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裂殖酵母载体列表S. pombe Vector Series-BioVector NTCC保藏中心

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List of S. pombe Vector Series


See also NCBI VECTORS

S. pombe expression vectors (Gateway compatible)

Reference

  • Matsuyama A, Shirai A, Yashiroda Y, Kamata A, Horinouchi S, Yoshida M. pDUAL, a multipurpose, multicopy vector capable of chromosomal integration in fission yeast. Yeast. 2004 Nov;21(15):1289-305.

  • Matsuyama A, Arai R, Yashiroda Y, Shirai A, Kamata A, Sekido S, Kobayashi Y, Hashimoto A, Hamamoto M, Hiraoka Y, Horinouchi S, Yoshida M. ORFeome cloning and global analysis of protein localization in the fission yeast Schizosaccharomyces pombe. Nature Biotechnol. 2006 Jul; 24(7):841-7.

  • Matsuyama A, Shirai A, Yoshida M. A series of promoters for constitutive expression of heterologous genes in fission yeast. Yeast 2008 May;25(5):371-6.

  • Matsuyama A, Shirai A, Yoshida M. A novel series of vectors for chromosomal integration in fission yeast. Biochem Biophys Res Commun. 2008 Sep 19;374(2): 315-9.


Expression Vector Backbone of Schizosaccharomyces pombe

Depositor: Dr. Toshiaki Isobe
Reference: PLoS One 9, e112488 (2014)., PMID 25401760.

Catalog No.plasmidbackborneSp markerEc markerinsertremarks
RDB08041pCtrlki-kanMX6bacterialkanMX6ApMCS1-opal stop codon-kanMX6-MCS2Plasmid vector to constract C-terminal truncated allele by the integration of opal stop codon into target gene using homologous recombination.
RDB08042pCtFEM3ki-kanMX6bacterialkanMX6ApMCS1-Flag-tev-3myc-opal stop codon-kanMX6-MCS2Plasmid vector to construct C-terminally FEM3 (FLAG-tev-3xmyc) tagged allele of target gene by using homologous recombination.
RDB08045pCtFLATAki-kanMX6bacterialkanMX6ApMCS1-Flag-tev-protein A-opal stop codon-kanMX6-MCS2Plasmid vector to construct C-terminally FLATA (FLAG-tev-protein A) tagged allele of target gene by using homologous recombination.
RDB08048pFOX1-CFLATAreplicativeScLEU2(complement leu1-)ApPtef-MCS1-Flag-tev-protein A-opal stop codon-TtefPlasmid vector to constract replicative expression clone constitutively expressing C-terminally FLATA tag (FLAG-tev-protein A) allele in leu1- strain of S. pombe.
RDB08051pCtHATAki-kanMX6bacterialkanMX6ApMCS1-HA-tev-protein A-opal stop codon-kanMX6-MCS2Plasmid vector to constract C-terminally HATA (HA-tev-protein A) tagged allele of target gene by using homologous recombination.
RDB08052pFOX1-CHATAreplicativeScLEU2(complement leu1-)ApPtef-MCS1-HA-tev-protein A-opal stop codon-TtefPlasmid vector to constract replicative expression clone constitutively expressing C-terminally HATA tag (HA-tev-protein A) allele in leu1- strain of S. pombe.

pDUAL series

Vector sequence is abailable at Chemical Genetics Laboratory.

pDUAL series (Gateway-compatible)

Cat. num.
Plasmid name
Backbone
Tag
Tag
position
Promoter
Multicopy marker
Integration marker

