SB-28-Ohlfest mouse glioma cell line小鼠神经胶质瘤细胞株 BioVector NTCC质粒载体菌种细胞基因保藏中心

SB-28-Ohlfest mouse glioma cell line小鼠神经胶质瘤细胞株

Cell line:SB28-Ohlfest
NTCC®no.:ACC 880
Species:murine (mouse) (Mus musculus)
Cell type:glioma
Origin:Established in 2012 from the brain tissue of a neonatal female C57BL/6 mouse in which a glioma was induced by intraventricular transfection of Sleeping Beauty (SB)-flanked mouse PDGF-B, silencing hairpin-RNA for p53 and NRas (V12). The cells express GFP and firefly luciferase. In the brain of syngenic mice, the cells are described to grow invasively and aggressively, and these tumors are poorly immunogenic and resistant to checkpoint inhibition therapy. The cell line was originally named SB28. The addition "Ohlfest" in the name of the cell line is a dedication to brain tumor pioneer Dr. John Ohlfest, who died in 2013; Ohlfest Web
Reference(s):18211, 18212, 18213
Biosafety level:1, GMO-S1
Risk assessment:genetically modified organism, according to German and European legislation allocated into group S1 (Sleeping Beauty Transposon-targeted terminally integration of V12-Nras into the mouse genome)
Permissions and restrictions:A, D
Depositor-specific restrictions:Transfer to third parties needs to be negotiated with the depositor.
DSMZ Cell Culture Data:
Morphology:fibroblastoid cells growing adherently as monolayer; image; image; image; image; image
Medium:90% Dulbecco´s MEM (with 4.5g/l glucose) + 10% h.i. FBS
Subculture:seed out at about 0.5 x 106 cells in a 75 cm2 flask; split 1:4 up to 1:10 every 2-5 days using trypsin/EDTA; optimal cell density 2-3 x 106 cells/75 cm2
Incubation:at 37 °C with 10% CO2
Doubling time:ca. 30 hours
Harvest:cell harvest of ca. 8 x 106 cells/80 cm2 flask
Storage:frozen with 70% medium, 20% FBS, 10% DMSO
NTCC® Scientific Data:
Mycoplasma:negative in PCR assay
Species validation:Species-level identification was carried out by mitochondrial Cytochrome C Oxidase Subunit 1 (COI) DNA barcoding according standard ANSI/ATCC ASN-0003-2015 and revealed Mus musculus species.
Molec. Genetics:expression of firefly luciferase and GFP was confirmed.
Viruses:PCR: MLV +

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