Hep55.1c cell line小鼠肝细胞癌细胞株 BioVector NTCC质粒载体菌种细胞基因保藏中心

Hep55.1c cell line小鼠肝细胞癌细胞株

DescriptionHep-55.1C cells are derived from C57BL/6J mice and offer a valuable resource for studying hepatocellular carcinoma. The cell line can be used to investigate tumor initiation, progression, metastasis, and response to treatments. By studying these cells, researchers can gain insights into the complex biology of hepatocellular carcinoma and contribute to the development of diagnostic techniques and targeted therapies.
OrganismMouse
TissueLiver
DiseaseHepatocellular carcinoma
SynonymsHEP-55.1C, 55.1C
Characteristics
AgeAdult
GenderFemale
MorphologyEpithelial-like
Growth propertiesAdherent
Identifiers / Biosafety / Citation
CitationHep-55.1C (CLS catalog number 400201)
Biosafety level1
Expression / Mutation
Protein expressionKeratin 8, Keratin 18, Vimentin.
TumorigenicYes, in C57BL/6J mice
Mutational profilep53 wt
Handling
Culture MediumDMEM
Medium supplements10% FBS, w: 4.5 g/L Glucose, w: 4 mM L-Glutamine, w: 1.5 g/L NaHCO3, w: 1.0 mM Sodium pyruvate
Passaging solutionAccutase
SubculturingRemove medium and rinse the adherent cells using PBS without calcium and magnesium (3-5 ml PBS for T25, 5-10ml for T75 cell culture flasks). Add Accutase (1-2ml per T25, 2.5ml per T75 cell culture flask), the cell sheet must be covered completely. Incubate at ambient temperature for 8-10 minutes. Carefully resuspend the cells with medium (10 ml), centrifuge for 5 min at 300xg, resuspend cells in fresh medium and dispense into new flasks which contain fresh medium.
Split ratioA ratio of 1:4 to 1:8 is recommended
Fluid renewalEvery 3 to 5 days
Freezing recoveryStart culture from cryovial at a cell density of 3 to 4 x 10^4 cells/cm^2. The cells will recover within 24 to 48 hours.
Freeze mediumCM-1 (CLS catalog number 800100) or CM-ACF (CLS catalog number 806100)
Handling of cryopreserved culturesThe cells come deep-frozen shipped on dry ice. Please make sure that the vial is still frozen. If immediate culturing is not intended, the cryovial must be stored below -150 degree Celsius after arrival. If immediate culturing is intended, please follow the below instructions: Quickly thaw by rapid agitation in a 37 degree Celsius water bath within 40-60 seconds. The water bath should have clean water containing an antimicrobial agent. As soon as the sample has thawed, remove the cryovial from the water bath. A small ice clump should still remain and the vial should still be cold. From now on, all operations should be carried out under aseptic conditions. Transfer the cryovial to a sterile flow cabinet and wipe with 70% alcohol. Carefully open the vial and transfer the cell suspension into a 15 ml centrifuge tube containing 8 ml of culture medium (room temperature). Resuspend the cells carefully. Centrifuge at 300 x g for 3 min and discard the supernatant. The centrifugation step may be omitted, but in this case the remains of the freeze medium have to be removed 24 hours later. Resuspend the cells carefully in 10 ml fresh cell culture medium and transfer them into two T25 cell culture flasks. All further steps are described in the subculture section.
Handling of proliferating culturesOne or two cell culture flasks come filled with cell culture medium. Collect the entire medium in 1 or 2 x 50 ml centrifuge tubes, respectively. Carefully add 5 ml of cell culture medium to each T25 cell culture flask. Control the cell morphology and confluency under the microscope. Incubate at 37 degree Celsius for a minimum of 24 hours. Spin down the collected medium at 300 x g for 3 minutes to collect the cells which may have detached during transit. If a cell pellet is visible, resuspend the cells in 5 ml of cell culture medium and transfer to a T25 cell culture flask. Incubate at 37 degree Celsius for a minimum of 24 hours.
Quality control / Genetic profile / HLA
SterilityMycoplasma contamination was excluded through PCR-based and luminescence-based mycoplasma assays. Bacterial or fungal contaminations are detected through daily visual cell monitoring.
STR profile
M_18-3: 16
M_4-2: 20.3
M_6-7: 17
M_3-2: 14
M_19-2: 13
M_7-1: 26.2/27.2
M_1-1: 16
M_8-1: 16
M_2-1: 15
M_15-3: 22.3
M_6-4: 18
M_11-2: 16
M_1-2: 20
M_17-2: 15
M_12-1: 17
M_5-5: 16/17
M_X-1: 28
M_13-1: 17

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