RDB06130pDUAL-HFF1c
pDUAL
His6-FLAG2
5'
nmt1
ura4+
leu1+
[sequence]
RDB06485pDUAL-HFF21c
pDUAL
His6-FLAG2
5'
tub1
ura4+
leu1+
[sequence]
RDB06486pDUAL-HFF31c
pDUAL
His6-FLAG2
5'
cam1
ura4+
leu1+
[sequence]
RDB06131pDUAL-HFF41c
pDUAL
His6-FLAG2
5'
nmt1*
ura4+
leu1+
[sequence]
RDB06487pDUAL-HFF51c
pDUAL
His6-FLAG2
5'
tif51
ura4+
leu1+
[sequence]
RDB06488pDUAL-HFF61c
pDUAL
His6-FLAG2
5'
ef1a-c
ura4+
leu1+
[sequence]
RDB06132pDUAL-HFF81c
pDUAL
His6-FLAG2
5'
nmt1**
ura4+
leu1+
[sequence]
RDB06133pDUAL-HFG1c
pDUAL
His6-FLAG-GFP(S65C)
5'
nmt1
ura4+
leu1+
[sequence]
RDB06493pDUAL-HFG21c
pDUAL
His6-FLAG-GFP(S65C)
5'
tub1
ura4+
leu1+
[sequence]
RDB06494pDUAL-HFG31c
pDUAL
His6-FLAG-GFP(S65C)
5'
cam1
ura4+
leu1+
[sequence]
RDB06134pDUAL-HFG41c
pDUAL
His6-FLAG-GFP(S65C)
5'
nmt1*
ura4+
leu1+
[sequence]
RDB06495pDUAL-HFG51c
pDUAL
His6-FLAG-GFP(S65C)
5'
tif51
ura4+
leu1+
[sequence]
RDB06496pDUAL-HFG61c
pDUAL
His6-FLAG-GFP(S65C)
5'
ef1a-c
ura4+
leu1+
[sequence]
RDB06135pDUAL-HFG81c
pDUAL
His6-FLAG-GFP(S65C)
5'
nmt1**
ura4+
leu1+
[sequence]
RDB06136pDUAL-HFC1c
pDUAL
His6-FLAG-CFP
5'
nmt1
ura4+
leu1+
[sequence]
RDB06137pDUAL-HFC41c
pDUAL
His6-FLAG-CFP
5'
nmt1*
ura4+
leu1+
[sequence]
RDB06138pDUAL-HFC81c
pDUAL
His6-FLAG-CFP
5'
nmt1**
ura4+
leu1+
[sequence]
RDB06139pDUAL-HFY1c
pDUAL
His6-FLAG-YFP
5'
nmt1
ura4+
leu1+
[sequence]
RDB06140pDUAL-HFY41c
pDUAL
His6-FLAG-YFP
5'
nmt1*
ura4+
leu1+
[sequence]
RDB06141pDUAL-HFY81c
pDUAL
His6-FLAG-YFP
5'
nmt1**
ura4+
leu1+
[sequence]
RDB06142pDUAL-FFH1c
pDUAL
FLAG2-His6
3'
nmt1
ura4+
leu1+
[sequence]
RDB06489pDUAL-FFH21c
pDUAL
FLAG2-His6
3'
tub1
ura4+
leu1+
[sequence]
RDB06490pDUAL-FFH31c
pDUAL
FLAG2-His6
3'
cam1
ura4+
leu1+
[sequence]
RDB06143pDUAL-FFH41c
pDUAL
FLAG2-His6
3'
nmt1*
ura4+
leu1+
[sequence]
RDB06491pDUAL-FFH51c
pDUAL
FLAG2-His6
3'
tif51
ura4+
leu1+
[sequence]
RDB06492pDUAL-FFH61c
pDUAL
FLAG2-His6
3'
ef1a-c
ura4+
leu1+
[sequence]
RDB06144pDUAL-FFH81c
pDUAL
FLAG2-His6
3'
nmt1**
ura4+
leu1+
[sequence]
RDB06145pDUAL-GFH1c
pDUAL
GFP(S65C)-FLAG-His6
3'
nmt1
ura4+
leu1+
[sequence]
RDB06497pDUAL-GFH21c
pDUAL
GFP(S65C)-FLAG-His6
3'
tub1
ura4+
leu1+
[sequence]
RDB06498pDUAL-GFH31c
pDUAL
GFP(S65C)-FLAG-His6
3'
cam1
ura4+
leu1+
[sequence]
RDB06146pDUAL-GFH41c
pDUAL
GFP(S65C)-FLAG-His6
3'
nmt1*
ura4+
leu1+
[sequence]
RDB06499pDUAL-GFH51c
pDUAL
GFP(S65C)-FLAG-His6
3'
tif51
ura4+
leu1+
[sequence]
RDB06500pDUAL-GFH61c
pDUAL
GFP(S65C)-FLAG-His6
3'
ef1a-c
ura4+
leu1+
[sequence]
RDB06147pDUAL-GFH81c
pDUAL
GFP(S65C)-FLAG-His6
3'
nmt1**
ura4+
leu1+
[sequence]
RDB06148pDUAL-CFH1c
pDUAL
CFP-FLAG-His6
3'
nmt1
ura4+
leu1+
[sequence]
RDB06149pDUAL-CFH41c
pDUAL
CFP-FLAG-His6
3'
nmt1*
ura4+
leu1+
[sequence]
RDB06150pDUAL-CFH81c
pDUAL
CFP-FLAG-His6
3'
nmt1**
ura4+
leu1+
[sequence]
RDB06151pDUAL-YFH1c
pDUAL
YFP-FLAG-His6
3'
nmt1
ura4+
leu1+
[sequence]
RDB06152pDUAL-YFH41c
pDUAL
YFP-FLAG-His6
3'
nmt1*
ura4+
leu1+
[sequence]
RDB06153pDUAL-YFH81c
pDUAL
YFP-FLAG-His6
3'
nmt1**
ura4+
leu1+
[sequence]
RDB06481pDUAL-FFH1c rfA
pDUAL
FLAG2-His6
3'
nmt1
ura4+
leu1+
[sequence]
RDB06482pDUAL-FFH1c rfC
pDUAL
FLAG2-His6
3'
nmt1
ura4+
leu1+
[sequence]
RDB06483pDUAL-YFH1c rfA
pDUAL
YFP-FLAG-His6
3'
nmt1
ura4+
leu1+
[sequence]
RDB06484pDUAL-YFH1c rfC
pDUAL
YFP-FLAG-His6
3'
nmt1
ura4+
leu1+
[sequence]

