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RBL-1B2 cell line大鼠嗜碱性细胞白血病细胞株
RBL-1B2 Rat basophilic leukaemia granulocyte cell line
Cat# BioVector935916
Cell Line Description: Exhibits marked variations in differentiation when monitored for basophilic granule development, histamine content and membrane receptor sites for IgE.
Species: Rat
Tissue of Origin: blood
CellType: Lymphoblast
Growth Mode: Suspension
Karyotype: Not specified
Subculture Routine: Maintain cultures between 3x100,000 to 1x1,000,000 cells/ml; 5% CO2; 37°C. Treat as suspension when sub-culturing. Some fibroblast-like cells within the population will attach. Please disregard these and subculture as per normal suspension cell lines.
Culture Medium: EMEM (EBSS) + 2mM Glutamine + 1mM Sodium Pyruvate (NaP) + 1% Non Essential Amino Acids (NEAA) + 10-20% Foetal Bovine Serum (FBS).
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