pVL1392-XylE Baculovirus Control Vector杆状病毒系统质粒载体 BioVector NTCC质粒载体菌种细胞基因保藏中心

The Baculovirus Expression Vector System(BEVS) is a convenient and versatileeukaryotic system for heterologous geneexpression. Baculovirus expression providescorrect folding of recombinant protein aswell as disulfide bond formation,oligomerization and other important post_x0002_translational modifications. Consequently,the overexpressed protein exhibits theproper biological activity and function.The Baculovirus Expression Vector Systemis based on the introduction of a foreigngene into a nonessential region of the viralgenome via homologous recombinationwith a transfer vector containing thecloned gene; an event that occurs in theco-transfected insect cells. The production of foreign protein is achieved by infectionof additional insect cell cultures with theresultant recombinant virus. TheBaculovirus Expression Vector Systemfrom BD Biosciences Pharmingen employsa modified Autographa californica nuclearpolyhedrosis virus (AcNPV) genome—BD BaculoGold™ DNA, and anappropriate transfer vector. The diversityof AcNPV-based transfer vectors,combined with available S. frugiperda Sf9and Sf21 cell lines, establish baculovirusexpression as a preferred system forfunctional eukaryotic gene expression andthe large-scale production of recombinantproteins. The baculovirus expression system offersthe following advantages over prokaryoticand other eukaryotic systems:• High Level of Protein ExpressionYields of up to 100 mg of protein per109 cells.• Post-Translational ModificationsIncluding disulfide bond formation,phosphorylation, glycosylation,oligomerization, and folding.• Relevant CellularCompartmentalization of ProteinsSecreted, membrane-bound, cytoplasmicor nuclear.• Capacity of Large cDNA InsertsAccommodates genes up to 8 kb.• Simultaneous Expression of Multiple GenesWith multiple promoter transfer vectors.I