Empty Backbone for Microbial Experiment

• E. coli

Catalog num.	Clone name	Form	Registance (E. coli)			Cloning	Promoter		Tag
			Amp	Tet	Cm		lac	T7	FLAG	His	GST
RDB01911	Lambda GEX5	Phage				Restriction Enzyme
RDB06084	pETEG5	Plasmid				Restriction Enzyme
RDB06211	pT7Tet1	Plasmid				Restriction Enzyme
RDB06212	pT7Tet6	Plasmid				Restriction Enzyme
RDB07041	pETHFFc	Plasmid				Gateway®
RDB07050	pETF	Plasmid				Restriction Enzyme
RDB07051	pT7FCam	Plasmid				Restriction Enzyme

• Yeast

  • Expression Vector Backbone of Saccharomyces cerevisiae

  • S. pombe pDUAL and pDUAL2 series vector

  • Expression Vector Backbone of S. pombe

  
  

• Expression Vector Backbone of Saccharomyces cerevisiae

  Shuttle-vector system for Saccharomyces cerevisiae designed to produce C-terminal-Myc-tagged fusion proteins.
  Myc-tag (EQKLISEEDL) can be recognized by mouse monoclonal anitbody 9E10.
  Depositor: Dr. Hidekatsu Iha
  Reference: Biotechniques, 25, 936-938 (1998)., PMID 9863043.
  

  Catalog no.	Name	Tag	Ori	Marker	Promoter	Size (kb)	Usable sites
  RDB01947	pAMH10	Myc	centromere-type	HIS3	ADH1	4.5	6
  RDB01948	pAMH20	Myc	2micron	HIS3	ADH1	5.3	6
  RDB01949	pAML10	Myc	centromere-type	LEU2	ADH1	5.1	6
  RDB01950	pAML20	Myc	2micron	LEU2	ADH1	5.9	6
  RDB01951	pAMT10	Myc	centromere-type	TRP1	ADH1	4.3	5
  RDB01952	pAMT20	Myc	2micron	TRP1	ADH1	5.1	5
  RDB01953	pAMU10	Myc	centromere-type	URA3	ADH1	4.6	6
  RDB01954	pAMU20	Myc	2micron	URA3	ADH1	5.4	6
  RDB01955	pGMH10	Myc	centromere-type	HIS3	GAL1-10	4.8	6
  RDB01956	pGMH20	Myc	2micron	HIS3	GAL1-10	5.6	6
  RDB01957	pGML10	Myc	centromere-type	LEU2	GAL1-10	5.4	6
  RDB01958	pGML20	Myc	2micron	LEU2	GAL1-10	6.2	6
  RDB01959	pGMT10	Myc	centromere-type	TRP1	GAL1-10	4.6	5
  RDB01960	pGMT20	Myc	2micron	TRP1	GAL1-10	5.4	5
  RDB01961	pGMU10	Myc	centromere-type	URA3	GAL1-10	4.9	6
  RDB01962	pGMU20	Myc	2micron	URA3	GAL1-10	5.7	6

  ---

  Empty expression vector backbone of S. cerevisiae to express GFP-tagged protein.
  Depositor: Dr. Hiroshi Takagi
  Reference: Genes Cells, 18 (6): 459-475 (2013)., PMID 23517290.
  

  Catalog no.	Name	Running title
  RDB08380	pRS416-PADH1-ccdB-yEGFP-TTEF1	Empty expression vector backbone of S. cerevisiae.Gateway(R) destination vector.Contains ccdB and use DB3.1 or an equivalent host bacteria.
  RDB08386	pRS416-PADH1-yEGFP-TTEF1	Empty expression vector backbone of S. cerevisiae

  
  

List of S. pombe Vector Series

See also http://www.riken.jp/SPD/Plasmids.html

See also NCBI VECTORS

S. pombe expression vectors (Gateway compatible)

Reference

• Matsuyama A, Shirai A, Yashiroda Y, Kamata A, Horinouchi S, Yoshida M. pDUAL, a multipurpose, multicopy vector capable of chromosomal integration in fission yeast. Yeast. 2004 Nov;21(15):1289-305.

• Matsuyama A, Arai R, Yashiroda Y, Shirai A, Kamata A, Sekido S, Kobayashi Y, Hashimoto A, Hamamoto M, Hiraoka Y, Horinouchi S, Yoshida M. ORFeome cloning and global analysis of protein localization in the fission yeast Schizosaccharomyces pombe. Nature Biotechnol. 2006 Jul; 24(7):841-7.

• Matsuyama A, Shirai A, Yoshida M. A series of promoters for constitutive expression of heterologous genes in fission yeast. Yeast 2008 May;25(5):371-6.

• Matsuyama A, Shirai A, Yoshida M. A novel series of vectors for chromosomal integration in fission yeast. Biochem Biophys Res Commun. 2008 Sep 19;374(2): 315-9.

pDUAL series

Vector sequence is abailable at .

pDUAL series (Gateway-compatible)

