Mo Y98 strain绵羊肺炎支原体(Mycoplasma ovipneumoniae,MO) BioVector NTCC质粒载体菌种细胞基因保藏中心

Mo Y98 strain绵羊肺炎支原体(Mycoplasma ovipneumoniae,MO)

绵羊肺炎支原体(Mycoplasma ovipneumoniae,MO)是绵羊传染性胸膜肺炎(Contagious ovine pleuropneumo-nia)的主要致病菌。
The BioVector's Y98 standard M. ovipneumoniaestrain was obtained from the National Institutes for Food and Drug Control. Mycoplasma broth base and fetal equine serum were used to culture M. ovipneumoniae.
Mycoplasma ovipneumoniae is a respiratory bacterium associated with economically impactful pneumonia in domestic sheep and goats since 1972, when it was first described.. Although M. ovipneumoniae was previously reported to affect species of Caprinae (sheep, goats, and muskoxen), recent studies have identified the bacterium in animals outside Caprinae.
Maximum likelihood tree showing the phylogenetic position of BioVector's Mycoplasma ovipneumoniae Y98 T within the Hyopneumoniae cluster of Mycoplasmataceae based on 16S rRNA gene sequences.

Product category
Bacteria
Strain designation
NTCC 10151 [Y98]
Type strain
Yes
Genome sequenced strain
Yes
Isolation source
Ovine respiratory tract
Product format
Freeze-dried
Storage conditions
2°C to 8°C

Handling information
Medium
BioVector's Medium 247: PPLO broth without CV (pH 7.8) with horse serum (not inactivated) and yeast extract
Temperature
37°C
Atmosphere
Broth: Aerobic
Plates: Anaerobic
Handling procedure
Follow instructions as suggested for the culturing of Mollicutes:

               PROCEDURES FOR PROPAGATING MOLLICUTES:

Open the vial according to the enclosed instructions.
Using a Pasteur or 1.0 mL pipette, withdraw approximately 0.5 to 1.0 mL from a tube containing 2.5 mL.  Rehydrate the entire pellet.
Aseptically transfer this aliquot back into the tube.  Mix well.
Make serial dilutions by transferring 0.25 mL from the original tube to a tube containing 2.25 mL.  Repeat process by transferring 0.25 mL from the second to a third tube, etc.  Dilutions are important, not only for titration purposes, but also to keep culture in varying stages of growth.  Many strains will die out rapidly once acid or alkaline conditions are reached.  It is recommended to prepare several dilutions from the initial tube as the cryoprotectant used in the freeze_x001e_drying process often inhibits growth.
Use an uninoculated tube of broth to serve as a control.
Plates may be inoculated to check colonial morphology.  You can also spot each dilution on the surface of plate (4 or more/plate) to determine the number of colony-forming units.  However, not all strains do well on solid medium.
Incubate all tubes and plates under the recommended conditions and appropriate temperature.  The time necessary for growth will vary from strain to strain.  Growth on plates generally requires additional incubation.
Depending on the medium used, growth will be indicated by increased turbidity, a color change, or both.
Tubes may be incubated aerobically, but plates are incubated under anaerobic conditions using an anaerobe jar or other appropriate method.  The incubation temperature is 37°C.
Turbidity appears in the first few dilution tubes within 48 to 72 hours.  Additional incubation is required for colonies to appear on solid medium.
Subsequent, fresh transfers grow more rapidly than the original rehydrated culture.  This strain produces good turbidity.
Handling notes
Store vials at freezer temperatures until ready to use.

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