出品公司: | Addgene |
---|---|
载体名称: | pAc5.1B-EGFP |
质粒类型: | 昆虫细胞表达载体 |
高拷贝/低拷贝: | 高拷贝 |
启动子: | -- |
克隆方法: | 多克隆位点,限制性内切酶 |
载体大小: | 6089 bp |
5' 测序引物及序列: | Ac5 forward:ACACAAAGCCGCTCCATCAG |
3' 测序引物及序列: | BGH reverse:TAGAAGGCACAGTCGAGG |
载体标签: | EGFP |
载体抗性: | 氨苄青霉素(Ampicillin) |
筛选标记: | -- |
备注: | 克隆菌株:Dh5 alpha 或 XL1-blue |
产品目录号: | 21181 |
稳定性: | -- |
组成型: | -- |
病毒/非病毒: | -- |
pAc5.1B-EGFP质粒载体图谱序列抗性价格报价Biovector NTCC Inc.现货
- 价 格:¥7920
- 货 号:BiovectorpAc5.1B-EGFP
- 产 地:北京
- BioVector NTCC典型培养物保藏中心
- 联系人:Dr.Xu, Biovector NTCC Inc.
电话:400-800-2947 工作QQ:1843439339 (微信同号)
邮件:Biovector@163.com
手机:18901268599
地址:北京
- 已注册
载体基本信息
载体质粒图谱和多克隆位点信息
载体简介
载体序列
LOCUS pAc5.1B-EGFP 6089 bp DNA SYN DEFINITION pAc5.1B-EGFP ACCESSION KEYWORDS SOURCE ORGANISM other sequences; artificial sequences; vectors. FEATURES Location/Qualifiers source 1..6089 /organism="pAc5.1B-EGFP" /mol_type="other DNA" CDS 726..1454 /label="ORF frame 3" /translation="MMPDHRLISASFAERSACAVVVFWNSFNFRTVRFDFSFLAYSKS KVKPKKRDGNTKCGKTVVEGKGCGAAEGRVGRGVAGSVAGRDVTDVGATPLTMCACVY YSCLATRRLFFSFYLALSSAISYACSTHRMLPCPLCVILARNRQLFKQRLVNENAKIN KSTIWLLIAMCVQPTIATKATTQWLSLFHFLFASACEQDGTTGKRNYADKEQTKFWPK NIKAPDTNAFAITIAIFNIVYEAV*" CDS complement(1940..2416) /label="ORF frame 2" /translation="MLSALKSRSKLLPYMGMNCSPIGFSFTENFLRNWVFVLVCVLYI AMKSVLPWVLSVAACDSIWGLLIFDTSAGYLYAVMVTVLLHIFSPTTHCMPKHTYVHV FIVGLLVMSSDIKTLQLNNIHISLRLLLSALQIGYSGTFVQHTSSSRGITSRRKHT*" misc_feature 2525..2544 /label="AC5_primer" /translation="MLSALKSRSKLLPYMGMNCSPIGFSFTENFLRNWVFVLVCVLYI AMKSVLPWVLSVAACDSIWGLLIFDTSAGYLYAVMVTVLLHIFSPTTHCMPKHTYVHV FIVGLLVMSSDIKTLQLNNIHISLRLLLSALQIGYSGTFVQHTSSSRGITSRRKHT*" CDS 2588..3445 /label="ORF frame 2" /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLT LKFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFK DDGNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKN GIKVNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDH MVLLEFVTAAGITLGMDELYKPSLNSADIQHSGGRSSLEGPRFEGKPIPNPLLGLDST RTGHHHHHH*" gene 2591..3304 /label="EGFP" /gene="EGFP" /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLT LKFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFK DDGNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKN GIKVNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDH MVLLEFVTAAGITLGMDELYKPSLNSADIQHSGGRSSLEGPRFEGKPIPNPLLGLDST RTGHHHHHH*" misc_feature complement(2633..2654) /label="EGFP_N_primer" /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLT LKFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFK DDGNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKN GIKVNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDH MVLLEFVTAAGITLGMDELYKPSLNSADIQHSGGRSSLEGPRFEGKPIPNPLLGLDST RTGHHHHHH*" misc_feature 2771..2800 /label="Y66 (EGFP)" /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLT LKFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFK DDGNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKN GIKVNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDH MVLLEFVTAAGITLGMDELYKPSLNSADIQHSGGRSSLEGPRFEGKPIPNPLLGLDST RTGHHHHHH*" misc_feature 3241..3262 /label="EGFP_C_primer" /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLT LKFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFK DDGNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKN GIKVNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDH MVLLEFVTAAGITLGMDELYKPSLNSADIQHSGGRSSLEGPRFEGKPIPNPLLGLDST RTGHHHHHH*" misc_feature 3374..3415 /label="V5" /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLT LKFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFK DDGNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKN GIKVNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDH MVLLEFVTAAGITLGMDELYKPSLNSADIQHSGGRSSLEGPRFEGKPIPNPLLGLDST RTGHHHHHH*" misc_feature 3425..3442 /label="6xHIS" /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLT LKFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFK DDGNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKN GIKVNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDH MVLLEFVTAAGITLGMDELYKPSLNSADIQHSGGRSSLEGPRFEGKPIPNPLLGLDST RTGHHHHHH*" misc_feature complement(3465..3482) /label="BGH_rev_primer" /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLT LKFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFK DDGNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKN GIKVNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDH MVLLEFVTAAGITLGMDELYKPSLNSADIQHSGGRSSLEGPRFEGKPIPNPLLGLDST RTGHHHHHH*" terminator complement(3525..3644) /label="SV40_PA_terminator" /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLT LKFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFK DDGNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKN GIKVNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDH MVLLEFVTAAGITLGMDELYKPSLNSADIQHSGGRSSLEGPRFEGKPIPNPLLGLDST RTGHHHHHH*" misc_feature complement(3539..3558) /label="EBV_rev_primer" /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLT LKFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFK DDGNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKN GIKVNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDH MVLLEFVTAAGITLGMDELYKPSLNSADIQHSGGRSSLEGPRFEGKPIPNPLLGLDST RTGHHHHHH*" CDS complement(3672..4301) /label="ORF frame 1" /translation="MLVRGAEPMEKRQQRGLFTVPGLLLAGKSRERSLKLSLMVVIYL PGQHGLQRGHPDAAGSEKNHNGEGHPASRRERQQDVAQRVGRHAGDNGLLLAETFGGG TSDEGLSEGVQDSEYRKRQADHRRAPAKAVLAENDPERCRHLSYELHDKEDSHKCGDD SHAPRPPEGADWVEGSQGHRSTDHNQPYHICRGFTCFKKPPTPPPEPET*" CDS 3750..4352 /label="ORF frame 3" /translation="MISRPMPLRAFNPVSSFRWARGMTIVAALMTVFFIMQLVGQVPA ALWVIFGEDRFRWSATMIGLSLAVFGILHALAQAFVTGPATKRFGEKQAIIAGMAADA LGYVLLAFATRGWMAFPIMILLASGGIGMPALQAMLSRQVDDDHQGQLQGSLAALTSQ QKARNRKKAALLAFFHRLRPPDEHHKNRRSSQRWRNPTGL*" rep_origin