pAAV-hSyn-hChR2(H134R)-mCherry plasmid vector腺相关病毒红色荧光表达质粒载体 BioVector NTCC质粒载体菌种细胞基因保藏中心

pAAV-hSyn-hChR2(H134R)-mCherry plasmid vector腺相关病毒红色荧光表达质粒载体

Vector backbonepAAV

Backbone manufacturerInvitrogen
Backbone size w/o insert (bp)4568
Vector typeMammalian Expression, AAV
GROWTH IN BACTERIA
Bacterial Resistance(s)Ampicillin
Growth Temperature37°C
Growth Strain(s)Stbl3
Growth instructionsPlease use Rec A- competent cells such as Stbl3 cells from Invitrogen for transformation.
Copy numberHigh Copy
GENE/INSERT
Gene/Insert namehChR2(H134R)
Alt namehChR2
SpeciesH. sapiens (human)
Insert Size (bp)1647
MutationH134R
Tag / Fusion Protein
mCherry (C terminal on insert)
CLONING INFORMATION
Cloning methodRestriction Enzyme
5′ cloning siteBamHI, SalI, KpnI (not destroyed)
3′ cloning siteEcoRI, HinDIII (not destroyed)
5′ sequencing primerccacgcgaggcgcgagatag
3′ sequencing primerGCAATAGCATGATACAAAGG

Supplier来源:BioVector NTCC Inc.
TEL电话:400-800-2947
Website网址: http://www.biovector.net