p426-SNR52p-gRNA.csr-1.Y-SUP4t酵母CRISPR/Cas9

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货　　号：68060
产　　地：北京

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p426-SNR52p-gRNA.CAN1.Y-SUP4t

描述：

酵母CRISPR-Cas9系统质粒载体，URA3筛选标记，p426载体骨架，2u ori酵母复制起始位点，可插入guideRNA序列。

Encodes a gRNA that targets Can1.Y in yeast.

gRNA target sequence GATACGTTCTCTATGGAGGA

BACKBONE

Vector backbone
p426
Backbone size w/o insert (bp)5886
Total vector size (bp)6274
Vector type
Yeast Expression, CRISPR
Selectable markers
URA3

GROWTH IN BACTERIA

Bacterial Resistance(s)
Ampicillin
Growth Temperature
30°C
Growth Strain(s)
DH5alpha
Copy number
High Copy

GENE/INSERT

Gene/Insert name
CAN1.y gRNA
Species
S. cerevisiae (budding yeast)
Insert Size (bp)
488
Entrez Gene
CAN1(a.k.a.YEL063C)
PromoterSNR52

CLONING INFORMATION

Cloning methodUnknown
5′ sequencing primerT3 promoter
3′ sequencing primerT7 promoter

Map图谱：

p426-SNR52p-gRNA.CAN1.Y-SUP4t酵母CRISPR-Cas9系统质粒载体，URA3筛选标记，p426载体骨架，2u ori酵母复制起始位点，可插入guideRNA序列。Amp抗性。BioVector NTCC质粒载体菌种细胞基因保藏中心提供