* Medium strength nmt1 promoter, ** Low strength nmt1 promoter

pDUAL2 series

Vector sequence is abailable at Chemical Genetics Laboratory.

pDUAL2 series (Gateway-compatible)

Cat. num.
Plasmid name
Backbone
Tag
Tag
position
Promoter
Multicopy marker
Integration marker

RDB06154pDUAL2-HFF1c
pDUAL2
His6-FLAG2
5'
nmt1
ura4+
ura4+, leu1-
[sequence]
RDB06155pDUAL2-HFF41c
pDUAL2
His6-FLAG2
5'
nmt1*
ura4+
ura4+, leu1-
[sequence]
RDB06156pDUAL2-HFF81c
pDUAL2
His6-FLAG2
5'
nmt1**
ura4+
ura4+, leu1-
[sequence]
RDB06157pDUAL2-HFG1c
pDUAL2
His6-FLAG-GFP(S65C)
5'
nmt1
ura4+
ura4+, leu1-
[sequence]
RDB06158pDUAL2-HFG41c
pDUAL2
His6-FLAG-GFP(S65C)
5'
nmt1*
ura4+
ura4+, leu1-
[sequence]
RDB06159pDUAL2-HFG81c
pDUAL2
His6-FLAG-GFP(S65C)
5'
nmt1**
ura4+
ura4+, leu1-
[sequence]
RDB06160pDUAL2-HFC1c
pDUAL2
His6-FLAG-CFP
5'
nmt1
ura4+
ura4+, leu1-
[sequence]
RDB06161pDUAL2-HFC41c
pDUAL2
His6-FLAG-CFP
5'
nmt1*
ura4+
ura4+, leu1-
[sequence]
RDB06162pDUAL2-HFC81c
pDUAL2
His6-FLAG-CFP
5'
nmt1**
ura4+
ura4+, leu1-
[sequence]
RDB06163pDUAL2-HFY1c
pDUAL2
His6-FLAG-YFP
5'
nmt1
ura4+
ura4+, leu1-
[sequence]
RDB06164pDUAL2-HFY41c
pDUAL2
His6-FLAG-YFP
5'
nmt1*
ura4+
ura4+, leu1-
[sequence]
RDB06165pDUAL2-HFY81c
pDUAL2
His6-FLAG-YFP
5'
nmt1**
ura4+
ura4+, leu1-
[sequence]
RDB06166pDUAL2-FFH1c
pDUAL2
FLAG2-His6
3'
nmt1
ura4+
ura4+, leu1-
[sequence]
RDB06167pDUAL2-FFH41c
pDUAL2
FLAG2-His6
3'
nmt1*
ura4+
ura4+, leu1-
[sequence]
RDB06168pDUAL2-FFH81c
pDUAL2
FLAG2-His6
3'
nmt1**
ura4+
ura4+, leu1-
[sequence]
RDB06169pDUAL2-GFH1c
pDUAL2
GFP(S65C)-FLAG-His6
3'
nmt1
ura4+
ura4+, leu1-
[sequence]
RDB06170pDUAL2-GFH41c
pDUAL2
GFP(S65C)-FLAG-His6
3'
nmt1*
ura4+
ura4+, leu1-
[sequence]
RDB06171pDUAL2-GFH81c
pDUAL2
GFP(S65C)-FLAG-His6
3'
nmt1**
ura4+
ura4+, leu1-
[sequence]
RDB06172pDUAL2-CFH1c
pDUAL2
CFP-FLAG-His6
3'
nmt1
ura4+
ura4+, leu1-
[sequence]
RDB06173pDUAL2-CFH41c
pDUAL2
CFP-FLAG-His6
3'
nmt1*
ura4+
ura4+, leu1-
[sequence]
RDB06174pDUAL2-CFH81c
pDUAL2
CFP-FLAG-His6
3'
nmt1**
ura4+
ura4+, leu1-
[sequence]
RDB06175pDUAL2-YFH1c
pDUAL2
YFP-FLAG-His6
3'
nmt1
ura4+
ura4+, leu1-
[sequence]
RDB06503pDUAL2-YFH21c
pDUAL2
YFP-FLAG-His6
3'
nmt1
ura4+
ura4+, leu1-
[sequence]
RDB06176pDUAL2-YFH41c
pDUAL2
YFP-FLAG-His6
3'
nmt1*
ura4+
ura4+, leu1-
[sequence]
RDB06177pDUAL2-YFH81c
pDUAL2
YFP-FLAG-His6
3'
nmt1**
ura4+
ura4+, leu1-
[sequence]
RDB06501pDUAL2-BFH41c
pDUAL2
BFP-FLAG-His6
3'
nmt1*
ura4+
ura4+, leu1-
[sequence]
RDB06502pDUAL2-BFH81c
pDUAL2
BFP-FLAG-His6
3'
nmt1**
ura4+
ura4+, leu1-
[sequence]

