X-33, GS115, GS190 Pichia pastoris strain毕赤酵母菌株 BioVector NTCC质粒载体菌种细胞基因保藏中心

X-33, GS115, GS190 Pichia pastoris strain毕赤酵母菌株

Pichia pastoris is an established protein expression host mainly applied for the production of biopharmaceuticals and industrial enzymes. This methylotrophic yeast is a distinguished production system for its growth to very high cell densities, for the available strong and tightly regulated promoters, and for the options to produce gram amounts of recombinant protein per litre of culture both intracellularly and in secretory fashion. However, not every protein of interest is produced in or secreted by P. pastoris to such high titres. Frequently, protein yields are clearly lower, particularly if complex proteins are expressed that are hetero-oligomers, membrane-attached or prone to proteolytic degradation. The last few years have been particularly fruitful because of numerous activities in improving the expression of such complex proteins with a focus on either protein engineering or on engineering the protein expression host P. pastoris.

Several auxotrophic strains (e.g., ade1, arg4, his4, ura3, met2), and combinations thereof are available together with vectors harbouring the respective genes as selectable markers . Auxotrophic strains have been useful for in vivo labelling of proteins.


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