessels.
Incubate cultures at 37°C.
Subcultivation Ratio: A subcultivation ratio of 1:2 to 1:6 is recommended
Medium Renewal: 2 to 3 times per week
【Cryopreservation冻存条件】
【Freeze Medium冻存培养基】Complete growth medium supplemented with 5% (v/v) DMSO
【Storage Temperature保存温度】 Liquid nitrogen vapor phase
【Culture Conditions培养条件】
【Atmosphere培养环境】 Air, 95%; carbon dioxide (CO2), 5%
【Temperature培养温度】 37°C
【STR Profile图谱】
【Population Doubling Time倍增殖时间】
【References参考文献】

【Supplier细胞供应厂家】BioVector NTCC Inc.
【Website网址】http://www.biovector.net

Supplier供应商:BioVector质粒载体菌株细胞蛋白抗体基因保藏中心
NTCC典型培养物保藏中心
http://www.biovector.net