OCI-AML4 cell line人急性髓系白血病细胞株 BioVector NTCC质粒载体菌种细胞基因保藏中心
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- 货 号:OCI-AML4
- 产 地:北京
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OCI-AML4 cell line人急性髓系白血病细胞株
Cell line: OCI-AML4
NTCC no.: NTCC-ACC729
Species validation: human (Homo sapiens)
Cell type: acute myeloid leukemia
Origin: established in 1987 from the blast cells of the peripheral blood of a 35-year-old woman with acute myeloblastic leukemia (AML M4), secondary to the treatment of nodular sclerosing Hodgkin disease with radiation and chemotherapy; cells were described in the literature to grow slowly with stimulation but responds to IL-3, GM-CSF and mast cell growth factor (MGF); cells exhibit wild-type TP53
NTCC Cell Culture Data:
Morphology: round to polymorphic cells growing singly or in clumps in suspension, some cells are adherent;
Images:
Medium: 80% alpha-MEM (with ribo- and deoxyribonucleosides) + 20% h.i. FBS
Subculture: seed out at ca. 0.5 x 106 cells/ml; maintain at ca. 0.5-1.5 x 106 cells/ml; optimal split ratio 1:2 to 1:4 every 2-4 days
Incubation: at 37 °C with 5% CO2
Doubling time: ca. 24-30 hours
Harvest: maximum density at ca. 2 x 106 cells/ml
Storage: frozen with 70% medium, 20% FBS, 10% DMSO
NTCC Scientific Data:
Mycoplasma: negative in microbiological culture, PCR assays
Immunology: CD3 -, CD4 +, CD13 +, CD14 -, CD15 -, CD33 +, HLA-DR +;
Images:
Authenticity: STR analysis according to the global standard ANSI/ATCC ASN-0002.1-2021 (2021) resulted in an authentic STR profile of the reference STR database.
Cytogenetics: human flat moded near diploid karyotype with 3% polyploidy; -46(43-47)<2n>XX, der(6)(3qter->3q27::3q26->3q25::6p21->6q27::3q26->3q27::3q26->3qter), der(11)t(11;19)(q23;p13.3), der(16)t(1;16)(q22;p13), der(18)dup(18)(q11q23.2)t(X;18)(p22;q23.3), der(19)t(8;19)(q12;p13.1), der(22)t(11;22)(q23;q22)t(11;19)(q23;p13.3); carries semi-cryptic t(11;19) effecting rearrangement of KMT2A (MLL) with MLLT1 (ENL)
Viruses: PCR: EBV -, HBV -, HCV -, HIV-1 -, HIV-2 -, HTLV-I/-II -, MLV -
Supplier来源:BioVector NTCC Inc.
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