pAK405 sphingomonads plasmid vector鞘酯单胞菌基因敲除载体自杀质粒 BioVector NTCC质粒载体菌种细胞基因保藏中心

pAK405 sphingomonads plasmid vector鞘酯单胞菌基因敲除载体自杀质粒

pAK405 plasmid map and schematic of the gene deletion strategy. (A) Plasmid pAK405 has the following features: a pBR322 oriV (suicide plasmid for Sphingomonas), the RP4 oriT (for conjugal transfer between E. coli and Sphingomonas), the pUC18 multiple cloning site in lacZ′ (allowing blue/white screening), nptII (for selection on kanamycin), and rpsL1 (for streptomycin counterselection).

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