mutz-8 cell line人急性髓性白血病细胞株 BioVector NTCC质粒载体菌种细胞基因保藏中心

mutz-8 cell line人急性髓性白血病细胞株

Cell line:
MUTZ-8
NTCC no.:
NTCC-93689
Species validation:
human (Homo sapiens)
Cell type:
acute myeloid leukemia
Origin:
established in 1994 from the peripheral blood of a 63-year-old woman with secondary acute myelomonocytic leukemia (sAML M4) 25 years after onset of myelodysplastic syndrom (MDS) and 2 years after chemotherapy with Ara-C; cells were described in the literature to carry JAK2 and TP53 mutations and a deletion of the 5q31 AML/MDS region effected by a t(5;11) translocation and to be constitutively cytokine-dependent
Reference(s):
16047, 16046, 14871
Biosafety level:
1
Permissions and restrictions:
A
Depositor-specific restrictions:
Transfer to third parties needs to be negotiated with the depositor.

NTCC Cell Culture Data:
Morphology:
round to polymorph cells growing singly in suspension; image; image; image; image
Medium:
80% alpha-MEM (with ribo- and deoxyribonucleosides) + 20% h.i. FBS + 10 ng/ml GM-CSF or 10% vol conditioned medium of cell line 5637 (NTCC930035)
Subculture:
seed out at ca. 0.8 x 106 cells/ml in 24-well plates; maintain at ca. 0.5-1.0 x 106 cells/ml; split culture 1:2 once or twice a week; after thawing cells may need some days to recover and a certain amount of cell debris may be allways visible; CAVEAT: by favoring selective growth, suboptimal culture of cytokine-dependent cell lines may promote outgrowth of factor-independent subclones. Selective conditions include cytokine insufficiency and inadequate cell density. Thus, the DSMZ cannot guarantee indefinite stability of factor-dependence of cell lines.
Incubation:
at 37 °C with 5% CO2
Doubling time:
ca. 3-5 days
Harvest:
maximum density at ca. 1.2 x 106 cells/ml
Storage:
frozen with 70% medium, 20% FBS, 10% DMSO

DSMZ Scientific Data:
Mycoplasma:
negative in microbiological culture, PCR assays
Immunology:
CD3 -, CD4 -, CD13 +, CD19 -, CD33 +, CD34 +, HLA-DR +; image
Authenticity:
STR analysis according to the global standard ANSI/ATCC ASN-0002.1-2021 (2021) resulted in an authentic STR profile of the reference STR database.
Cytogenetics:
human flat-moded hypertriploid/hypotetraploid karyotype; 71-92<4n>XXXX, -7, -7, -8, -9, -11, -12, -14, der(5)t(5;11)(q21;q10)x2, der(11;12)(p10;q10), der(17)t(10;17)(q11;q24)?dup(17)(p13pter)x2; matches published karyotype
Molec. Genetics:
expression of mutated JAK2 confirmed by RT-PCR
Viruses:
PCR: EBV -, HBV -, HCV -, HIV-1 -, HIV-2 -, HTLV-I/-II -, MLV -

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