Cat. num.	Plasmid name	Backbone	Tag	Tag position	Promoter	Multicopy marker	Integration marker
RDB06130	pDUAL-HFF1c	pDUAL	His6-FLAG2	5'	nmt1	ura4+	leu1+	[sequence]
RDB06485	pDUAL-HFF21c	pDUAL	His6-FLAG2	5'	tub1	ura4+	leu1+	[sequence]
RDB06486	pDUAL-HFF31c	pDUAL	His6-FLAG2	5'	cam1	ura4+	leu1+	[sequence]
RDB06131	pDUAL-HFF41c	pDUAL	His6-FLAG2	5'	nmt1*	ura4+	leu1+	[sequence]
RDB06487	pDUAL-HFF51c	pDUAL	His6-FLAG2	5'	tif51	ura4+	leu1+	[sequence]
RDB06488	pDUAL-HFF61c	pDUAL	His6-FLAG2	5'	ef1a-c	ura4+	leu1+	[sequence]
RDB06132	pDUAL-HFF81c	pDUAL	His6-FLAG2	5'	nmt1**	ura4+	leu1+	[sequence]
RDB06133	pDUAL-HFG1c	pDUAL	His6-FLAG-GFP(S65C)	5'	nmt1	ura4+	leu1+	[sequence]
RDB06493	pDUAL-HFG21c	pDUAL	His6-FLAG-GFP(S65C)	5'	tub1	ura4+	leu1+	[sequence]
RDB06494	pDUAL-HFG31c	pDUAL	His6-FLAG-GFP(S65C)	5'	cam1	ura4+	leu1+	[sequence]
RDB06134	pDUAL-HFG41c	pDUAL	His6-FLAG-GFP(S65C)	5'	nmt1*	ura4+	leu1+	[sequence]
RDB06495	pDUAL-HFG51c	pDUAL	His6-FLAG-GFP(S65C)	5'	tif51	ura4+	leu1+	[sequence]
RDB06496	pDUAL-HFG61c	pDUAL	His6-FLAG-GFP(S65C)	5'	ef1a-c	ura4+	leu1+	[sequence]
RDB06135	pDUAL-HFG81c	pDUAL	His6-FLAG-GFP(S65C)	5'	nmt1**	ura4+	leu1+	[sequence]
RDB06136	pDUAL-HFC1c	pDUAL	His6-FLAG-CFP	5'	nmt1	ura4+	leu1+	[sequence]
RDB06137	pDUAL-HFC41c	pDUAL	His6-FLAG-CFP	5'	nmt1*	ura4+	leu1+	[sequence]
RDB06138	pDUAL-HFC81c	pDUAL	His6-FLAG-CFP	5'	nmt1**	ura4+	leu1+	[sequence]
RDB06139	pDUAL-HFY1c	pDUAL	His6-FLAG-YFP	5'	nmt1	ura4+	leu1+	[sequence]
RDB06140	pDUAL-HFY41c	pDUAL	His6-FLAG-YFP	5'	nmt1*	ura4+	leu1+	[sequence]
RDB06141	pDUAL-HFY81c	pDUAL	His6-FLAG-YFP	5'	nmt1**	ura4+	leu1+	[sequence]
RDB06142	pDUAL-FFH1c	pDUAL	FLAG2-His6	3'	nmt1	ura4+	leu1+	[sequence]
RDB06489	pDUAL-FFH21c	pDUAL	FLAG2-His6	3'	tub1	ura4+	leu1+	[sequence]
RDB06490	pDUAL-FFH31c	pDUAL	FLAG2-His6	3'	cam1	ura4+	leu1+	[sequence]
RDB06143	pDUAL-FFH41c	pDUAL	FLAG2-His6	3'	nmt1*	ura4+	leu1+	[sequence]
RDB06491	pDUAL-FFH51c	pDUAL	FLAG2-His6	3'	tif51	ura4+	leu1+	[sequence]
RDB06492	pDUAL-FFH61c	pDUAL	FLAG2-His6	3'	ef1a-c	ura4+	leu1+	[sequence]
RDB06144	pDUAL-FFH81c	pDUAL	FLAG2-His6	3'	nmt1**	ura4+	leu1+	[sequence]
RDB06145	pDUAL-GFH1c	pDUAL	GFP(S65C)-FLAG-His6	3'	nmt1	ura4+	leu1+	[sequence]
RDB06497	pDUAL-GFH21c	pDUAL	GFP(S65C)-FLAG-His6	3'	tub1	ura4+	leu1+	[sequence]
RDB06498	pDUAL-GFH31c	pDUAL	GFP(S65C)-FLAG-His6	3'	cam1	ura4+	leu1+	[sequence]
RDB06146	pDUAL-GFH41c	pDUAL	GFP(S65C)-FLAG-His6	3'	nmt1*	ura4+	leu1+	[sequence]
RDB06499	pDUAL-GFH51c	pDUAL	GFP(S65C)-FLAG-His6	3'	tif51	ura4+	leu1+	[sequence]
RDB06500	pDUAL-GFH61c	pDUAL	GFP(S65C)-FLAG-His6	3'	ef1a-c	ura4+	leu1+	[sequence]
RDB06147	pDUAL-GFH81c	pDUAL	GFP(S65C)-FLAG-His6	3'	nmt1**	ura4+	leu1+	[sequence]
RDB06148	pDUAL-CFH1c	pDUAL	CFP-FLAG-His6	3'	nmt1	ura4+	leu1+	[sequence]
RDB06149	pDUAL-CFH41c	pDUAL	CFP-FLAG-His6	3'	nmt1*	ura4+	leu1+	[sequence]
RDB06150	pDUAL-CFH81c	pDUAL	CFP-FLAG-His6	3'	nmt1**	ura4+	leu1+	[sequence]
RDB06151	pDUAL-YFH1c	pDUAL	YFP-FLAG-His6	3'	nmt1	ura4+	leu1+	[sequence]
RDB06152	pDUAL-YFH41c	pDUAL	YFP-FLAG-His6	3'	nmt1*	ura4+	leu1+	[sequence]
RDB06153	pDUAL-YFH81c	pDUAL	YFP-FLAG-His6	3'	nmt1**	ura4+	leu1+	[sequence]
RDB06481	pDUAL-FFH1c rfA	pDUAL	FLAG2-His6	3'	nmt1	ura4+	leu1+	[sequence]
RDB06482	pDUAL-FFH1c rfC	pDUAL	FLAG2-His6	3'	nmt1	ura4+	leu1+	[sequence]
RDB06483	pDUAL-YFH1c rfA	pDUAL	YFP-FLAG-His6	3'	nmt1	ura4+	leu1+	[sequence]
RDB06484	pDUAL-YFH1c rfC	pDUAL	YFP-FLAG-His6	3'	nmt1	ura4+	leu1+	[sequence]

* Medium strength nmt1 promoter, ** Low strength nmt1 promoter

pDUAL2 series

Vector sequence is abailable at .

pDUAL2 series (Gateway-compatible)