* Medium strength nmt1 promoter, ** Low strength nmt1 promoter

pREP series

Vector sequence is abailable at Chemical Genetics Laboratory.

pREP series (Gateway-compatible)

Cat. num.
Plasmid name
Backbone
Tag
Tag
position
Promoter
Selection marker

RDB06519pREP1-ccdB2
pREP1
-
-
nmt1
LEU2
[sequence]
RDB06520pREP41-ccdB2
pREP41
-
-
nmt1*
LEU2
[sequence]
RDB06521pREP81-ccdB2
pREP81
-
-
nmt1**
LEU2
[sequence]
RDB06522pHFF1-ccdB2
pREP1
His6-FLAG2
5'
nmt1
LEU2
[sequence]
RDB06523pHFF41-ccdB2
pREP41
His6-FLAG2
5'
nmt1*
LEU2
[sequence]
RDB06524pHFF81-ccdB2
pREP81
His6-FLAG2
5'
nmt1**
LEU2
[sequence]
RDB06525pHFG1-ccdB2
pREP1
His6-FLAG-GFP(S65C)
5'
nmt1
LEU2
[sequence]
RDB06526pHFG41-ccdB2
pREP41
His6-FLAG-GFP(S65C)
5'
nmt1*
LEU2
[sequence]
RDB06527pHFG81-ccdB2
pREP81
His6-FLAG-GFP(S65C)
5'
nmt1**
LEU2
[sequence]
RDB06506pFFH1-ccdB2
pREP1
FLAG2-His6
3'
nmt1
LEU2
[sequence]
RDB06507pFFH41-ccdB2
pREP41
FLAG2-His6
3'
nmt1*
LEU2
[sequence]
RDB06508pFFH81-ccdB2
pREP81
FLAG2-His6
3'
nmt1**
LEU2
[sequence]
RDB06509pGFH1-ccdB2
pREP1
GFP(S65C)-FLAG-His6
3'
nmt1
LEU2
[sequence]
RDB06510pGFH41-ccdB2
pREP41
GFP(S65C)-FLAG-His6
3'
nmt1*
LEU2
[sequence]
RDB06511pGFH81-ccdB2
pREP81
GFP(S65C)-FLAG-His6
3'
nmt1**
LEU2
[sequence]
RDB06513pHFY1-ccdB2
pREP1
His6-FLAG-YFP
5'
nmt1
LEU2
[sequence]
RDB06512pHFC1-ccdB2
pREP1
His6-FLAG-CFP
5'
nmt1
LEU2
[sequence]
RDB06515pYFH1-ccdB2
pREP1
YFP-FLAG-His6
3'
nmt1
LEU2
[sequence]
RDB06505pYFH1-ccdB2 rfA
pREP1
YFP-FLAG-His6
3'
nmt1
LEU2
[sequence]
RDB06504pYFH1-ccdB2 rfC
pREP1
YFP-FLAG-His6
3'
nmt1
LEU2
[sequence]
RDB06514pCFH1-ccdB2
pREP1
CFP-FLAG-His6
3'
nmt1
LEU2
[sequence]

* Medium strength nmt1 promoter, ** Low strength nmt1 promoter

Integration vectors

Vector sequence is abailable at Chemical Genetics Laboratory.

Gateway-compatible vectors for chromosomal integration

Cat. num.
Plasmid name
Backbone
Promoter
Tag
Tag
position
Multicopy marker
Targeted locus
(negative) selection
Positive selection marker

