FLYRD18 cell line人纤维肉瘤细胞株 BioVector NTCC质粒载体菌种细胞基因保藏中心

FLYRD18 cell line人纤维肉瘤细胞株

FLYRD18 has been developed together with FLYA13 (NTCC no. 95091901) for packaging of retroviruses, it is especially useful for in vivo gene delivery. Both cell lines have been derived from HT1080 (NTCC catalogue no. 85111505). FLYRD18 has been transfected with Moloney murine leukaemia virus cores with envelope glycoproteins of cat endogenous virus RD114. This cell line is able to produce human complement-resistant retroviral vectors at titres consistently higher than 1x10,000,000i.u./ml. Addition of 4µg/ml blasticidin S and 10µg/ml phleomycin into the media may enhance the packaging function. No replication competent virus has been detected in this cell line.

Species: Human
Tissue: fibrosarcoma
Growth Properties: Adherent
Morphology: Epithelial-like

Growth Medium: DMEM + 2mM Glutamine + 10% Foetal Bovine Serum (FBS).

Subculturing Procedure: Split sub-confluent cultures (70-80%) 1:20 i.e. seeding at 1x10,000 cells/cm² using 0.25% trypsin or trypsin/EDTA; 5% CO2; 37°C.

Supplier来源:BioVector NTCC Inc.
TEL电话:400-800-2947
Website网址: http://www.biovector.net