complement(4256..4875) /label="pBR322_origin" /translation="MISRPMPLRAFNPVSSFRWARGMTIVAALMTVFFIMQLVGQVPA ALWVIFGEDRFRWSATMIGLSLAVFGILHALAQAFVTGPATKRFGEKQAIIAGMAADA LGYVLLAFATRGWMAFPIMILLASGGIGMPALQAMLSRQVDDDHQGQLQGSLAALTSQ QKARNRKKAALLAFFHRLRPPDEHHKNRRSSQRWRNPTGL*" gene complement(5030..5890) /label="Ampicillin" /gene="Ampicillin" /translation="MISRPMPLRAFNPVSSFRWARGMTIVAALMTVFFIMQLVGQVPA ALWVIFGEDRFRWSATMIGLSLAVFGILHALAQAFVTGPATKRFGEKQAIIAGMAADA LGYVLLAFATRGWMAFPIMILLASGGIGMPALQAMLSRQVDDDHQGQLQGSLAALTSQ QKARNRKKAALLAFFHRLRPPDEHHKNRRSSQRWRNPTGL*" CDS complement(5030..5890) /label="ORF frame 2" /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGY IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVE YSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRL DRWEPELNEAIPNDERDTTMPAAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPL LRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIA EIGASLIKHW*" promoter complement(5932..5960) /label="AmpR_promoter" /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGY IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVE YSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRL DRWEPELNEAIPNDERDTTMPAAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPL LRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIA EIGASLIKHW*" ORIGIN 1 cttcaaggaa ttaattctat attctaaaaa cacaaatgat acttctaaaa aaaatcatga 61 atggcatcaa ctctgaatca aatctttgca gatgcaccta cttctcattt ccactgtcac 121 atcatttttc cagatctcgc tgcctgttat gtggcccaca aaccaagaca cgttttatgg 181 ccattaaagc tggctgatcg tcgccaaaca ccaaatacat atcaatatgt acattcgaga 241 aagaagcgat caaagaagcg tcttcgggcg agtaggagaa tgcggaggag aaggagaacg 301 agctgatcta gtatctctcc acaatccaat gccaactgac caactggcca tattcggagc 361 aatttgaagc caatttccat cgcctggcga tcgctccatt cttggctata tgtttttcac 421 cgttcccggg gccattttca aagactcgtc ggtaagataa gattgtgtca ctcgctgtct 481 ctcttcattt gtcgaagaat gctgaggaat ttcgcgatga cgtcggcgag tattttgaag 541 aatgagaata atttgtattt atacgaaaat cagttagtgg aattttctac aaaaacatgt 601 tatctataga taattttgtt gcaaaatatg ttgactatga caaagattgt atgtatatac 661 ctttaatgta ttctcatttt cttatgtatt tataatggca atgatgatac tgatgatatt 721 ttaagatgat gccagaccac aggctgattt ctgcgtcttt tgccgaacgc agtgcatgtg 781 cggttgttgt tttttggaat agtttcaatt ttcggactgt ccgctttgat ttcagtttct 841 tggcttattc aaaaagcaaa gtaaagccaa aaaagcgaga tggcaatacc aaatgcggca 901 aaacggtagt ggaaggaaag gggtgcgggg cagcggaagg aagggtgggg cggggcgtgg 961 cggggtctgt ggctgggcgc gacgtcaccg acgttggagc cactcctttg accatgtgtg 1021 cgtgtgtgta ttattcgtgt ctcgccactc gccggttgtt tttttctttt tatctcgctc 1081 tctctagcgc catctcgtac gcatgctcaa cgcaccgcat gttgccgtgt cctttatgcg 1141 tcattttggc tcgaaatagg caattattta aacaaagatt agtcaacgaa aacgctaaaa 1201 taaataagtc tacaatatgg ttacttattg ccatgtgtgt gcagccaacg atagcaacaa 1261 aagcaacaac acagtggctt tccctctttc actttttgtt