RDB06462pLYS1U-CFH1c
pUC119
nmt1
CFP-FLAG-His6
3'
-
lys1+
ura4+
[sequence]
RDB06463pLYS1U-CFH21c
pUC119
tub1
CFP-FLAG-His6
3'
-
lys1+
ura4+
[sequence]
RDB06464pLYS1U-CFH61c
pUC119
ef1a-c
CFP-FLAG-His6
3'
-
lys1+
ura4+
[sequence]
RDB06465pLYS1U-FFH1c
pUC119
nmt1
FLAG2-His6
3'
-
lys1+
ura4+
[sequence]
RDB06466pLYS1U-GFH1c
pUC119
nmt1
GFP(S65C)-FLAG-His6
3'
-
lys1+
ura4+
[sequence]
RDB06467pLYS1U-GFH21c
pUC119
tub1
GFP(S65C)-FLAG-His6
3'
-
lys1+
ura4+
[sequence]
RDB06468pLYS1U-GFH61c
pUC119
ef1a-c
GFP(S65C)-FLAG-His6
3'
-
lys1+
ura4+
[sequence]
RDB06477pLYS1U-YFH1c
pUC119
nmt1
YFP-FLAG-His6
3'
-
lys1+
ura4+
[sequence]
RDB06478pLYS1U-YFH21c
pUC119
tub1
YFP-FLAG-His6
3'
-
lys1+
ura4+
[sequence]
RDB06479pLYS1U-YFH61c
pUC119
ef1a-c
YFP-FLAG-His6
3'
-
lys1+
ura4+
[sequence]
RDB06469pLYS1U-HFC1c
pUC119
nmt1
His6-FLAG-CFP
5'
-
lys1+
ura4+
[sequence]
RDB06470pLYS1U-HFC21c
pUC119
tub1
His6-FLAG-CFP
5'
-
lys1+
ura4+
[sequence]
RDB06471pLYS1U-HFC61c
pUC119
ef1a-c
His6-FLAG-CFP
5'
-
lys1+
ura4+
[sequence]
RDB06472pLYS1U-HFF1c
pUC119
nmt1
His6-FLAG2
5'
-
lys1+
ura4+
[sequence]
RDB06473pLYS1U-HFG1c
pUC119
nmt1
His6-FLAG-GFP(S65C)
5'
-
lys1+
ura4+
[sequence]
RDB06474pLYS1U-HFY1c
pUC119
nmt1
His6-FLAG-YFP
5'
-
lys1+
ura4+
[sequence]
RDB06475pLYS1U-HFY21c
pUC119
tub1
His6-FLAG-YFP
5'
-
lys1+
ura4+
[sequence]
RDB06476pLYS1U-HFY61c
pUC119
ef1a-c
His6-FLAG-YFP
5'
-
lys1+
ura4+
[sequence]
RDB06458pLYS1K-FFH1c
pUC119
nmt1
FLAG2-His6
3'
-
lys1+
kanr
[sequence]
RDB06459pLYS1K-GFH1c
pUC119
nmt1
GFP(S65C)-FLAG-His6
3'
-
lys1+
kanr
[sequence]
RDB06460pLYS1K-HFF1c
pUC119
nmt1
His6-FLAG2
5'
-
lys1+
kanr
[sequence]
RDB06461pLYS1K-HFG1c
pUC119
nmt1
His6-FLAG-GFP(S65C)
5'
-
lys1+
kanr
[sequence]
RDB06517pHIS3U-CFH1c
pUC119
nmt1
CFP-FLAG-His6
3'
-
his3+
ura4+
[sequence]
RDB06455pHIS3U-GFH1c
pUC119
nmt1
GFP(S65C)-FLAG-His6
3'
-
his3+
ura4+
[sequence]
RDB06518pHIS3U-YFH1c
pUC119
nmt1
YFP-FLAG-His6
3'
-
his3+
ura4+
[sequence]
RDB06456pHIS3U-HFF1c
pUC119
nmt1
His6-FLAG2
5'
-
his3+
ura4+
[sequence]
RDB06516pHIS3U-HFC1c
pUC119
nmt1
His6-FLAG-CFP
5'
-
his3+
ura4+
[sequence]
RDB06457pHIS3U-HFG1c
pUC119
nmt1
His6-FLAG-GFP(S65C)
5'
-
his3+
ura4+
[sequence]
RDB06445pHIS3K-CFH1c
pUC119
nmt1
CFP-FLAG-His6
3'
-
his3+
kanr
[sequence]
RDB06446pHIS3K-CFH21c
pUC119
tub1
CFP-FLAG-His6
3'
-
his3+
kanr
[sequence]
RDB06447pHIS3K-CFH61c
pUC119
ef1a-c
CFP-FLAG-His6
3'
-
his3+
kanr
[sequence]
RDB06448pHIS3K-GFH1c
pUC119
nmt1
GFP(S65C)-FLAG-His6
3'
-
his3+
kanr
[sequence]
RDB06449pHIS3K-GFH21c
pUC119
tub1
GFP(S65C)-FLAG-His6
3'
-
his3+
kanr
[sequence]
RDB06450pHIS3K-GFH61c
pUC119
ef1a-c
GFP(S65C)-FLAG-His6
3'
-
his3+
kanr
[sequence]
RDB06451pHIS3K-HFC1c
pUC119
nmt1
His6-FLAG-CFP
5'
-
his3+
kanr
[sequence]
RDB06452pHIS3K-HFC21c
pUC119
tub1
His6-FLAG-CFP
5'
-
his3+
kanr
[sequence]
RDB06453pHIS3K-HFF1c
pUC119
nmt1
His6-FLAG2
5'
-
his3+
kanr
[sequence]
RDB06454pHIS3K-HFG1c
pUC119
nmt1
His6-FLAG-GFP(S65C)
5'
-
his3+
kanr
[sequence]
RDB06441pARG1U-FFH1c
pUC119
nmt1
FLAG2-His6
3'
-
arg1+
ura4+
[sequence]
RDB06442pARG1U-GFH1c
pUC119
nmt1
GFP(S65C)-FLAG-His6
3'
-
arg1+
ura4+
[sequence]
RDB06443pARG1U-HFF1c
pUC119
nmt1
His6-FLAG2
5'
-
arg1+
ura4+
[sequence]
RDB06444pARG1U-HFG1c
pUC119
nmt1
His6-FLAG-GFP(S65C)
5'
-
arg1+
ura4+
[sequence]
RDB06437pARG1K-FFH1c
pUC119
nmt1
FLAG2-His6
3'
-
arg1+
kanr
[sequence]
RDB06438pARG1K-GFH1c
pUC119
nmt1
GFP(S65C)-FLAG-His6
3'
-
arg1+
kanr
[sequence]
RDB06439pARG1K-HFF1c
pUC119
nmt1
His6-FLAG2
5'
-
arg1+
kanr
[sequence]
RDB06440pARG1K-HFG1c
pUC119
nmt1
His6-FLAG-GFP(S65C)
5'
-
arg1+
kanr
[sequence]
RDB06480pARG1KF-CFH1c
pUC119
nmt1
CFP-FLAG-His6
3'
ura4+
arg1+
kanr
[sequence]