Cat. num.	Plasmid name	Backbone	Tag	Tag position	Promoter	Multicopy marker	Integration marker
RDB06154	pDUAL2-HFF1c	pDUAL2	His6-FLAG2	5'	nmt1	ura4+	ura4+, leu1-	[sequence]
RDB06155	pDUAL2-HFF41c	pDUAL2	His6-FLAG2	5'	nmt1*	ura4+	ura4+, leu1-	[sequence]
RDB06156	pDUAL2-HFF81c	pDUAL2	His6-FLAG2	5'	nmt1**	ura4+	ura4+, leu1-	[sequence]
RDB06157	pDUAL2-HFG1c	pDUAL2	His6-FLAG-GFP(S65C)	5'	nmt1	ura4+	ura4+, leu1-	[sequence]
RDB06158	pDUAL2-HFG41c	pDUAL2	His6-FLAG-GFP(S65C)	5'	nmt1*	ura4+	ura4+, leu1-	[sequence]
RDB06159	pDUAL2-HFG81c	pDUAL2	His6-FLAG-GFP(S65C)	5'	nmt1**	ura4+	ura4+, leu1-	[sequence]
RDB06160	pDUAL2-HFC1c	pDUAL2	His6-FLAG-CFP	5'	nmt1	ura4+	ura4+, leu1-	[sequence]
RDB06161	pDUAL2-HFC41c	pDUAL2	His6-FLAG-CFP	5'	nmt1*	ura4+	ura4+, leu1-	[sequence]
RDB06162	pDUAL2-HFC81c	pDUAL2	His6-FLAG-CFP	5'	nmt1**	ura4+	ura4+, leu1-	[sequence]
RDB06163	pDUAL2-HFY1c	pDUAL2	His6-FLAG-YFP	5'	nmt1	ura4+	ura4+, leu1-	[sequence]
RDB06164	pDUAL2-HFY41c	pDUAL2	His6-FLAG-YFP	5'	nmt1*	ura4+	ura4+, leu1-	[sequence]
RDB06165	pDUAL2-HFY81c	pDUAL2	His6-FLAG-YFP	5'	nmt1**	ura4+	ura4+, leu1-	[sequence]
RDB06166	pDUAL2-FFH1c	pDUAL2	FLAG2-His6	3'	nmt1	ura4+	ura4+, leu1-	[sequence]
RDB06167	pDUAL2-FFH41c	pDUAL2	FLAG2-His6	3'	nmt1*	ura4+	ura4+, leu1-	[sequence]
RDB06168	pDUAL2-FFH81c	pDUAL2	FLAG2-His6	3'	nmt1**	ura4+	ura4+, leu1-	[sequence]
RDB06169	pDUAL2-GFH1c	pDUAL2	GFP(S65C)-FLAG-His6	3'	nmt1	ura4+	ura4+, leu1-	[sequence]
RDB06170	pDUAL2-GFH41c	pDUAL2	GFP(S65C)-FLAG-His6	3'	nmt1*	ura4+	ura4+, leu1-	[sequence]
RDB06171	pDUAL2-GFH81c	pDUAL2	GFP(S65C)-FLAG-His6	3'	nmt1**	ura4+	ura4+, leu1-	[sequence]
RDB06172	pDUAL2-CFH1c	pDUAL2	CFP-FLAG-His6	3'	nmt1	ura4+	ura4+, leu1-	[sequence]
RDB06173	pDUAL2-CFH41c	pDUAL2	CFP-FLAG-His6	3'	nmt1*	ura4+	ura4+, leu1-	[sequence]
RDB06174	pDUAL2-CFH81c	pDUAL2	CFP-FLAG-His6	3'	nmt1**	ura4+	ura4+, leu1-	[sequence]
RDB06175	pDUAL2-YFH1c	pDUAL2	YFP-FLAG-His6	3'	nmt1	ura4+	ura4+, leu1-	[sequence]
RDB06503	pDUAL2-YFH21c	pDUAL2	YFP-FLAG-His6	3'	nmt1	ura4+	ura4+, leu1-	[sequence]
RDB06176	pDUAL2-YFH41c	pDUAL2	YFP-FLAG-His6	3'	nmt1*	ura4+	ura4+, leu1-	[sequence]
RDB06177	pDUAL2-YFH81c	pDUAL2	YFP-FLAG-His6	3'	nmt1**	ura4+	ura4+, leu1-	[sequence]
RDB06501	pDUAL2-BFH41c	pDUAL2	BFP-FLAG-His6	3'	nmt1*	ura4+	ura4+, leu1-	[sequence]
RDB06502	pDUAL2-BFH81c	pDUAL2	BFP-FLAG-His6	3'	nmt1**	ura4+	ura4+, leu1-	[sequence]

* Medium strength nmt1 promoter, ** Low strength nmt1 promoter

pREP series

Vector sequence is abailable at .

pREP series (Gateway-compatible)

Cat. num.	Plasmid name	Backbone	Tag	Tag position	Promoter	Selection marker
RDB06519	pREP1-ccdB2	pREP1	-	-	nmt1	LEU2	[sequence]
RDB06520	pREP41-ccdB2	pREP41	-	-	nmt1*	LEU2	[sequence]
RDB06521	pREP81-ccdB2	pREP81	-	-	nmt1**	LEU2	[sequence]
RDB06522	pHFF1-ccdB2	pREP1	His6-FLAG2	5'	nmt1	LEU2	[sequence]
RDB06523	pHFF41-ccdB2	pREP41	His6-FLAG2	5'	nmt1*	LEU2	[sequence]
RDB06524	pHFF81-ccdB2	pREP81	His6-FLAG2	5'	nmt1**	LEU2	[sequence]
RDB06525	pHFG1-ccdB2	pREP1	His6-FLAG-GFP(S65C)	5'	nmt1	LEU2	[sequence]
RDB06526	pHFG41-ccdB2	pREP41	His6-FLAG-GFP(S65C)	5'	nmt1*	LEU2	[sequence]
RDB06527	pHFG81-ccdB2	pREP81	His6-FLAG-GFP(S65C)	5'	nmt1**	LEU2	[sequence]
RDB06506	pFFH1-ccdB2	pREP1	FLAG2-His6	3'	nmt1	LEU2	[sequence]
RDB06507	pFFH41-ccdB2	pREP41	FLAG2-His6	3'	nmt1*	LEU2	[sequence]
RDB06508	pFFH81-ccdB2	pREP81	FLAG2-His6	3'	nmt1**	LEU2	[sequence]
RDB06509	pGFH1-ccdB2	pREP1	GFP(S65C)-FLAG-His6	3'	nmt1	LEU2	[sequence]
RDB06510	pGFH41-ccdB2	pREP41	GFP(S65C)-FLAG-His6	3'	nmt1*	LEU2	[sequence]
RDB06511	pGFH81-ccdB2	pREP81	GFP(S65C)-FLAG-His6	3'	nmt1**	LEU2	[sequence]
RDB06513	pHFY1-ccdB2	pREP1	His6-FLAG-YFP	5'	nmt1	LEU2	[sequence]
RDB06512	pHFC1-ccdB2	pREP1	His6-FLAG-CFP	5'	nmt1	LEU2	[sequence]
RDB06515	pYFH1-ccdB2	pREP1	YFP-FLAG-His6	3'	nmt1	LEU2	[sequence]
RDB06505	pYFH1-ccdB2 rfA	pREP1	YFP-FLAG-His6	3'	nmt1	LEU2	[sequence]
RDB06504	pYFH1-ccdB2 rfC	pREP1	YFP-FLAG-His6	3'	nmt1	LEU2	[sequence]
RDB06514	pCFH1-ccdB2	pREP1	CFP-FLAG-His6	3'	nmt1	LEU2	[sequence]

* Medium strength nmt1 promoter, ** Low strength nmt1 promoter

Integration vectors

Vector sequence is abailable at .

