plko.1-gfp-puro plasmid vector慢病毒shRNA基因干扰荧光表达质粒载体 BioVector NTCC质粒载体菌种细胞基因保藏中心

plko.1-gfp-puro plasmid vector慢病毒shRNA基因干扰荧光表达质粒载体

RNA interference (RNAi) is a powerful gene-specific
silencing mechanism in mammalian cells. The
BioVector NTCC Inc. pLKO
product line is a viral-vector-based RNAi
library against annotated mouse and human genes.
shRNAs that are processed into siRNAs intracellularly
are delivered by amphotrophic lentivirus particles,
allowing screening in a wide range of mammalian cell
types. In these cells, pLKO shRNA clones permit
rapid, cost-efficient loss-of-function and genetic
interaction screens.
Target cell lines may be transfected with the purified
plasmid for transient or stable gene silencing
(puromycin selection). In addition, self-inactivating
replication incompetent viral particles can be produced
in packaging cells (HEK293T) by co-transfection with
compatible packaging plasmids, found in pLKO
Lentiviral Packaging Mix, Catalog Number SHP001.1,2
Unlike murine-based MMLV or MSCV retroviral
systems, lentiviral-based particles permit efficient
infection and integration of the specific shRNA
construct into differentiated and non-dividing cells,
such as neurons and dendritic cells,3
overcoming low
transfection and integration difficulties associated with
these cell types.

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TEL电话:400-800-2947
Website网址: http://www.biovector.net