DA6.147 cell line小鼠杂交瘤细胞株 BioVector NTCC质粒载体菌种细胞基因保藏中心

DA6.147 cell line小鼠杂交瘤细胞株

Cell Line Name: DA6.147

Reactivity: Anti- MHC class II alpha chain, human
Antibody Isotype: IgG1
Fusion Species: Mouse x Mouse Hybridoma
Myeloma: NS1
Immunological Donor: BALB/c mouse spleen
Immunogen: Daudi Burkitt lymphoma cells
Transfected DNA: Not Applicable
Cell Line Description: The antibody is directed against the alpha chain of human MHC class II antigens. The antibody is poorly reactive with cell surface MHC antigens, but shows excellent reactivity against alpha chains by Western blotting.
Citation Guidance: If use of this culture results in a scientific publication, it should be cited in the publication as: DA6.147 (ECACC 90110605)
Cell Type: Hybridoma
Morphology: Spherical
Growth Mode: Suspension
Sub Culture Routine: Maintain cultures between 3-9x100,000 cells/ml; 5% CO2; 37°C.
When recovering hybridoma cultures from frozen it is not unusual for growth to be slower than expected initially and there may be an observed decrease in viability. Establishment of an actively proliferating culture may take up to 2 weeks.
On resuscitation a centrifugation step to remove the cryoprotectant is essential. Rapidly thaw the frozen ampoule in a water bath at 37°C for 1-2 minutes. Transfer the contents to a centrifuge tube and slowly add 5-10ml of prewarmed growth media+. Remove a sample for counting. Centrifuge at 100 x g for 2-3 minutes to pellet cells and seed at a relatively high density of 5-7 x 10⁵ cells/ml. Place culture flask flat and observe regularly until viable proliferating cells are seen.
Often hybridoma cultures may benefit from being resuspended with media supplemented with 20% FBS in the early critical stage of culture establishment immediately post resuscitation. Once the culture is established in culture the FBS can be reduced to 10%.
Culture Medium: RPMI 1640 + 2mM glutamine + 10% Foetal Bovine Serum (FBS)

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