Gateway-compatible vectors for chromosomal integration

Cat. num.	Plasmid name	Backbone	Promoter	Tag	Tag position	Multicopy marker	Targeted locus (negative) selection	Positive selection marker
RDB06462	pLYS1U-CFH1c	pUC119	nmt1	CFP-FLAG-His6	3'	-	lys1+	ura4+	[sequence]
RDB06463	pLYS1U-CFH21c	pUC119	tub1	CFP-FLAG-His6	3'	-	lys1+	ura4+	[sequence]
RDB06464	pLYS1U-CFH61c	pUC119	ef1a-c	CFP-FLAG-His6	3'	-	lys1+	ura4+	[sequence]
RDB06465	pLYS1U-FFH1c	pUC119	nmt1	FLAG2-His6	3'	-	lys1+	ura4+	[sequence]
RDB06466	pLYS1U-GFH1c	pUC119	nmt1	GFP(S65C)-FLAG-His6	3'	-	lys1+	ura4+	[sequence]
RDB06467	pLYS1U-GFH21c	pUC119	tub1	GFP(S65C)-FLAG-His6	3'	-	lys1+	ura4+	[sequence]
RDB06468	pLYS1U-GFH61c	pUC119	ef1a-c	GFP(S65C)-FLAG-His6	3'	-	lys1+	ura4+	[sequence]
RDB06477	pLYS1U-YFH1c	pUC119	nmt1	YFP-FLAG-His6	3'	-	lys1+	ura4+	[sequence]
RDB06478	pLYS1U-YFH21c	pUC119	tub1	YFP-FLAG-His6	3'	-	lys1+	ura4+	[sequence]
RDB06479	pLYS1U-YFH61c	pUC119	ef1a-c	YFP-FLAG-His6	3'	-	lys1+	ura4+	[sequence]
RDB06469	pLYS1U-HFC1c	pUC119	nmt1	His6-FLAG-CFP	5'	-	lys1+	ura4+	[sequence]
RDB06470	pLYS1U-HFC21c	pUC119	tub1	His6-FLAG-CFP	5'	-	lys1+	ura4+	[sequence]
RDB06471	pLYS1U-HFC61c	pUC119	ef1a-c	His6-FLAG-CFP	5'	-	lys1+	ura4+	[sequence]
RDB06472	pLYS1U-HFF1c	pUC119	nmt1	His6-FLAG2	5'	-	lys1+	ura4+	[sequence]
RDB06473	pLYS1U-HFG1c	pUC119	nmt1	His6-FLAG-GFP(S65C)	5'	-	lys1+	ura4+	[sequence]
RDB06474	pLYS1U-HFY1c	pUC119	nmt1	His6-FLAG-YFP	5'	-	lys1+	ura4+	[sequence]
RDB06475	pLYS1U-HFY21c	pUC119	tub1	His6-FLAG-YFP	5'	-	lys1+	ura4+	[sequence]
RDB06476	pLYS1U-HFY61c	pUC119	ef1a-c	His6-FLAG-YFP	5'	-	lys1+	ura4+	[sequence]
RDB06458	pLYS1K-FFH1c	pUC119	nmt1	FLAG2-His6	3'	-	lys1+	kanr	[sequence]
RDB06459	pLYS1K-GFH1c	pUC119	nmt1	GFP(S65C)-FLAG-His6	3'	-	lys1+	kanr	[sequence]
RDB06460	pLYS1K-HFF1c	pUC119	nmt1	His6-FLAG2	5'	-	lys1+	kanr	[sequence]
RDB06461	pLYS1K-HFG1c	pUC119	nmt1	His6-FLAG-GFP(S65C)	5'	-	lys1+	kanr	[sequence]
RDB06517	pHIS3U-CFH1c	pUC119	nmt1	CFP-FLAG-His6	3'	-	his3+	ura4+	[sequence]
RDB06455	pHIS3U-GFH1c	pUC119	nmt1	GFP(S65C)-FLAG-His6	3'	-	his3+	ura4+	[sequence]
RDB06518	pHIS3U-YFH1c	pUC119	nmt1	YFP-FLAG-His6	3'	-	his3+	ura4+	[sequence]
RDB06456	pHIS3U-HFF1c	pUC119	nmt1	His6-FLAG2	5'	-	his3+	ura4+	[sequence]
RDB06516	pHIS3U-HFC1c	pUC119	nmt1	His6-FLAG-CFP	5'	-	his3+	ura4+	[sequence]
RDB06457	pHIS3U-HFG1c	pUC119	nmt1	His6-FLAG-GFP(S65C)	5'	-	his3+	ura4+	[sequence]
RDB06445	pHIS3K-CFH1c	pUC119	nmt1	CFP-FLAG-His6	3'	-	his3+	kanr	[sequence]
RDB06446	pHIS3K-CFH21c	pUC119	tub1	CFP-FLAG-His6	3'	-	his3+	kanr	[sequence]
RDB06447	pHIS3K-CFH61c	pUC119	ef1a-c	CFP-FLAG-His6	3'	-	his3+	kanr	[sequence]
RDB06448	pHIS3K-GFH1c	pUC119	nmt1	GFP(S65C)-FLAG-His6	3'	-	his3+	kanr	[sequence]
RDB06449	pHIS3K-GFH21c	pUC119	tub1	GFP(S65C)-FLAG-His6	3'	-	his3+	kanr	[sequence]
RDB06450	pHIS3K-GFH61c	pUC119	ef1a-c	GFP(S65C)-FLAG-His6	3'	-	his3+	kanr	[sequence]
RDB06451	pHIS3K-HFC1c	pUC119	nmt1	His6-FLAG-CFP	5'	-	his3+	kanr	[sequence]
RDB06452	pHIS3K-HFC21c	pUC119	tub1	His6-FLAG-CFP	5'	-	his3+	kanr	[sequence]
RDB06453	pHIS3K-HFF1c	pUC119	nmt1	His6-FLAG2	5'	-	his3+	kanr	[sequence]
RDB06454	pHIS3K-HFG1c	pUC119	nmt1	His6-FLAG-GFP(S65C)	5'	-	his3+	kanr	[sequence]
RDB06441	pARG1U-FFH1c	pUC119	nmt1	FLAG2-His6	3'	-	arg1+	ura4+	[sequence]
RDB06442	pARG1U-GFH1c	pUC119	nmt1	GFP(S65C)-FLAG-His6	3'	-	arg1+	ura4+	[sequence]
RDB06443	pARG1U-HFF1c	pUC119	nmt1	His6-FLAG2	5'	-	arg1+	ura4+	[sequence]
RDB06444	pARG1U-HFG1c	pUC119	nmt1	His6-FLAG-GFP(S65C)	5'	-	arg1+	ura4+	[sequence]
RDB06437	pARG1K-FFH1c	pUC119	nmt1	FLAG2-His6	3'	-	arg1+	kanr	[sequence]
RDB06438	pARG1K-GFH1c	pUC119	nmt1	GFP(S65C)-FLAG-His6	3'	-	arg1+	kanr	[sequence]
RDB06439	pARG1K-HFF1c	pUC119	nmt1	His6-FLAG2	5'	-	arg1+	kanr	[sequence]
RDB06440	pARG1K-HFG1c	pUC119	nmt1	His6-FLAG-GFP(S65C)	5'	-	arg1+	kanr	[sequence]
RDB06480	pARG1KF-CFH1c	pUC119	nmt1	CFP-FLAG-His6	3'	ura4+	arg1+	kanr	[sequence]

* Medium strength nmt1 promoter, ** Low strength nmt1 promoter

S. pombe expression vectors

Reference

• Matsuyama A, Shirai A, Yashiroda Y, Kamata A, Horinouchi S, Yoshida M. pDUAL, a multipurpose, multicopy vector capable of chromosomal integration in fission yeast. Yeast. 2004 Nov;21(15):1289-305.

