NALM-21 cell line人急性未分化型白血病细胞株 BioVector NTCC质粒载体菌种细胞基因保藏中心

NALM-21 cell line细胞株

Cell line: NALM-21
NTCC no.: NTCC925971
Species validation: human (Homo sapiens)
Cell type: B cell precursor leukemia
Origin: established from a bone marrow specimen of a 63-year-old man with acute undifferentiated leukemia (AUL) at relapse in 1990; cells were described in the literature to carry two Philadelphia chromosomes t(9;22) with a BCR-ABL1 e1-a2 fusion gene; the cell line is a serial sister cell line of NALM-20
 NTCC Cell Culture Data:
Morphology: round cells growing singly and in clusters in suspension;
image:

Medium: 80-90% RPMI 1640 + 10-20% h.i. FBS
Subculture: seed out at ca. 0.5 x 106 cells/ml; maintain at ca. 0.2-2.0 x 106 cells/ml; split saturated culture 1:2 to 1:3 every 2-3 days
Incubation: at 37 °C with 5% CO2
Doubling time: ca. 50 hours
Harvest: maximum densitiy of ca. 3 x 106 cells/ml
Storage: frozen with 70% medium, 20% FBS, 10% DMSO
 NTCC Scientific Data:
Mycoplasma: negative in microbiological culture, PCR assays
Immunology: CD3 -, CD10 +, CD13 +, CD19 +, CD38 +, CD80 -, CD138 -, HLA-DR +, sm/cyIgG -, sm/cyIgM -, sm/cykappa -, sm/cylambda -; image
Authenticity: STR analysis according to the global standard ANSI/ATCC ASN-0002.1-2021 (2021) resulted in an authentic STR profile of the reference STR database.
Cytogenetics: human near-diploid karyotype; 46(44-48)<2n>X, -Y, +8, inv(4)(p14q35), i(8)(q10)x2, t(9;22)(q34;q11); carries t(9;22) effecting BCR-ABL1 rearrangement; resembles published karyotype
Viruses: PCR: EBV -, HBV -, HCV -, HIV-1 -, HIV-2 -, HTLV-I/-II -, MLV -

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