pGTMV plasmid vector植物烟草花叶病毒双元表达质粒载体 BioVector NTCC质粒载体菌种细胞基因保藏中心

pGTMV plasmid vector植物烟草花叶病毒双元表达质粒载体

Binary plasmid with a tobacco mosaic virus infectious clone
Vector backbone pCLEAN-G181
(Search Vector Database)
Total vector size (bp)9669
Vector typePlant Expression
GROWTH IN BACTERIA
Bacterial Resistance(s)Kanamycin, 50 μg/mL
Growth Temperature37°C
Growth Strain(s)DH5alpha
Growth instructionsGrow Escherichia coli for 36 h at 37ºC in kanamycin plates. Grow Agrobacterium tumefaciens for 4 days at 28ºC in plates with rifampicin, tetracycline and kanamycin. Note that replication in A. tumefaciens requieres a pSoup-derivative as a helper plasmid.
Copy numberHigh Copy
GENE/INSERT
Gene/Insert nameTobacco mosaic virus
Alt nameTMV
SpeciesTobacco mosaic virus
PromoterCauliflower mosaic virus 35S

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