* Medium strength nmt1 promoter, ** Low strength nmt1 promoter

S. pombe expression vectors

Reference

  • Matsuyama A, Shirai A, Yashiroda Y, Kamata A, Horinouchi S, Yoshida M. pDUAL, a multipurpose, multicopy vector capable of chromosomal integration in fission yeast. Yeast. 2004 Nov;21(15):1289-305.

  • Matsuyama A, Shirai A, Yoshida M. A series of promoters for constitutive expression of heterologous genes in fission yeast. Yeast 2008 May;25(5):371-6.

  • Matsuyama A, Shirai A, Yoshida M. A novel series of vectors for chromosomal integration in fission yeast. Biochem Biophys Res Commun. 2008 Sep 19;374(2): 315-9.

pDUAL series

Vector sequence is abailable at Chemical Genetics Laboratory.

pDUAL series (Gateway-incompatible)

Cat. num.
Plasmid name
Backbone
Tag
Tag
position
Promoter
Multicopy marker
Integration marker

RDB06178pDUAL
(pUC119)
-
-
-
ura4+
leu1+
[sequence]
RDB06528pDUAL-S
(pUC119)
-
-
-
ura4+
leu1+
[sequence]
RDB06529pDUAL-Pef1a
pDUAL
-
-
ef1a-c
ura4+
leu1+
[sequence]
RDB06530pDUAL-Ptub1
pDUAL
-
-
tub1
ura4+
leu1+
[sequence]
RDB06179pDUAL-HFF1
pDUAL
His6-FLAG2
5'
nmt1
ura4+
leu1+
[sequence]
RDB06531pDUAL-HFF21
pDUAL
His6-FLAG2
5'
tub1
ura4+
leu1+
[sequence]
RDB06532pDUAL-HFF31
pDUAL
His6-FLAG2
5'
cam1
ura4+
leu1+
[sequence]
RDB06180pDUAL-HFF41
pDUAL
His6-FLAG2
5'
nmt1*
ura4+
leu1+
[sequence]
RDB06533pDUAL-HFF51
pDUAL
His6-FLAG2
5'
tif51
ura4+
leu1+
[sequence]
RDB06534pDUAL-HFF61
pDUAL
His6-FLAG2
5'
ef1a-c
ura4+
leu1+
[sequence]
RDB06181pDUAL-HFF81
pDUAL
His6-FLAG2
5'
nmt1**
ura4+
leu1+
[sequence]
RDB06182pDUAL-HFG1
pDUAL
His6-FLAG-GFP(S65C)
5'
nmt1
ura4+
leu1+
[sequence]
RDB06539pDUAL-HFG21
pDUAL
His6-FLAG-GFP(S65C)
5'
tub1
ura4+
leu1+
[sequence]
RDB06540pDUAL-HFG31
pDUAL
His6-FLAG-GFP(S65C)
5'
cam1
ura4+
leu1+
[sequence]
RDB06183pDUAL-HFG41
pDUAL
His6-FLAG-GFP(S65C)
5'
nmt1*
ura4+
leu1+
[sequence]
RDB06541pDUAL-HFG51
pDUAL
His6-FLAG-GFP(S65C)
5'
tif51
ura4+
leu1+
[sequence]
RDB06542pDUAL-HFG61
pDUAL
His6-FLAG-GFP(S65C)
5'
ef1a-c
ura4+
leu1+
[sequence]
RDB06184pDUAL-HFG81
pDUAL
His6-FLAG-GFP(S65C)
5'
nmt1**
ura4+
leu1+
[sequence]
RDB06185pDUAL-FFH1
pDUAL
FLAG2-His6
3'
nmt1
ura4+
leu1+
[sequence]
RDB06535pDUAL-FFH21
pDUAL
FLAG2-His6
3'
tub1
ura4+
leu1+
[sequence]
RDB06536pDUAL-FFH31
pDUAL
FLAG2-His6
3'
cam1
ura4+
leu1+
[sequence]
RDB06186pDUAL-FFH41
pDUAL
FLAG2-His6
3'
nmt1*
ura4+
leu1+
[sequence]
RDB06537pDUAL-FFH51
pDUAL
FLAG2-His6
3'
tif51
ura4+
leu1+
[sequence]
RDB06538pDUAL-FFH61
pDUAL
FLAG2-His6
3'
ef1a-c
ura4+
leu1+
[sequence]
RDB06187pDUAL-FFH81
pDUAL
FLAG2-His6
3'
nmt1**
ura4+
leu1+
[sequence]
RDB06188pDUAL-GFH1
pDUAL
GFP(S65C)-FLAG-His6
3'
nmt1
ura4+
leu1+
[sequence]
RDB06543pDUAL-GFH21
pDUAL
GFP(S65C)-FLAG-His6
3'
tub1
ura4+
leu1+
[sequence]
RDB06544pDUAL-GFH31
pDUAL
GFP(S65C)-FLAG-His6
3'
cam1
ura4+
leu1+
[sequence]
RDB06189pDUAL-GFH41
pDUAL
GFP(S65C)-FLAG-His6
3'
nmt1*
ura4+
leu1+
[sequence]
RDB06545pDUAL-GFH51
pDUAL
GFP(S65C)-FLAG-His6
3'
tif51
ura4+
leu1+
[sequence]
RDB06546pDUAL-GFH61
pDUAL
GFP(S65C)-FLAG-His6
3'
ef1a-c
ura4+
leu1+
[sequence]
RDB06190pDUAL-GFH81
pDUAL
GFP(S65C)-FLAG-His6
3'
nmt1**
ura4+
leu1+
[sequence]
RDB06191pDUAL-YFH1
pDUAL
YFP-FLAG-His6
3'
nmt1
ura4+
leu1+
[sequence]

