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Wilms’ Tumor 17.94 cell line人肾母细胞瘤细胞株 BioVector NTCC质粒载体菌种细胞基因保藏中心

  • 价  格:¥98965
  • 货  号:Wilms’ Tumor 17.94
  • 产  地:北京
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Wilms’ Tumor 17.94 cell line人肾母细胞瘤细胞株

Cell Line Name: Wilms' Tumor 17.94 cell line人肾母细胞瘤细胞株

Keywords: Wilms' Tumor, cell line, anaplastic. P53.
Cell Line Description: The Wilms' Tumor 17.94 cell line is an anaplastic Wilms' Tumor (WT) cell line, which expressed NCAM, SALL1, and phenotypic features expected of metanephric blastema-derived cells. In Brown et al., (2005) treatment of 17.94 cells with 12-O-Tetradecanoylphorbol 13-acetate caused morphological changes, which led to reduced NCAM and SALL1 expression, but expression of vimentin was maintained. Wilms' Tumor 17.94 cell line contains a TP53 mutation but lacks mutations in WT1, WTX, or CTNNB1, other genes involved in WT pathogenesis.
Species: Human
Tissue of Origin: Kidney
Disease Status: Cancer
Donor Sex: Female
Donor Age: Infant
CellType: Epithelial
Morphology: Epithelial
Growth Mode: Adherent
DNA Profile: DNA Profile (STR Analysis):
Amelogenin: X X
CSF 1PO: 11,12
D13S317: 8, 8
D16S539: 11,11
D18S51:14, 17
D21S11: 29, 29
D3S1358: 16, 16
D5S818: 11, 11
D7S820: 9, 11
D8S1179: 10, 13
FGA: 22, 23
Penta D: 9, 11
Penta E: 7, 11
THO1: 8, 9.3
TPOX: 8, 8
vWA: 14, 16

Karyotype: The cells demonstrate no loss of heterozygosity at 7p, 11p, or 16q although DNA hypermethylation was detected at several loci, including the H19 differentially methylated region and at the PCDH@ gene clusters at 5q31.
Hazard Group (ACDP): 2
Biosafety Information: Unless specified otherwise, at ECACC we routinely handle all of our cell lines at containment level 2 in accordance with the ACDP guidelines (Advisory Committee on Dangerous Pathogens) (UK). All cell cultures have the potential to carry as yet unidentified adventitious agents. It is the responsibility of the end user to ensure that their facilities comply with biosafety regulations for their own country.
ACDP Guidance: Biological agents: Managing the risks in laboratories and healthcare premises.

Subculture Routine: Subculture semi-confluent cultures 1: 2 once a week using TrypLE Express. Seed between 2-4 x 104 cells/cm2. Culture at 37°C with 5% CO2. Freeze medium: 70% medium, 20% FBS, 10% DMSO
Culture Medium: Dulbecco's Modified Eagles Medium, high glucose (DMEM) (80%) with 20% heat - inactivated FBS

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