tgcaagcgcg tgcgagcaag 1321 acggcacgac cggcaaacgc aattacgctg acaaagagca gacgaagttt tggccgaaaa 1381 acatcaaggc gcctgatacg aatgcatttg caataacaat tgcgatattt aatattgttt 1441 atgaagctgt ttgacttcaa aacacacaaa aaaaaaaata aaacaaatta tttgaaagag 1501 aattaggaat cggacagctt atcgttacgg gctaacagca caccgagacg aaatagctta 1561 cctgacgtca cagcctctgg aagaactgcc gccaagcaga gagagagaga aaaagaggga 1621 gagcagctta gaccgcatgt gcttgtgtgt gaggcgtctc tctcttcgtc tcctgtttgc 1681 gcaaacgcat agactgcact gagaaaatcg attacctatt ttttatgaat gaatatttgc 1741 actattacta ttcaaaacta ttaagatagc aatcacattc aatagccaaa tactatacca 1801 cctgagcgat gcaacgaaat gatcaatttg agcaaaaatg ctgcatattt aggacggcat 1861 cattatagaa atgcttcttg ctgtgtactt ttctctcgtc tggcagctgt ttcgccgtta 1921 ttgttaaaac cggcttaagt taggtgtgtt ttctacgact agtgatgccc ctactagaag 1981 atgtgtgttg cacaaatgtc cctgaataac caatttgaag tgcagatagc agtaaacgta 2041 agctaatatg aatattattt aactgtaatg ttttaatatc gctggacatt actaataaac 2101 ccactataaa cacatgtaca tatgtatgtt ttggcataca atgagtagtt ggggaaaaaa 2161 tgtgtaaaag caccgtgacc atcacagcat aaagataacc agctgaagta tcgaatatga 2221 gtaaccccca aattgaatca catgccgcaa ctgataggac ccatggaagt acactcttca 2281 tggcgatata caagacacac acaagcacga acacccagtt gcggaggaaa ttctccgtaa 2341 atgaaaaccc aatcggcgaa caattcatac ccatatatgg taaaagtttt gaacgcgact 2401 tgagagcgga gagcattgcg gctgataagg ttttagcgct aagcgggctt tataaaacgg 2461 gctgcgggac cagttttcat atcactaccg tttgagttct tgtgctgtgt ggatactcct 2521 cccgacacaa agccgctcca tcagccagca gtcgtctaat ccagagaccc cggatcgggg 2581 taccaacatg gtgagcaagg gcgaggagct gttcaccggg gtggtgccca tcctggtcga 2641 gctggacggc gacgtaaacg gccacaagtt cagcgtgtcc ggcgagggcg agggcgatgc 2701 cacctacggc aagctgaccc tgaagttcat ctgcaccacc ggcaagctgc ccgtgccctg 2761 gcccaccctc gtgaccaccc tgacctacgg cgtgcagtgc ttcagccgct accccgacca 2821 catgaagcag cacgacttct tcaagtccgc catgcccgaa ggctacgtcc aggagcgcac 2881 catcttcttc aaggacgacg gcaactacaa gacccgcgcc gaggtgaagt tcgagggcga 2941 caccctggtg aaccgcatcg agctgaaggg catcgacttc aaggaggacg gcaacatcct 3001 ggggcacaag ctggagtaca actacaacag ccacaacgtc tatatcatgg ccgacaagca 3061 gaagaacggc atcaaggtga acttcaagat ccgccacaac atcgaggacg gcagcgtgca 3121 gctcgccgac cactaccagc agaacacccc catcggcgac ggccccgtgc tgctgcccga 3181 caaccactac ctgagcaccc agtccgccct gagcaaagac cccaacgaga agcgcgatca 3241 catggtcctg ctggagttcg tgaccgccgc cgggatcact ctcggcatgg acgagctgta 3301 caagccaagc ttgaattctg cagatatcca gcacagtggc ggccgctcga gtctagaggg 3361 cccgcggttc gaaggtaagc ctatccctaa ccctctcctc ggtctcgatt ctacgcgtac 3421 cggtcatcat caccatcacc attgagttta aacccgctga tcagcctcga ctgtgccttc 3481 taaggcctga gctcgctgat cagcctcgat cgaggatcca gacatgataa gatacattga 3541 tgagtttgga caaaccacaa ctagaatgca gtgaaaaaaa tgctttattt gtgaaatttg 3601 tgatgctatt gctttatttg taaccattat aagctgcaat aaacaagtta acaacaacaa 3661 ttgcattcat tttatgtttc aggttcaggg ggaggtgtgg gaggtttttt aaagcaagta 