pKC1139-Cas9 plasmid链霉菌基因编辑质粒CRISPR-Cas9载体 BioVector NTCC质粒载体菌种细胞基因保藏中心

pKC1139-Cas9 plasmid链霉菌基因编辑质粒CRISPR-Cas9载体

Streptomyces expression of codon-optimized Cas9 and custom gRNA

Bacterial Resistance(s)Apramycin
Growth Temperature37°C
Growth Strain(s)DH5alpha
Copy number High Copy

Gene/Insert name sSpCas9
Alt nameStreptomyces codon-optimized Cas9
SpeciesSynthetic
Insert Size (bp)4107
PromoterrpsL(XC)-BbsI

Gene/Insert name gRNA cassette
Species Synthetic
Insert Size (bp)538
MutationBbsI-flanked lacZ cassette inserted in place of spacer
Promotergapdhp(EL)

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