• Matsuyama A, Shirai A, Yoshida M. A series of promoters for constitutive expression of heterologous genes in fission yeast. Yeast 2008 May;25(5):371-6.

• Matsuyama A, Shirai A, Yoshida M. A novel series of vectors for chromosomal integration in fission yeast. Biochem Biophys Res Commun. 2008 Sep 19;374(2): 315-9.

pDUAL series

Vector sequence is abailable at .

pDUAL series (Gateway-incompatible)

Cat. num.	Plasmid name	Backbone	Tag	Tag position	Promoter	Multicopy marker	Integration marker
RDB06178	pDUAL	(pUC119)	-	-	-	ura4+	leu1+	[sequence]
RDB06528	pDUAL-S	(pUC119)	-	-	-	ura4+	leu1+	[sequence]
RDB06529	pDUAL-Pef1a	pDUAL	-	-	ef1a-c	ura4+	leu1+	[sequence]
RDB06530	pDUAL-Ptub1	pDUAL	-	-	tub1	ura4+	leu1+	[sequence]
RDB06179	pDUAL-HFF1	pDUAL	His6-FLAG2	5'	nmt1	ura4+	leu1+	[sequence]
RDB06531	pDUAL-HFF21	pDUAL	His6-FLAG2	5'	tub1	ura4+	leu1+	[sequence]
RDB06532	pDUAL-HFF31	pDUAL	His6-FLAG2	5'	cam1	ura4+	leu1+	[sequence]
RDB06180	pDUAL-HFF41	pDUAL	His6-FLAG2	5'	nmt1*	ura4+	leu1+	[sequence]
RDB06533	pDUAL-HFF51	pDUAL	His6-FLAG2	5'	tif51	ura4+	leu1+	[sequence]
RDB06534	pDUAL-HFF61	pDUAL	His6-FLAG2	5'	ef1a-c	ura4+	leu1+	[sequence]
RDB06181	pDUAL-HFF81	pDUAL	His6-FLAG2	5'	nmt1**	ura4+	leu1+	[sequence]
RDB06182	pDUAL-HFG1	pDUAL	His6-FLAG-GFP(S65C)	5'	nmt1	ura4+	leu1+	[sequence]
RDB06539	pDUAL-HFG21	pDUAL	His6-FLAG-GFP(S65C)	5'	tub1	ura4+	leu1+	[sequence]
RDB06540	pDUAL-HFG31	pDUAL	His6-FLAG-GFP(S65C)	5'	cam1	ura4+	leu1+	[sequence]
RDB06183	pDUAL-HFG41	pDUAL	His6-FLAG-GFP(S65C)	5'	nmt1*	ura4+	leu1+	[sequence]
RDB06541	pDUAL-HFG51	pDUAL	His6-FLAG-GFP(S65C)	5'	tif51	ura4+	leu1+	[sequence]
RDB06542	pDUAL-HFG61	pDUAL	His6-FLAG-GFP(S65C)	5'	ef1a-c	ura4+	leu1+	[sequence]
RDB06184	pDUAL-HFG81	pDUAL	His6-FLAG-GFP(S65C)	5'	nmt1**	ura4+	leu1+	[sequence]
RDB06185	pDUAL-FFH1	pDUAL	FLAG2-His6	3'	nmt1	ura4+	leu1+	[sequence]
RDB06535	pDUAL-FFH21	pDUAL	FLAG2-His6	3'	tub1	ura4+	leu1+	[sequence]
RDB06536	pDUAL-FFH31	pDUAL	FLAG2-His6	3'	cam1	ura4+	leu1+	[sequence]
RDB06186	pDUAL-FFH41	pDUAL	FLAG2-His6	3'	nmt1*	ura4+	leu1+	[sequence]
RDB06537	pDUAL-FFH51	pDUAL	FLAG2-His6	3'	tif51	ura4+	leu1+	[sequence]
RDB06538	pDUAL-FFH61	pDUAL	FLAG2-His6	3'	ef1a-c	ura4+	leu1+	[sequence]
RDB06187	pDUAL-FFH81	pDUAL	FLAG2-His6	3'	nmt1**	ura4+	leu1+	[sequence]
RDB06188	pDUAL-GFH1	pDUAL	GFP(S65C)-FLAG-His6	3'	nmt1	ura4+	leu1+	[sequence]
RDB06543	pDUAL-GFH21	pDUAL	GFP(S65C)-FLAG-His6	3'	tub1	ura4+	leu1+	[sequence]
RDB06544	pDUAL-GFH31	pDUAL	GFP(S65C)-FLAG-His6	3'	cam1	ura4+	leu1+	[sequence]
RDB06189	pDUAL-GFH41	pDUAL	GFP(S65C)-FLAG-His6	3'	nmt1*	ura4+	leu1+	[sequence]
RDB06545	pDUAL-GFH51	pDUAL	GFP(S65C)-FLAG-His6	3'	tif51	ura4+	leu1+	[sequence]
RDB06546	pDUAL-GFH61	pDUAL	GFP(S65C)-FLAG-His6	3'	ef1a-c	ura4+	leu1+	[sequence]
RDB06190	pDUAL-GFH81	pDUAL	GFP(S65C)-FLAG-His6	3'	nmt1**	ura4+	leu1+	[sequence]
RDB06191	pDUAL-YFH1	pDUAL	YFP-FLAG-His6	3'	nmt1	ura4+	leu1+	[sequence]

* Medium strength nmt1 promoter, ** Low strength nmt1 promoter

pDUAL2 series

Vector sequence is abailable at .

pDUAL2 series (Gateway-incompatible)

