H9 [derivative of HuT 78] cell line细胞株 BioVector NTCC质粒载体菌种细胞基因保藏中心

H9 [derivative of HuT 78] cell line细胞株

H9 [derivative of HuT 78]
Cat# BioVector-HTB-176

Product category
Human cells
Organism
Homo sapiens, human
Cell type
cutaneous T lymphocyte
Morphology
lymphoblast
Disease
Lymphoma
Applications
3D cell culture
Immunology

General
Specific applications
This cell line is a suitable transfection host.

Characteristics
Growth properties
Suspension
Derivation
The H9 cell line is a clonal derivative of the Hut 78 cell line (see ATCC TIB-161).
Age
53 years
Ethnicity
White
Gender
Male
Clinical data
The H9 clone was selected for permissiveness for HIV-1 replication, and has been used to isolate and propagate HIV-1 from the blood of patients with acquired immunodeficiency syndrome (AIDS) and pre-AIDS conditions.
Karyotype
This is a near triploid cell line (modal number = 69; range = 58 to 74). The frequency of higher ploidies is 2.5%. The line has an extremely complex karyotype with nearly 60% of the chromosomes in each cell being structurally altered marker chromosomes., Among the markers are t(3p4q), t(5q6q), t(5p6p), i(18q), i(18p); t(4q7p), and del(7)(q32). The first four of these are usually paired. Normal N4, N5, N6, N7, N10, N13, N18, N19, N20 and X are absent.

Tumorigenic
Yes;
Yes, in nude mice inoculated subcutaneously with 10(7) cells
Antigen expression
CD4; HLA A1, B62, C3, DR4, DQ3
Virus susceptibility
Human immunodeficiency virus 1
Genes expressed
interleukin-2 (interleukin 2, IL-2); CD4; HLA: (A1; B62; C3; DR4; DQ3)
Expression markers
Interleukin 2 (IL-2)
Isoenzymes
AK-1, 0
ES-D, 1
G6PD, B
GLO-I, 1
Me-2, 0
PGM1, 1
PGM3, 0
Comments
The H9 clone was selected for permissiveness for HIV-1 replication, and has been used to isolate and propagate HIV-1 from the blood of patients with acquired immunodeficiency syndrome (AIDS) and pre-AIDS conditions.

Handling information
Unpacking and storage instructions
Check all containers for leakage or breakage.
Remove the frozen cells from the dry ice packaging and immediately place the cells at a temperature below _x001f_-130°C, preferably in liquid nitrogen vapor, until ready for use.
Complete medium
The base medium for this cell line is ATCC-formulated RPMI-1640 Medium, ATCC 30-2001. To make the complete growth medium, add the following components to the base medium: fetal bovine serum (ATCC 30-2020) to a final concentration of 10%.
Temperature
37°C
Handling procedure
To insure the highest level of viability, thaw the vial and initiate the culture as soon as possible upon receipt. If upon arrival, continued storage of the frozen culture is necessary, it should be stored in liquid nitrogen vapor phase and not at -70°C.  Storage at -70°C will result in loss of viability.
Thaw the vial by gentle agitation in a 37°C water bath. To reduce the possibility of contamination, keep the O-ring and cap out of the water.  Thawing should be rapid (approximately 2 minutes).
Remove the vial from the water bath as soon as the contents are thawed, and decontaminate by dipping in or spraying with 70% ethanol. All of the operations from this point on should be carried out under strict aseptic conditions.
Transfer the vial contents to a 75 cm2 tissue culture flask and dilute with the recommended complete culture medium (see the specific batch information for the recommended dilution ratio).   It is important to avoid excessive alkalinity of the medium during recovery of the cells.  It is suggested that, prior to the addition of the vial contents, the culture vessel containing the growth medium be placed into the incubator for at least 15 minutes to allow the medium to reach its normal pH (7.0 to 7.6).
Incubate the culture at 37°C in a suitable incubator.  A 5% CO2 in air atmosphere is recommended if using the medium described on this product sheet.
If it is desired that the cryoprotective agent be removed immediately, or that a more concentrated cell suspension be obtained, centrifuge the cell suspension at approximately 125 x g for 5 to 10 minutes.  Discard the supernatant and resuspend the cells with fresh growth medium at the dilution ratio recommended in the specific batch  information.

Subculturing procedure
Cultures can be maintained by addition of fresh medium or replacement of medium. Alternatively, cultures can be established by centrifugation with subsequent resuspension in fresh medium at 5 x 105 viable cells/mL.
Maintain cultures at cell concentrations between 5 x 105 and 2 x 106 viable cells/mL.
Do not allow cell concentration to exceed 3 X 106 cells/mL.
Medium Renewal: Every 2 to 4 days
Reagents for cryopreservation
Culture medium, 95%; DMSO, 5%

Quality control specifications
Mycoplasma contamination
Not detected
STR profiling
Amelogenin: X,Y
CSF1PO: 11
D13S317: 8,12
D16S539: 11,12
D5S818: 11
D7S820: 8,11
THO1: 8,9
TPOX: 8,9
vWA: 14,15

Supplier来源:BioVector NTCC Inc.
TEL电话:400-800-2947
Website网址: http://www.biovector.net