BL21(DE3) ΔserB srain大肠杆菌磷酸丝氨酸磷酸酶敲除株 BioVector NTCC质粒载体菌种细胞基因保藏中心

BL21(DE3) ΔserB srain大肠杆菌磷酸丝氨酸磷酸酶敲除株

用于O-磷酸-L-丝氨酸引入蛋白的大肠杆菌磷酸丝氨酸磷酸酶敲除株，共翻译非天然氨基酸引入蛋白.
BioVectorTM BL21(λDE3) ΔserB is an engineered strain for specific co-translational O-phospho-L-serine incorporation in a protein by an Escherichia coli. To prevent possible enzymatic dephosphorylation of O-phospho-L-serine (Sep) in vivo, the gene encoding phosphoserine phosphatase (serB), which catalyzes the last step in serine biosynthesis, was deleted from Escherichia coli strain BioVectorTMBL21(DE3).

E. coli genotype: fhuA2 [lon] ompT gal (λ DE3) [dcm] ∆hsdS ΔserB
[λ DE3 = λ sBamHIo ∆EcoRI-B int::(lacI::PlacUV5::T7 gene1) i21 ∆nin5]

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