pBUE411-GGB plasmid vector植物基因编辑质粒载体 BioVector NTCC质粒载体菌种细胞基因保藏中心

pBUE411-GGB plasmid vector植物基因编辑质粒载体

Wheat GRF4-GIF1 chimera and pPLTP::BBM cassette in pBUE411

BACKBONE
Vector backbonepBUE411
Backbone size w/o insert (bp)6500
Total vector size (bp)23361
Vector typePlant Expression, CRISPR ; plant binary vector
Selectable markersBasta
GROWTH IN BACTERIA
Bacterial Resistance(s)Kanamycin, 50 μg/mL
Growth Temperature37°C
Growth Strain(s)DH5alpha
Copy numberHigh Copy
GENE/INSERT 1
Gene/Insert nameGRF4-GIF1 chimera cassette
SpeciesSynthetic; Wheat; Agrobacterium tumefaciens
Insert Size (bp)4234
PromoterUbiquitin
CLONING INFORMATION FOR GENE/INSERT 1
Cloning methodGibson Cloning
5′ sequencing primerctgtcaaacactgatagtttCTGCAGGCGCGCTAATTCC
3′ sequencing primertcgtttcccgccttcagtttGAGCTCGGTACC
(Common Sequencing Primers)
GENE/INSERT 2
Gene/Insert namepPLTP::BBM cassette
SpeciesZea mays; Agrobacterium tumefaciens
Insert Size (bp)3469
Promotermaize PLTP
CLONING INFORMATION FOR GENE/INSERT 2
Cloning methodRestriction Enzyme
5′ cloning siteAscI (not destroyed)
3′ cloning siteSpeI (not destroyed)
5′ sequencing primerGGCGCGCCACGCTGCTACTGCTGCTAC
3′ sequencing primerACTAGTtcggtacccgggGATCTAGT

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