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Cell line:
JURKAT
Cat# NTCC-SCSP513
Species:
human (Homo sapiens)
Cell type:
T cell leukemia
Origin:
established from the peripheral blood of a 14-year-old boy with acute lymphoblastic leukemia (ALL) at first relapse in 1976; often this cell line is
called "JM" (JURKAT and JM are derived from the same patient and are sister clones), occasionally JM may be a subclone with somewhat
divergent features
Biosafety level:
1
Permissions and restrictions:
A
NTCC Cell Culture Data:
Morphology:
round cells growing singly or in clumps in suspension; image;image
Medium:
90% RPMI 1640 + 10% h.i. FBS + 2 mM L-glutamine
Subculture:
optimal split ratio of about 1:2 to 1:3 every 2-3 days; seed out after thawing at about 1 x 106cells/ml; maintain at 0.5-1.5 x 106cells/ml
Incubation:
at 37 °C with 5% CO2
Doubling time:
about 25-35 hours
Harvest:
maximal density at about 1.5 x 106cells/ml
Storage:
frozen with 70% medium, 20% FBS, 10% DMSO
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NTCC Scientific Data:
Mycoplasma:
contamination was eliminated with Mycoplasma Removal Agent, then negative in DAPI, microbiological culture, RNA hybridization, PCR assays
Immunology:
CD2 +, CD3 +, CD5 +, CD6 +, CD7 +, CD8 -, CD13 -, CD19 -, TCRalpha/beta +, TCRgamma/delta -; image
Fingerprint:
multiplex PCR of minisatellite markers revealed a unique DNA profile
Species:
confirmed as human with IEF of AST, LDH, NP
Cytogenetics:
human flat-moded hypotetraploid karyotype with 7.8% polyploidy - 87(78-91)<4n>XX, -Y, -Y, -5, -16, -17, -22, add(2)(p21)/del(2)(p23)x2 - sideline
with additional der(5)t(5;10)(q11;p15), del(9)(p11)
Viruses:
ELISA: reverse transcriptase negative; PCR: EBV -, HBV -, HCV -, HHV-8 -, HIV-1 -, HIV-2 -, HTLV-I/II -, MLV -, SMRV -
Jurkat 【中文名称】： 人白血病T淋巴细胞
【保藏编号】： NTCC-SCSP513
【来源】： 人
【供应机构】： BioVector NTCC典型培养物保藏中心
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