Cat. num.	Plasmid name	Backbone	Tag	Tag position	Promoter	Multicopy marker	Integration marker
RDB06192	pDUAL2	(pUC119)	-	-	-	ura4+	ura4+, leu1-	[sequence]
RDB06193	pDUAL2-HFF1	pDUAL2	His6-FLAG2	5'	nmt1	ura4+	ura4+, leu1-	[sequence]
RDB06547	pDUAL2-HFF21	pDUAL2	His6-FLAG2	5'	tub1	ura4+	ura4+, leu1-	[sequence]
RDB06194	pDUAL2-HFF41	pDUAL2	His6-FLAG2	5'	nmt1*	ura4+	ura4+, leu1-	[sequence]
RDB06549	pDUAL2-HFF61	pDUAL2	His6-FLAG2	5'	ef1a-c	ura4+	ura4+, leu1-	[sequence]
RDB06195	pDUAL2-HFF81	pDUAL2	His6-FLAG2	5'	nmt1**	ura4+	ura4+, leu1-	[sequence]
RDB06196	pDUAL2-HFG1	pDUAL2	His6-FLAG-GFP(S65C)	5'	nmt1	ura4+	ura4+, leu1-	[sequence]
RDB06197	pDUAL2-HFG41	pDUAL2	His6-FLAG-GFP(S65C)	5'	nmt1*	ura4+	ura4+, leu1-	[sequence]
RDB06198	pDUAL2-HFG81	pDUAL2	His6-FLAG-GFP(S65C)	5'	nmt1**	ura4+	ura4+, leu1-	[sequence]
RDB06199	pDUAL2-FFH1	pDUAL2	FLAG2-His6	3'	nmt1	ura4+	ura4+, leu1-	[sequence]
RDB06548	pDUAL2-FFH21	pDUAL2	FLAG2-His6	3'	tub1	ura4+	ura4+, leu1-	[sequence]
RDB06200	pDUAL2-FFH41	pDUAL2	FLAG2-His6	3'	nmt1*	ura4+	ura4+, leu1-	[sequence]
RDB06550	pDUAL2-FFH61	pDUAL2	FLAG2-His6	3'	ef1a-c	ura4+	ura4+, leu1-	[sequence]
RDB06201	pDUAL2-FFH81	pDUAL2	FLAG2-His6	3'	nmt1**	ura4+	ura4+, leu1-	[sequence]
RDB06202	pDUAL2-GFH1	pDUAL2	GFP(S65C)-FLAG-His6	3'	nmt1	ura4+	ura4+, leu1-	[sequence]
RDB06203	pDUAL2-GFH41	pDUAL2	GFP(S65C)-FLAG-His6	3'	nmt1*	ura4+	ura4+, leu1-	[sequence]
RDB06204	pDUAL2-GFH81	pDUAL2	GFP(S65C)-FLAG-His6	3'	nmt1**	ura4+	ura4+, leu1-	[sequence]
RDB06551	pDUAL2-CFH61	pDUAL2	CFP-FLAG-His6	3'	ef1a-c	ura4+	ura4+, leu1-	[sequence]

* Medium strength nmt1 promoter, ** Low strength nmt1 promoter

pREP series

Vector sequence is abailable at .

pREP series (Gateway-incompatible)

Cat. num.	Plasmid name	Backbone	Tag	Tag position	Promoter	Selection marker
RDB06580	pHFF1	pREP1	His6-FLAG2	5'	nmt1	LEU2	[sequence]
RDB06581	pHFF41	pREP41	His6-FLAG2	5'	nmt1*	LEU2	[sequence]
RDB06582	pHFF81	pREP81	His6-FLAG2	5'	nmt1**	LEU2	[sequence]
RDB06592	pHFF148	(pREP)	His6-FLAG2	5'	ADH1	LEU2	[sequence]
RDB06583	pHFG1	pREP1	His6-FLAG-GFP(S65C)	5'	nmt1	LEU2	[sequence]
RDB06584	pHFG41	pREP41	His6-FLAG-GFP(S65C)	5'	nmt1*	LEU2	[sequence]
RDB06585	pHFG81	pREP81	His6-FLAG-GFP(S65C)	5'	nmt1**	LEU2	[sequence]
RDB06593	pHFG148	(pREP)	His6-FLAG-GFP(S65C)	5'	ADH1	LEU2	[sequence]
RDB06586	pFFH1	pREP1	FLAG2-His6	3'	nmt1	LEU2	[sequence]
RDB06587	pFFH41	pREP41	FLAG2-His6	3'	nmt1*	LEU2	[sequence]
RDB06588	pFFH81	pREP81	FLAG2-His6	3'	nmt1**	LEU2	[sequence]
RDB06594	pFFH148	(pREP)	FLAG2-His6	3'	ADH1	LEU2	[sequence]
RDB06589	pGFH1	pREP1	GFP(S65C)-FLAG-His6	3'	nmt1	LEU2	[sequence]
RDB06590	pGFH41	pREP41	GFP(S65C)-FLAG-His6	3'	nmt1*	LEU2	[sequence]
RDB06591	pGFH81	pREP81	GFP(S65C)-FLAG-His6	3'	nmt1**	LEU2	[sequence]
RDB06595	pGFH148	(pREP)	GFP(S65C)-FLAG-His6	3'	ADH1	LEU2	[sequence]

* Medium strength nmt1 promoter, ** Low strength nmt1 promoter

Integration vectors

Vector sequence is abailable at .

