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Biomass生物质能源利用相关基因质粒,纤维素糖化产能-BioVector NTCC保藏中心

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  • 货  号:BVBiomass
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Biomass Collection

It is one of the key issues to utilize the biomass for the worldwide energy crisis. The RIKEN BioResource Center (BRC) has collected numbers of genes that are related to cellulose saccharification and has constructed expression plasmids of those genes to express recombinant proteins in E. coli. Currently, 25 genes were collected from filamentous fungus Trichoderma reesei (Hypocrea jecorina: JCM 22676) and Pyrococcus furiosus (JCM 8422T). And then, 38 expression plasmids are also distributed. For example, recombinant endo-type cellulase EGII-His or EG-III-His was obtained from E. coli with the expression clone pET21c_Tr-egl2-His (RDB08289) or pET21c_Tr-egl3-His (RDB08290) which was subcloned the gene (egl2 or egl3) from T. reesei. EG2-His or EG3-His in crude extract indicated the activity at 12.2 U/ml and 4.6 U/ml, respectively (One ml of crude extract was prepared from 10 ml cultured E. coli). The information associated with plasmids, such as a nucleotide sequence and digestion pattern by restriction endonucleases. Furthermore, protocols of expression of recombinant proteins in E. coli are available on this website.

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in Expression vector

Name of cloneCat no.
pET25b_Tr-agl2-His
 Expression vector of alpha -Galactosidase 2 Agl2 of Trichoderma reesei
RDB08535
pET25b_Tr-agl3-His
 Expression vector of alpha -Galactosidase 3 Agl3 of Trichoderma reesei
RDB08536
pET25b_Tr-abf1-His
 Expression vector of arabinofuranosidase 1 Abf1 of Trichoderma reesei
RDB08534
pET21c_Tr-bgl1-His
 Expression vector of beta-D-glucoside glucohydrolase 1 of Trichoderma reesei
RDB08291
pET25b_Tr-bgl1-His
 Expression vector of beta-D-glucoside glucohydrolase 1 of Trichoderma reesei
RDB08311
pET25b_Tr-bxl1-His
 Expression vector of beta-xylosidase Bxl1 of Trichoderma reesei
RDB08533
pET25b_Tr-cip1-His
 Expression vector of Cip1 of Trichoderma reesei
RDB08537
pET25b_Tr-cip2-His
 Expression vector of Cip2 of Trichoderma reesei
RDB08538
pET21c_Tr-xyn1-His
 Expression vector of Endo-1,4-beta-xylanase 1 (Xylanase 1) of Trichoderma reesei
RDB08292
pET25b_Tr-xyn1-His
 Expression vector of Endo-1,4-beta-xylanase 1 (Xylanase 1) of Trichoderma reesei
RDB08312
pET21c_Tr-xyn2-His
 Expression vector of Endo-1,4-beta-xylanase 2 (Xylanase 2) of Trichoderma reesei
RDB08293
pET25b_Tr-xyn2-His
 Expression vector of Endo-1,4-beta-xylanase 2 (Xylanase 2) of Trichoderma reesei
RDB08313
pET21c_Tr-cel5b-His
 Expression vector of Endoglucanase Cel5b of Trichoderma reesei
RDB08296
pET25b_Tr-cel5b-His
 Expression vector of Endoglucanase Cel5b of Trichoderma reesei
RDB08316
pET21c_Tr-cel61b-His
 Expression vector of Endoglucanase Cel61b of Trichoderma reesei
RDB08297
pET25b_Tr-cel61b-His
 Expression vector of Endoglucanase Cel61b of Trichoderma reesei
RDB08317
pET21c_Tr-cel74a-His
 Expression vector of Endoglucanase Cel74a of Trichoderma reesei
RDB08298
pET25b_Tr-cel74a-His
 Expression vector of Endoglucanase Cel74a of Trichoderma reesei
RDB08318
pET21c_Tr-egl5-His
 Expression vector of Endoglucanase EG-5 of Trichoderma reesei
RDB08299
pET25b_Tr-egl5-His
 Expression vector of Endoglucanase EG-5 of Trichoderma reesei
RDB08319
pET21c_Tr-egl1-His
 Expression vector of Endoglucanase EG-I of Trichoderma reesei
RDB08288
pET25b_Tr-egl1-His
 Expression vector of Endoglucanase EG-I of Trichoderma reesei
RDB08308
pET21c_Tr-egl2-His
 Expression vector of Endoglucanase EG-II of Trichoderma reesei
RDB08289
pET25b_Tr-egl2-His
 Expression vector of Endoglucanase EG-II of Trichoderma reesei
RDB08309
pET21c_Tr-egl3-His
 Expression vector of Endoglucanase EG-III of Trichoderma reesei
RDB08290
pET25b_Tr-egl3-His
 Expression vector of Endoglucanase EG-III of Trichoderma reesei
RDB08310
pET21c_Tr-cbh1-His
 Expression vector of Exoglucanase 1 of Trichoderma reesei
RDB08286
pET25b_Tr-cbh1-His
 Expression vector of Exoglucanase 1 of Trichoderma reesei
RDB08306
pET21c_Tr-cbh2-His
 Expression vector of Exoglucanase 2 of Trichoderma reesei
RDB08287
pET25b_Tr-cbh2-His
 Expression vector of Exoglucanase 2 of Trichoderma reesei
RDB08307
pET21c_Tr-swo1-His
 Expression vector of swollenin of Trichoderma reesei
RDB08295
pET25b_Tr-swo1-His
 Expression vector of swollenin of Trichoderma reesei
RDB08315
pET21c_Tr-xyn3-His
 Expression vector of xylanase III of Trichoderma reesei
RDB08294
pET25b_Tr-xyn3-His
 Expression vector of xylanase III of Trichoderma reesei
RDB08314
pET21c_HAT-PF0369
 Expression clone of Pyrococcus furiosus endoglucanase
RDB08963
pET25b_PF0369-His
 Expression clone of Pyrococcus furiosus endoglucanase
RDB08954
pET21c_HAT-PF0854
 Expression clone of Pyrococcus furiosus endoglucanase
RDB08959
pET25b_PF0854-His
 Expression clone of Pyrococcus furiosus endoglucanase
RDB08950
pET21c_HAT-PF1547
 Expression clone of Pyrococcus furiosus endoglucanase
RDB08960
pET25b_PF1547-His
 Expression clone of Pyrococcus furiosus endoglucanase
RDB08951
pET21c_HAT-PF1861
 Expression clone of Pyrococcus furiosus endoglucanase
RDB08961
pET25b_PF1861-His
 Expression clone of Pyrococcus furiosus endoglucanase
RDB08952

