Syn61 E.coli strain大肠杆菌菌株/感受态细胞 BioVector NTCC质粒载体菌种细胞基因保藏中心

Syn61 E.coli strain大肠杆菌菌株/感受态细胞

Recoded E. coli strain without TCG, TCA, or TAG codons in all open reading frames. Full sequence - GenBank: CP040347.1

Bacterial Resistance(s)Streptomycin, 50 μg/mL
Growth Temperature37°C
Growth Strain(s)This is a recoded E.coli strain
Growth instructionsStreptomycin resistant by virtue of K43R mutation in rpsL. Grow in LB or 2xYT, 100 micrograms/mL streptomycin to maintain

Gene/Insert nameRecoded E. coli strain without TCG, TCA, or TAG codons
GenBank IDCP040347.1

The compressed genome of Syn61 was designed by systematically replacing the TCG, TCA, and TAG codons with their synonyms AGC, AGT, and TAA, respectively, in all open reading frames.

Genotyping primers for presence of synthetic insert in 100k01 in Syn61 strain:
GE282 AAAAAGGTCGGGCCGGACGGTC
GE283 GCAATAATGGCAGCCACACCTTG

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