Gateway-incompatible vectors for chromosomal integration

Cat. num.	Plasmid name	Backbone	Promoter	Multicopy marker	Targeted locus	Positive selection marker
RDB06552	pLYS1U	pUC119	-	-	lys1+	ura4+	[sequence]
RDB06567	pLYS1U-Pnmt1	pUC119	nmt1	-	lys1+	ura4+	[sequence]
RDB06566	pLYS1U-Ptub1	pUC119	tub1	-	lys1+	ura4+	[sequence]
RDB06553	pLYS1K	pUC119	-	-	lys1+	kanr	[sequence]
RDB06554	pLYS1H	pUC119	-	-	lys1+	hygr	[sequence]
RDB06555	pLYS1B	pUC119	-	-	lys1+	blar	[sequence]
RDB06560	pHIS3U	pUC119	-	-	his3+	ura4+	[sequence]
RDB06565	pHIS3U-Pnmt1	pUC119	nmt1	-	his3+	ura4+	[sequence]
RDB06561	pHIS3K	pUC119	-	-	his3+	kanr	[sequence]
RDB06564	pHIS3K-Ptub1	pUC119	tub1	-	his3+	kanr	[sequence]
RDB06562	pHIS3H	pUC119	-	-	his3+	hygr	[sequence]
RDB06563	pHIS3B	pUC119	-	-	his3+	blar	[sequence]
RDB06556	pARG1U	pUC119	-	-	arg1+	ura4+	[sequence]
RDB06557	pARG1K	pUC119	-	-	arg1+	kanr	[sequence]
RDB06558	pARG1H	pUC119	-	-	arg1+	hygr	[sequence]
RDB06559	pARG1B	pUC119	-	-	arg1+	blar	[sequence]
RDB06573	pLYS1UF	pUC119	-	ura4+	lys1+	ura4+	[sequence]
RDB06572	pLYS1KF	pUC119	-	ura4+	lys1+	kanr	[sequence]
RDB06574	pLYS1HF	pUC119	-	ura4+	lys1+	hygr	[sequence]
RDB06575	pLYS1BF	pUC119	-	ura4+	lys1+	blar	[sequence]
RDB06569	pHIS3UF	pUC119	-	ura4+	his3+	ura4+	[sequence]
RDB06568	pHIS3KF	pUC119	-	ura4+	his3+	kanr	[sequence]
RDB06570	pHIS3HF	pUC119	-	ura4+	his3+	hygr	[sequence]
RDB06571	pHIS3BF	pUC119	-	ura4+	his3+	blar	[sequence]
RDB06577	pARG1UF	pUC119	-	ura4+	arg1+	ura4+	[sequence]
RDB06576	pARG1KF	pUC119	-	ura4+	arg1+	kanr	[sequence]
RDB06578	pARG1HF	pUC119	-	ura4+	arg1+	hygr	[sequence]
RDB06579	pARG1BF	pUC119	-	ura4+	arg1+	blar	[sequence]

* Medium strength nmt1 promoter, ** Low strength nmt1 promoter

Expression Vector Backbone of Schizosaccharomyces pombe

Depositor: Dr. Toshiaki Isobe
Reference: PLoS One 9, e112488 (2014)., PMID 25401760.

Catalog No.	plasmid	backborne	Sp marker	Ec marker	insert	remarks
RDB08041	pCtrlki-kanMX6	bacterial	kanMX6	Ap	MCS1-opal stop codon-kanMX6-MCS2	Plasmid vector to constract C-terminal truncated allele by the integration of opal stop codon into target gene using homologous recombination.
RDB08042	pCtFEM3ki-kanMX6	bacterial	kanMX6	Ap	MCS1-Flag-tev-3myc-opal stop codon-kanMX6-MCS2	Plasmid vector to construct C-terminally FEM3 (FLAG-tev-3xmyc) tagged allele of target gene by using homologous recombination.
RDB08045	pCtFLATAki-kanMX6	bacterial	kanMX6	Ap	MCS1-Flag-tev-protein A-opal stop codon-kanMX6-MCS2	Plasmid vector to construct C-terminally FLATA (FLAG-tev-protein A) tagged allele of target gene by using homologous recombination.
RDB08048	pFOX1-CFLATA	replicative	ScLEU2(complement leu1-)	Ap	Ptef-MCS1-Flag-tev-protein A-opal stop codon-Ttef	Plasmid vector to constract replicative expression clone constitutively expressing C-terminally FLATA tag (FLAG-tev-protein A) allele in leu1- strain of S. pombe.
RDB08051	pCtHATAki-kanMX6	bacterial	kanMX6	Ap	MCS1-HA-tev-protein A-opal stop codon-kanMX6-MCS2	Plasmid vector to constract C-terminally HATA (HA-tev-protein A) tagged allele of target gene by using homologous recombination.
RDB08052	pFOX1-CHATA	replicative	ScLEU2(complement leu1-)	Ap	Ptef-MCS1-HA-tev-protein A-opal stop codon-Ttef	Plasmid vector to constract replicative expression clone constitutively expressing C-terminally HATA tag (HA-tev-protein A) allele in leu1- strain of S. pombe.

• Bacillus

RDB00919	pSTE12	Bacillus stearothermophilus shuttle vector
RDB00920	pSTE33	Bacillus stearothermophilus shuttle vector

Gene Disruption

• Thermus thermophilus

Empty Backbone for Mammalian Cell Experiment

• Plasmid/cosmid

Catalog num.	Clone Name	Epitope Tag								Selectable Marker		Cloning		Promoter				Bacterial Resistance
		Flag	HA	2xHA	T7	2xT7	Myc	2xMyc	w/o	Neo	w/o	Restriction Enzyme	Gateway®	CMV	CAG	EF-1	RSV	Amp
RDB05956	pCMV_S-FLAG
RDB06083	pRSV_S-FLAG
RDB07396	pCMV_S-FLAGc
RDB07397	pRSV_s-FLAGc
RDB02136	S-HA-pRc/CMV
RDB02139	D-HA-pRc/CMV
RDB02137	S-T7-pRc/CMV
RDB02138	D-T7-pRc/CMV
RDB02140	S-Myc-pRc/CMV
RDB02141	D-Myc-pRc/CMV
RDB07939	pEF-BOS
RDB07940	pEF-BOS-EX
RDB08938	pCAGGS

RDB	Name of clone	Running title	Class	Seq file
1379	pH2Rneo	Expression vector with simian virus 40 (SV40) early promoter	Vector
1674	pCALNLw	A cassette to introduce cDNA into mammalian chromosome	Vector
1679	pCALwL	A cassette plasmid to insert cDNA into downstream CAG promoter	Vector
1862	pCALNL5	A cassette to introduce cDNA into mammalian chromosome	Vector
1968	pBMSA	Expression vector, inducible promoter	Vector
2546	pxCAG	Expression vector driven by CAG promoter	Vector
3043	pMNSM	Retrovirus expression vector in eukaryotic cells	Vector

pCMV_tag and pRSV_tag assay-ready clones Vectors (ja) Vectors (en) Insert sequences FAQs

