CBS7435 strain (aox1-) (缺失AOX1)毕赤酵母突变菌株-BioVector NTCC质粒载体菌株细胞蛋白抗体基因保藏中心

Growth rates of the P. pastoris wild-type strain CBS7435 and knock-out strains aox1, aox1 arg4, his4, aox1 his4, ku70, ku70 gut1, das1, das1 das2 and the corresponding complemented arg4, his4 and gut1 mutant strains were defined by measuring optical density (OD595) of triplicate cultures during exponential growth phase. An overnight culture was inoculated with a single colony in corresponding media and diluted to a starting OD of ∼0.15. The main cultures were grown in standard conditions using 50 ml of buffered minimal media BMD2% (D-glucose), BMG1% (glycerol) or BMM0.5% (methanol) in 250 ml baffled shake flasks (28°C, 120 rpm with 50mm amplitude). Amino acids were added to 40 µg/ml (histidine) or 50 µg/ml (arginine) as required for non-complemented auxotrophic strains.

BioVector质粒载体菌株细胞蛋白抗体基因保藏中心

NTCC典型培养物保藏中心

电话：400-800-2947

Email：Biovector@163.com

企业QQ/微信：1843439339

网址http://www.biovector.net