​ML-2 Human acute myelomonocytic leukemia cell line人急性粒-单核细胞白血病细胞株 BioVector NTCC质粒载体菌种细胞基因保藏中心

ML-2 cell line人急性粒-单核细胞白血病细胞株

Cell line:
ML-2
NTCC no.:
NTCC-ACC 15
Species validation:
human (Homo sapiens)
Cell type:
acute myelomonocytic leukemia
Origin:
established from the peripheral blood of a 26-year-old man with acute myeloid leukemia (AML M4) at diagnosis of AML (following T-non-Hodgkin lymphoma and T-ALL) in 1978; carries t(6;11)(q27;q23) leading to KMT2A-AFDN (MLL-MLLT4; MLL-AF6) fusion gene; sister cell lines are ML-1 and ML-3
Reference(s):
14493, 16799, 14819, 14606
Biosafety level:
1
Permissions and restrictions:
A

NTCC Cell Culture Data:
Morphology:
round, single cells in suspension; image; image; image
Medium:
80-90% RPMI 1640 + 10-20% h.i. FBS
Subculture:
seed out at ca. 0.5 x106 cells/ml; after thawing, culture should first be started with 20% FBS in 24-well-plates. Possibly, cells do not grow during the first few days and the viability may decrease; then, do not dilute, but exchange medium partially carefully. In logarithmic growth phase split 1:3 to 1:6 every 2-3 days; maintain at 0.5-1.2 x 106 cells/ml
Incubation:
at 37 °C with 5% CO2
Doubling time:
ca. 60 hours
Harvest:
maximum density at 1.0-1.5 x 106 cells/ml
Storage:
frozen with 70% medium, 20% FBS, 10% DMSO

NTCC Scientific Data:
Mycoplasma:
initial contamination was eliminated with BM-Cyclin (tiamulin & minocycline), then negative in DAPI, microbiological culture, RNA hybridization and continuously in PCR assays
Immunology:
CD3 -, CD4 -, CD14 -, CD15 +, CD19 -, CD33 +, CD34 -, cyCD68 +; image
Authenticity:
STR analysis according to the global standard ANSI/ATCC ASN-0002.1-2021 (2021) resulted in an authentic STR profile of the reference STR database.
Cytogenetics:
human near tetraploid karyotype - 92(84-94)<4n>XX, -Y, -Y, -7, -9, -10, -10, +11, +12, +12, +13, +13, -15, -16, -17, -17, +18, +18, -20, -20, +4mar, der(1)t(1;?)(p21;?)x2, del(6)(q23)x2, der(6)t(6;11)(q27;?q23)x2, ?der(11)t(6;11)(q27;?q23)/del(11)(q23)x2, der(11)t(11;?)(?->11p11->11q23:)x2, der(11)t(11;?)(q11-13;?), dup(13)(q32->qter)x2, der(18)t(5;?;18)(q21;?;q11)x2 - extensive ch 11 rearrangements (inc. 11q23, typical of M4 and M5 AML) obscure the cytogenetic picture - ML-2 may carry rare t(6;11)
Molec. Genetics:
expression of fusion gene KMT2A-AFDN (MLL-MLLT4; MLL-AF6) confirmed by RT-PCR
Viruses:
ELISA: reverse transcriptase negative; PCR: EBV -, HBV -, HCV -, HHV-8 -, HIV-1 -, HIV-2 -, HTLV-1/2 -, MLV -, SMRV -

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