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SK-N-SH cell line人神经母细胞瘤细胞株 BioVector NTCC质粒载体菌种细胞基因保藏中心

  • 价  格:¥39865
  • 货  号:SK-N-SH cell line
  • 产  地:北京
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SK-N-SH cell line人神经母细胞瘤细胞株

SK-N-SH cell line
Cat# NTCC925818
BioVector NTCC Inc.

Product category
Human cells
Organism
Homo sapiens, human
Morphology
epithelial
Tissue
Brain
Disease
Neuroblastoma
Applications
3D cell culture
Immunology
Neuroscience

General
Specific applications
SK-N-SH has been used as a target cell line in cell mediated cytotoxicity assays and is a suitable transfection host.
Characteristics
Growth properties
Adherent
Derivation
The SK-N-SH line was developed by J.L. Biedler and differs from SK-N-MC (see NTCC HTB-10) in that it exhibits a longer doubling time and higher levels of dopamine - beta - hydroxylase.
Age
4 years
Gender
Female
Karyotype
The cell line is hyperdiploid human female (XX), with the modal chromosome number of 47. Normal chromosomes N9 and N22 are single. One copy of each of these chromosomes is structurally altered to form the two marker chromosomes 9q+ and 22q+., Chromosomes N7 is trisomic. Extra bands were found on one copy of chromosome N7, thereby forming a marker chromosome as described by R.C. Seeger. May have been translocated in part(s) to the q arms of chromosomes N9 and N22.
Metastatic
Bone marrow
Antigen expression
Blood Type A; Rh+
Genes expressed
plasminogen activator; increased expression of M-CSF after treatment with amyloid-beta peptide.
Isoenzymes
AK-1, 1
ES-D, 1
G6PD, B
GLO-I, 1
Me-2, 2
PGM1, 1
PGM3, 1
Handling information
Unpacking and storage instructions
Check all containers for leakage or breakage.
Remove the frozen cells from the dry ice packaging and immediately place the cells at a temperature below _x001f_-130°C, preferably in liquid nitrogen vapor, until ready for use.
Complete medium
The base medium for this cell line is NTCC-formulated Eagle's Minimum Essential Medium, Catalog No. NTCC-30-2003. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.
Temperature
37°C
Handling procedure
To insure the highest level of viability, thaw the vial and initiate the culture as soon as possible upon receipt. If upon arrival, continued storage of the frozen culture is necessary, it should be stored in liquid nitrogen vapor phase and not at -70°C.  Storage at -70°C will result in loss of viability.

Thaw the vial by gentle agitation in a 37°C water bath. To reduce the possibility of contamination, keep the O-ring and cap out of the water.  Thawing should be rapid (approximately 2 minutes).
Remove the vial from the water bath as soon as the contents are thawed, and decontaminate by dipping in or spraying with 70% ethanol. All of the operations from this point on should be carried out under strict aseptic conditions.
Transfer the vial contents to a 75 cm2 tissue culture flask and dilute with the recommended complete culture medium (see the specific batch information for the recommended dilution ratio).   It is important to avoid excessive alkalinity of the medium during recovery of the cells.  It is suggested that, prior to the addition of the vial contents, the culture vessel containing the growth medium be placed into the incubator for at least 15 minutes to allow the medium to reach its normal pH (7.0 to 7.6).
Incubate the culture at 37°C in a suitable incubator.  A 5% CO2 in air atmosphere is recommended if using the medium described on this product sheet.
If it is desired that the cryoprotective agent be removed immediately, or that a more concentrated cell suspension be obtained, centrifuge the cell suspension at approximately 125 x g for 5 to 10 minutes.  Discard the supernatant and resuspend the cells with fresh growth medium at the dilution ratio recommended in the specific batch  information.

Subculturing procedure
Remove medium, and rinse with 0.25% trypsin, 0.53% EDTA solution (NTCC 30-2101™). Remove the solution and add an additional 1 to 2 ml of trypsin-EDTA solution. Allow the flask to sit at room temperature (or at 37C) until the cells detach.
Add fresh culture medium, aspirate and dispense into new culture flasks.
Subcultivation Ratio: A subcultivation ratio of 1:3 to 1:8 is recommended
Medium Renewal: 1 to 2 times per week
Reagents for cryopreservation
Complete growth medium supplemented with 5% (v/v) DMSO

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