3721 aaacctctac aaatgtggta tggctgatta tgatcagtcg accgatgccc ttgagagcct 3781 tcaacccagt cagctccttc cggtgggcgc ggggcatgac tatcgtcgcc gcacttatga 3841 ctgtcttctt tatcatgcaa ctcgtaggac aggtgccggc agcgctctgg gtcattttcg 3901 gcgaggaccg ctttcgctgg agcgcgacga tgatcggcct gtcgcttgcg gtattcggaa 3961 tcttgcacgc cctcgctcaa gccttcgtca ctggtcccgc caccaaacgt ttcggcgaga 4021 agcaggccat tatcgccggc atggcggccg acgcgctggg ctacgtcttg ctggcgttcg 4081 cgacgcgagg ctggatggcc ttccccatta tgattcttct cgcttccggc ggcatcggga 4141 tgcccgcgtt gcaggccatg ctgtccaggc aggtagatga cgaccatcag ggacagcttc 4201 aaggatcgct cgcggctctt accagccagc aaaaggccag gaaccgtaaa aaggccgcgt 4261 tgctggcgtt tttccatagg ctccgccccc ctgacgagca tcacaaaaat cgacgctcaa 4321 gtcagaggtg gcgaaacccg acaggactat aaagatacca ggcgtttccc cctggaagct 4381 ccctcgtgcg ctctcctgtt ccgaccctgc cgcttaccgg atacctgtcc gcctttctcc 4441 cttcgggaag cgtggcgctt tctcaatgct cacgctgtag gtatctcagt tcggtgtagg 4501 tcgttcgctc caagctgggc tgtgtgcacg aaccccccgt tcagcccgac cgctgcgcct 4561 tatccggtaa ctatcgtctt gagtccaacc cggtaagaca cgacttatcg ccactggcag 4621 cagccactgg taacaggatt agcagagcga ggtatgtagg cggtgctaca gagttcttga 4681 agtggtggcc taactacggc tacactagaa ggacagtatt tggtatctgc gctctgctga 4741 agccagttac cttcggaaaa agagttggta gctcttgatc cggcaaacaa accaccgctg 4801 gtagcggtgg tttttttgtt tgcaagcagc agattacgcg cagaaaaaaa ggatctcaag 4861 aagatccttt gatcttttct acggggtctg acgctcagtg gaacgaaaac tcacgttaag 4921 ggattttggt catgagatta tcaaaaagga tcttcaccta gatcctttta aattaaaaat 4981 gaagttttaa atcaatctaa agtatatatg agtaaacttg gtctgacagt taccaatgct 5041 taatcagtga ggcacctatc tcagcgatct gtctatttcg ttcatccata gttgcctgac 5101 tccccgtcgt gtagataact acgatacggg agggcttacc atctggcccc agtgctgcaa 5161 tgataccgcg agacccacgc tcaccggctc cagatttatc agcaataaac cagccagccg 5221 gaagggccga gcgcagaagt ggtcctgcaa ctttatccgc ctccatccag tctattaatt 5281 gttgccggga agctagagta agtagttcgc cagttaatag tttgcgcaac gttgttgcca 5341 ttgctgcagg catcgtggtg tcacgctcgt cgtttggtat ggcttcattc agctccggtt 5401 cccaacgatc aaggcgagtt acatgatccc ccatgttgtg caaaaaagcg gttagctcct 5461 tcggtcctcc gatcgttgtc agaagtaagt tggccgcagt gttatcactc atggttatgg 5521 cagcactgca taattctctt actgtcatgc catccgtaag atgcttttct gtgactggtg 5581 agtactcaac caagtcattc tgagaatagt gtatgcggcg accgagttgc tcttgcccgg 5641 cgtcaacacg ggataatacc gcgccacata gcagaacttt aaaagtgctc atcattggaa 5701 aacgttcttc ggggcgaaaa ctctcaagga tcttaccgct gttgagatcc agttcgatgt 5761 aacccactcg tgcacccaac tgatcttcag catcttttac tttcaccagc gtttctgggt 5821 gagcaaaaac aggaaggcaa aatgccgcaa aaaagggaat aagggcgaca cggaaatgtt 5881 gaatactcat actcttcctt tttcaatatt attgaagcat ttatcagggt tattgtctca 5941 tgagcggata catatttgaa tgtatttaga aaaataaaca aataggggtt ccgcgcacat 6001 ttccccgaaa agtgccacct gacgtctaag aaaccattat tatcatgaca ttaacctata 6061 aaaataggcg tatcacgagg ccctttcgt
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