clone_table2.pl-biomass130801.csv 2013.08.01

in Cloning vector

Name of cloneCat no.
pBSII_Tr-agl2
 Plasmid clone of Trichoderma reesei alpha-galactosidase 2
RDB08142
pBSII_Tr-agl3
 Plasmid clone of Trichoderma reesei alpha-galactosidase 3
RDB08143
pBSII_Tr-abf1
 Plasmid clone of Trichoderma reesei arabinosidase
RDB08141
pBSII_Tr-bgl1
 Plasmid clone of Trichoderma reesei beta-D-glucoside glucohydrolase 1
RDB08136
pBSII_Tr-bxl1
 Plasmid clone of Trichoderma reesei beta-xylosidase 1
RDB08140
pBSII_Tr-cip1
 Plasmid clone of Trichoderma reesei cip1
RDB08145
pBSII_Tr-cip2
 Plasmid clone of Trichoderma reesei cip2
RDB08146
pBSII_Tr-cel5b
 Plasmid clone of Trichoderma reesei endoglucanase Cel5b
RDB08147
pBSII_Tr-cel61b
 Plasmid clone of Trichoderma reesei endoglucanase Cel61b
RDB08148
pBSII_Tr-cel74a
 Plasmid clone of Trichoderma reesei endoglucanase Cel74a
RDB08149
pBSII_Tr-egl5
 Plasmid clone of Trichoderma reesei endoglucanase EG-5
RDB08150
pBSII_Tr-egl1
 Plasmid clone of Trichoderma reesei endoglucanase EG-I
RDB08133
pBSII_Tr-egl2
 Plasmid clone of Trichoderma reesei endoglucanase EG-II
RDB08134
pBSII_Tr-egl3
 Plasmid clone of Trichoderma reesei endoglucanase EG-III
RDB08135
pBSII_Tr-cbh1
 Plasmid clone of Trichoderma reesei exoglucanase 1
RDB08131
pBSII_Tr-cbh2
 Plasmid clone of Trichoderma reesei exoglucanase 2
RDB08132
pBSII_Tr-swo1
 Plasmid clone of Trichoderma reesei swollenin
RDB08144
pBSII_Tr-xyn1
 Plasmid clone of Trichoderma reesei xylanase 1
RDB08137
pBSII_Tr-xyn2
 Plasmid clone of Trichoderma reesei xylanase 2
RDB08138
pBSII_Tr-xyn3
 Plasmid clone of Trichoderma reesei xylanase III
RDB08139
pBSII_PF0369
 Plasmid clone of Pyrococcus furiosus endoglucanase
RDB08949
pBSII_PF0854
 Plasmid clone of Pyrococcus furiosus endoglucanase
RDB08945
pBSII_PF1547
 Plasmid clone of Pyrococcus furiosus endoglucanase
RDB08946
pBSII_PF1861
 Plasmid clone of Pyrococcus furiosus endoglucanase
RDB08947


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