Laboratory use only. Not intended for intentional introduction into the environment. RDB Name of clone Running title Class Cloning site Seq file 2136 S-HA-pRc/CMV Expressing HA-tagged fusion protein, CMV promoter Vector RDB2136.pdf RDB2136z.seq 2139 D-HA-pRc/CMV Expressing a fusion protein tagged with duplicated HA, CMV promoter Vector RDB2139.pdf RDB2139z.seq 2137 S-T7-pRc/CMV Expressing T7-tagged fusion protein, CMV promoter Vector RDB2137.pdf RDB2137z.seq 2138 D-T7-pRc/CMV Expressing a fusion protein tagged with duplicated T7, CMV promoter Vector RDB2138.pdf RDB2138z.seq 2140 S-Myc-pRc/CMV Expressing Myc-tagged fusion protein, CMV promoter Vector RDB2140.pdf RDB2140z.seq 2141 D-Myc-pRc/CMV Expressing a fusion protein tagged with duplicated Myc epitopes, CMV promoter Vector RDB2141.pdf RDB2141z.seq Expression vectors listed above are constructed by Dr. Yoshihiro Takemoto of Tokyo Medical and Dental University and published in the DNA Cell Biol. 16 (7): 893-896, 1997. You can select one or two copies of epitope tags among the HA, T7 and Myc. The NotI restriction enzyme site is enable you to create expression vectors tagged your gene of interest with these vectors. After the cloning of your gene, the NotI site also allows inserted gene to be moved to another vectors among these 6 vectors. Please visit the following site for genetic materials deposited to our division by Dr. Takemoto. http://dna.brc.riken.jp/search/dep3357.html go to Head RDB Name of clone Running title Class Cloning site Seq file 6083 pRSV_S-FLAG Expressing FLAG-tagged fusion protein, RSV promoter Vector RDB6083.pdf RDB6083z.seq go to Head RDB Name of clone Running title Class Cloning site Seq file 5956 pCMV_S-FLAG Expressing FLAG-tagged fusion protein, CMV promoter Vector RDB5956.pdf RDB5956z.seq 6072 pCMFlag_lacZ Expression vector of lacZ, with FLAG-tag, control vector DNA clone RDB6072.pdf 6071 pCMFlag_EGFP Expression vector of GFP, with FLAG-tag, control vector DNA clone RDB6072.pdf pCMV_S-FLAG vector is a mammalian expression vector designed to express proteins with FLAG epitope tag (Met-DYKDDDDK). The NotI restriction enzyme site is enable you to create expression vectors tagged your gene of interest. The first ATG of the FLAG tag is included in the NcoI restriction enzyme site (CCATGG) and the NcoI site is available for cloning in-frame the FLAG tag and your interested gene together into bacterial expression vectors. The EcoRI site abutting upon the FLAG tag allows to excite your interested gene with the FLAG tag. PCR products starting with ATG codon can be cloned in-flame into the blunted NotI restriction site. RDB Name of clone Running title Class Cloning site Seq file 7396 pCMV_s-FLAGc Destination vector to generate eukaryotic expression vector, with FLAG-tag Vector RDB7396.pdf RDB7396z.seq pCMV_s-FLAGc vector is a derivative of the pCMV_S-FLAG vector. Gateway® technology offers one-step cloning of your interested gene in Entry clones into the pCMV_s-FLAGc expression vector. Please refer the following website for Entry clones from the Genome Network Project Human Full-Length cDNA. http://dna.brc.riken.jp/en/GNPcloneen.html

Empty Backbone for Recombinant Virus

• Adenovirus

Empty vectors for construction of recombinant adenovirus

RDB no.	Name of vector	Short title	Classification
917	pAxcw	A cassette cosmid for construction of recombinant adenovirus	Vector
918	pAxc	A cassette cosmid for construction of recombinant adenovirus	Vector
1678	pAxCAwt	A cassette cosmid for construction of a recombinant adenovirus	Vector
2564	pAxCEAwtR	Cloning vector to generate recombinant adenovirus driven by human CEA promoter	Vector
2565	pAxCEAwt	Cloning vector to generate recombinant adenovirus driven by human CEA promoter	Vector
3120	pAxcwit	A dual cassette for constructing recombinant adenovirus	Vector
3121	pAxCAwtit	A dual cassette for constructing recombinant adenovirus containing CAG promoter	Vector
3220	pAF16Rct	Cosmid clone of genomic fragment of adenovirus type 5	Vector
3380	pAxCAiLacZit	Shuttle vector to generate recombinant adenovirus harbouring E. coli LacZ	ShuttleVector
3444	pUAd5L	Shuttle vector to generate recombinant adenovirus	Vector
3445	pUAd5LCA	Shuttle vector to generate recombinant adenovirus	Vector
3446	pUAd5LCALNL	Shuttle vector to generate recombinant adenovirus	Vector
3447	pUAd5LCA	Shuttle vector to generate recombinant adenovirus	Vector
3448	pUAd5LCALNL	Shuttle vector to generate recombinant adenovirus	Vector
5212	pAxcwit2	A dual cassette for constructing recombinant adenovirus	Vector
5213	pAxCAwtit2	A dual cassette for constructing recombinant adenovirus	Vector
5214	pAxCALNLwtit2	A dual cassette for constructing recombinant adenovirus	Vector
5215	pAxEFwtit2	A dual cassette for constructing recombinant adenovirus	Vector

Name of vector	Cloning site	Promotera	Cre-loxPb	Production of recombinant adenovirus				RDB no.	Map
				Intact-genome transfectionc	Two-cosmidd	COS-TPCe	Fiber-modified COS-TPC
pAxcw	SwaI					Yes		917	[ pdf ]
pAxCAwt	SwaI	CA				Yes		1678	[ pdf ]
pAxCALNLw	SwaI	CA	Yes			Yes		f
pAxcwit	SwaI			Yes		Yes		3120	[ pdf ]
pAxCAwtit	SwaI	CA		Yes		Yes		3121	[ pdf ]
pUAd5L	SwaI				Yes	Yes	Yes	3444	[ pdf ]
pUAd5LCA	SwaI	CA			Yes	Yes	Yes	3445	[ pdf ]
pUAd5LCALNL	SwaI	CA	Yes		Yes	Yes	Yes	3446	[ pdf ]
pAxCEAwt	SwaI	CEA				Yes		2565	[ pdf ]
pAxcwit2	SwaI			Yesg		Yes		5212	[ pdf ]
pAxCAwtit2	SwaI	CA		Yesg		Yes		5213	[ pdf ]
pAxCALNLwtit2	SwaI	CA	Yes	Yesg		Yes		5214	[ pdf ]
pAxEFwtit2	SwaI	EF-1 alpha		Yesg		Yes		5215	[ pdf ]

a, CA, a modified promoter of the chicken gene for beta-actin with a cytomegalovirus-immediate early enhancer (CMV-IE); CEA, carcinoembryonic antigen; EF-1 alpha, elongation factor 1 alpha.

b, Target gene harbored in the recombinant adenovirus can be expressed by co-infection with AxCANCre (RDB no. 1748, Gene 181: 207-212, 1996).

c, Fukuda et al., Microbiol. Immunol. 50: 643-654, 2006.

d, Use pAF16Rct (RDB no. 3220) together to generate rAd.

e, Miyake et al., Proc. Natl. Acad. Sci. USA 93: 1320-1324, 1996.

f, Available from Takara Bio Inc.

g, PacI restriction enzyme is also applicable for linearization as well as Csp45I restriction enzyme.

Vectors to construct shuttle vector for recombinant adenovirus.
Pdf file is available.

• Retrovirus




RDB no.	Name of vector	Short title	Classification
1699	pRx nZ ires Neo	Nls (nuclear localization signal), lacZ, ires, neo	Vector