* Medium strength nmt1 promoter, ** Low strength nmt1 promoter

pDUAL2 series

Vector sequence is abailable at Chemical Genetics Laboratory.

pDUAL2 series (Gateway-incompatible)

Cat. num.
Plasmid name
Backbone
Tag
Tag
position
Promoter
Multicopy marker
Integration marker

RDB06192pDUAL2
(pUC119)
-
-
-
ura4+
ura4+, leu1-
[sequence]
RDB06193pDUAL2-HFF1
pDUAL2
His6-FLAG2
5'
nmt1
ura4+
ura4+, leu1-
[sequence]
RDB06547pDUAL2-HFF21
pDUAL2
His6-FLAG2
5'
tub1
ura4+
ura4+, leu1-
[sequence]
RDB06194pDUAL2-HFF41
pDUAL2
His6-FLAG2
5'
nmt1*
ura4+
ura4+, leu1-
[sequence]
RDB06549pDUAL2-HFF61
pDUAL2
His6-FLAG2
5'
ef1a-c
ura4+
ura4+, leu1-
[sequence]
RDB06195pDUAL2-HFF81
pDUAL2
His6-FLAG2
5'
nmt1**
ura4+
ura4+, leu1-
[sequence]
RDB06196pDUAL2-HFG1
pDUAL2
His6-FLAG-GFP(S65C)
5'
nmt1
ura4+
ura4+, leu1-
[sequence]
RDB06197pDUAL2-HFG41
pDUAL2
His6-FLAG-GFP(S65C)
5'
nmt1*
ura4+
ura4+, leu1-
[sequence]
RDB06198pDUAL2-HFG81
pDUAL2
His6-FLAG-GFP(S65C)
5'
nmt1**
ura4+
ura4+, leu1-
[sequence]
RDB06199pDUAL2-FFH1
pDUAL2
FLAG2-His6
3'
nmt1
ura4+
ura4+, leu1-
[sequence]
RDB06548pDUAL2-FFH21
pDUAL2
FLAG2-His6
3'
tub1
ura4+
ura4+, leu1-
[sequence]
RDB06200pDUAL2-FFH41
pDUAL2
FLAG2-His6
3'
nmt1*
ura4+
ura4+, leu1-
[sequence]
RDB06550pDUAL2-FFH61
pDUAL2
FLAG2-His6
3'
ef1a-c
ura4+
ura4+, leu1-
[sequence]
RDB06201pDUAL2-FFH81
pDUAL2
FLAG2-His6
3'
nmt1**
ura4+
ura4+, leu1-
[sequence]
RDB06202pDUAL2-GFH1
pDUAL2
GFP(S65C)-FLAG-His6
3'
nmt1
ura4+
ura4+, leu1-
[sequence]
RDB06203pDUAL2-GFH41
pDUAL2
GFP(S65C)-FLAG-His6
3'
nmt1*
ura4+
ura4+, leu1-
[sequence]
RDB06204pDUAL2-GFH81
pDUAL2
GFP(S65C)-FLAG-His6
3'
nmt1**
ura4+
ura4+, leu1-
[sequence]
RDB06551pDUAL2-CFH61
pDUAL2
CFP-FLAG-His6
3'
ef1a-c
ura4+
ura4+, leu1-
[sequence]

* Medium strength nmt1 promoter, ** Low strength nmt1 promoter

pREP series

Vector sequence is abailable at Chemical Genetics Laboratory.

pREP series (Gateway-incompatible)

