PU6::unc-119_sgRNA C.elegans gene editing plasmid线虫基因编辑质粒载体 BioVector NTCC质粒载体菌种细胞基因保藏中心

PU6::unc-119_sgRNA C.elegans gene editing plasmid线虫基因编辑质粒载体

gRNA target sequence GAATTTTCTGAAATTAAAGA

Vector backbone pUC57
Backbone size w/o insert (bp)2641
Total vector size (bp)3481
Vector typeCRISPR
GROWTH IN BACTERIA
Bacterial Resistance(s)Ampicillin, 100 μg/mL
Growth Temperature37°C
Growth Strain(s)DH5alpha
Copy numberHigh Copy
GENE/INSERT
Gene/Insert nameunc-119 targeting sgRNA
Alt nameunc-119
SpeciesSynthetic
Insert Size (bp)840
Entrez Geneunc-119 (a.k.a. CELE_M142.1)
PromoterC. elegans U6 snRNA pol III promoter
CLONING INFORMATION
Cloning methodRestriction Enzyme
5′ cloning siteEcoRI (not destroyed)
3′ cloning siteHindIII (not destroyed)
5′ sequencing primerM13 (-40) forward
3′ sequencing primerM13 (-48) reverse

Supplier来源:BioVector NTCC Inc.
TEL电话:400-800-2947
Website网址: http://www.biovector.net