pRIV-2 plasmid vector质粒载体 BioVector NTCC质粒载体菌种细胞基因保藏中心

BioVector's bspRIV-2 plasmid vector质粒载体

Riboflavin auxotrophic mutant C. famata 6
10 of IV biochemical group was transformed with
genomic library of this species. The electrotransformed cells were plated on the minimal medium
without leucine and riboflavin. One colony of yeast
prototroph was obtained. The plasmid isolation from
this prototroph was carried out by E. coli retransformation (using electroporation). This plasmid was digested by the restriction endonucleases: SalI, KpnI,
EcoRI, BamHI, SacI, PstI, SphI, HincII. Digestion
analysis showed that this plasmid, designated as
BioVector's pRIV-2, bears insert of size ~11.7 kb. We were able
to determine preliminary location of digestion sites
for several restriction endonucleases, namely BamHI,
KpnI, EcoRI and SacI.
Plasmid pRIV-2 transformed to Leu+ Rib+ phenotype not only strain 610 but also mutant 815.
The electrotransformation frequency in both cases
was approximately 1 × 103
transformants/µg DNA.

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