Cat. num.
Plasmid name
Backbone
Tag
Tag
position
Promoter
Selection marker

RDB06580pHFF1
pREP1
His6-FLAG2
5'
nmt1
LEU2
[sequence]
RDB06581pHFF41
pREP41
His6-FLAG2
5'
nmt1*
LEU2
[sequence]
RDB06582pHFF81
pREP81
His6-FLAG2
5'
nmt1**
LEU2
[sequence]
RDB06592pHFF148
(pREP)
His6-FLAG2
5'
ADH1
LEU2
[sequence]
RDB06583pHFG1
pREP1
His6-FLAG-GFP(S65C)
5'
nmt1
LEU2
[sequence]
RDB06584pHFG41
pREP41
His6-FLAG-GFP(S65C)
5'
nmt1*
LEU2
[sequence]
RDB06585pHFG81
pREP81
His6-FLAG-GFP(S65C)
5'
nmt1**
LEU2
[sequence]
RDB06593pHFG148
(pREP)
His6-FLAG-GFP(S65C)
5'
ADH1
LEU2
[sequence]
RDB06586pFFH1
pREP1
FLAG2-His6
3'
nmt1
LEU2
[sequence]
RDB06587pFFH41
pREP41
FLAG2-His6
3'
nmt1*
LEU2
[sequence]
RDB06588pFFH81
pREP81
FLAG2-His6
3'
nmt1**
LEU2
[sequence]
RDB06594pFFH148
(pREP)
FLAG2-His6
3'
ADH1
LEU2
[sequence]
RDB06589pGFH1
pREP1
GFP(S65C)-FLAG-His6
3'
nmt1
LEU2
[sequence]
RDB06590pGFH41
pREP41
GFP(S65C)-FLAG-His6
3'
nmt1*
LEU2
[sequence]
RDB06591pGFH81
pREP81
GFP(S65C)-FLAG-His6
3'
nmt1**
LEU2
[sequence]
RDB06595pGFH148
(pREP)
GFP(S65C)-FLAG-His6
3'
ADH1
LEU2
[sequence]

* Medium strength nmt1 promoter, ** Low strength nmt1 promoter

Integration vectors

Vector sequence is abailable at Chemical Genetics Laboratory.

Gateway-incompatible vectors for chromosomal integration

Cat. num.
Plasmid name
Backbone
Promoter
Multicopy marker
Targeted locus
Positive selection marker

RDB06552pLYS1U
pUC119
-
-
lys1+
ura4+
[sequence]
RDB06567pLYS1U-Pnmt1
pUC119
nmt1
-
lys1+
ura4+
[sequence]
RDB06566pLYS1U-Ptub1
pUC119
tub1
-
lys1+
ura4+
[sequence]
RDB06553pLYS1K
pUC119
-
-
lys1+
kanr
[sequence]
RDB06554pLYS1H
pUC119
-
-
lys1+
hygr
[sequence]
RDB06555pLYS1B
pUC119
-
-
lys1+
blar
[sequence]
RDB06560pHIS3U
pUC119
-
-
his3+
ura4+
[sequence]
RDB06565pHIS3U-Pnmt1
pUC119
nmt1
-
his3+
ura4+
[sequence]
RDB06561pHIS3K
pUC119
-
-
his3+
kanr
[sequence]
RDB06564pHIS3K-Ptub1
pUC119
tub1
-
his3+
kanr
[sequence]
RDB06562pHIS3H
pUC119
-
-
his3+
hygr
[sequence]
RDB06563pHIS3B
pUC119
-
-
his3+
blar
[sequence]
RDB06556pARG1U
pUC119
-
-
arg1+
ura4+
[sequence]
RDB06557pARG1K
pUC119
-
-
arg1+
kanr
[sequence]
RDB06558pARG1H
pUC119
-
-
arg1+
hygr
[sequence]
RDB06559pARG1B
pUC119
-
-
arg1+
blar
[sequence]
RDB06573pLYS1UF
pUC119
-
ura4+
lys1+
ura4+
[sequence]
RDB06572pLYS1KF
pUC119
-
ura4+
lys1+
kanr
[sequence]
RDB06574pLYS1HF
pUC119
-
ura4+
lys1+
hygr
[sequence]
RDB06575pLYS1BF
pUC119
-
ura4+
lys1+
blar
[sequence]
RDB06569pHIS3UF
pUC119
-
ura4+
his3+
ura4+
[sequence]
RDB06568pHIS3KF
pUC119
-
ura4+
his3+
kanr
[sequence]
RDB06570pHIS3HF
pUC119
-
ura4+
his3+
hygr
[sequence]
RDB06571pHIS3BF
pUC119
-
ura4+
his3+
blar
[sequence]
RDB06577pARG1UF
pUC119
-
ura4+
arg1+
ura4+
[sequence]
RDB06576pARG1KF
pUC119
-
ura4+
arg1+
kanr
[sequence]
RDB06578pARG1HF
pUC119
-
ura4+
arg1+
hygr
[sequence]
RDB06579pARG1BF
pUC119
-
ura4+
arg1+
blar
[sequence]

* Medium strength nmt1 promoter, ** Low strength nmt1 promoter


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