Sequence序列：

>p426-SNR52p-gRNA.CAN1.Y-SUP4t sequence 6274 bps
gacgaaagggcctcgtgatacgcctatttttataggttaatgtcatgataataatggtttcttagtatga
tccaatatcaaaggaaatgatagcattgaaggatgagactaatccaattgaggagtggcagcatatagaa
cagctaaagggtagtgctgaaggaagcatacgataccccgcatggaatgggataatatcacaggaggtac
tagactacctttcatcctacataaatagacgcatataagtacgcatttaagcataaacacgcactatgcc
gttcttctcatgtatatatatatacaggcaacacgcagatataggtgcgacgtgaacagtgagctgtatg
tgcgcagctcgcgttgcattttcggaagcgctcgttttcggaaacgctttgaagttcctattccgaagtt
cctattctctagaaagtataggaacttcagagcgcttttgaaaaccaaaagcgctctgaagacgcacttt
caaaaaaccaaaaacgcaccggactgtaacgagctactaaaatattgcgaataccgcttccacaaacatt
gctcaaaagtatctctttgctatatatctctgtgctatatccctatataacctacccatccacctttcgc
tccttgaacttgcatctaaactcgacctctacattttttatgtttatctctagtattactctttagacaa
aaaaattgtagtaagaactattcatagagtgaatcgaaaacaatacgaaaatgtaaacatttcctatacg
tagtatatagagacaaaatagaagaaaccgttcataattttctgaccaatgaagaatcatcaacgctatc
actttctgttcacaaagtatgcgcaatccacatcggtatagaatataatcggggatgcctttatcttgaa
aaaatgcacccgcagcttcgctagtaatcagtaaacgcgggaagtggagtcaggctttttttatggaaga
gaaaatagacaccaaagtagccttcttctaaccttaacggacctacagtgcaaaaagttatcaagagact
gcattatagagcgcacaaaggagaaaaaaagtaatctaagatgctttgttagaaaaatagcgctctcggg
atgcatttttgtagaacaaaaaagaagtatagattctttgttggtaaaatagcgctctcgcgttgcattt
ctgttctgtaaaaatgcagctcagattctttgtttgaaaaattagcgctctcgcgttgcatttttgtttt
acaaaaatgaagcacagattcttcgttggtaaaatagcgctttcgcgttgcatttctgttctgtaaaaat
gcagctcagattctttgtttgaaaaattagcgctctcgcgttgcatttttgttctacaaaatgaagcaca
gatgcttcgttcaggtggcacttttcggggaaatgtgcgcggaacccctatttgtttatttttctaaata
cattcaaatatgtatccgctcatgagacaataaccctgataaatgcttcaataatattgaaaaaggaaga
gtatgagtattcaacatttccgtgtcgcccttattcccttttttgcggcattttgccttcctgtttttgc
tcacccagaaacgctggtgaaagtaaaagatgctgaagatcagttgggtgcacgagtgggttacatcgaa
ctggatctcaacagcggtaagatccttgagagttttcgccccgaagaacgttttccaatgatgagcactt
ttaaagttctgctatgtggcgcggtattatcccgtattgacgccgggcaagagcaactcggtcgccgcat
acactattctcagaatgacttggttgagtactcaccagtcacagaaaagcatcttacggatggcatgaca
gtaagagaattatgcagtgctgccataaccatgagtgataacactgcggccaacttacttctgacaacga
tcggaggaccgaaggagctaaccgcttttttgcacaacatgggggatcatgtaactcgccttgatcgttg
ggaaccggagctgaatgaagccataccaaacgacgagcgtgacaccacgatgcctgtagcaatggcaaca
acgttgcgcaaactattaactggcgaactacttactctagcttcccggcaacaattaatagactggatgg
aggcggataaagttgcaggaccacttctgcgctcggcccttccggctggctggtttattgctgataaatc
tggagccggtgagcgtgggtctcgcggtatcattgcagcactggggccagatggtaagccctcccgtatc
gtagttatctacacgacggggagtcaggcaactatggatgaacgaaatagacagatcgctgagataggtg
cctcactgattaagcattggtaactgtcagaccaagtttactcatatatactttagattgatttaaaact
tcatttttaatttaaaaggatctaggtgaagatcctttttgataatctcatgaccaaaatcccttaacgt
gagttttcgttccactgagcgtcagaccccgtagaaaagatcaaaggatcttcttgagatcctttttttc
tgcgcgtaatctgctgcttgcaaacaaaaaaaccaccgctaccagcggtggtttgtttgccggatcaaga
gctaccaactctttttccgaaggtaactggcttcagcagagcgcagataccaaatactgtccttctagtg
tagccgtagttaggccaccacttcaagaactctgtagcaccgcctacatacctcgctctgctaatcctgt
taccagtggctgctgccagtggcgataagtcgtgtcttaccgggttggactcaagacgatagttaccgga
taaggcgcagcggtcgggctgaacggggggttcgtgcacacagcccagcttggagcgaacgacctacacc
gaactgagatacctacagcgtgagctatgagaaagcgccacgcttcccgaagggagaaaggcggacaggt
atccggtaagcggcagggtcggaacaggagagcgcacgagggagcttccagggggaaacgcctggtatct
ttatagtcctgtcgggtttcgccacctctgacttgagcgtcgatttttgtgatgctcgtcaggggggcgg
agcctatggaaaaacgccagcaacgcggcctttttacggttcctggccttttgctggccttttgctcaca
tgttctttcctgcgttatcccctgattctgtggataaccgtattaccgcctttgagtgagctgataccgc
tcgccgcagccgaacgaccgagcgcagcgagtcagtgagcgaggaagcggaagagcgcccaatacgcaaa
ccgcctctccccgcgcgttggccgattcattaatgcagctggcacgacaggtttcccgactggaaagcgg
gcagtgagcgcaacgcaattaatgtgagttacctcactcattaggcaccccaggctttacactttatgct
tccggctcctatgttgtgtggaattgtgagcggataacaatttcacacaggaaacagctatgaccatgat
tacgccaagcgcgcaattaaccctcactaaagggaacaaaagctggagcttctttgaaaagataatgtat
gattatgctttcactcatatttatacagaaacttgatgttttctttcgagtatatacaaggtgattacat
gtacgtttgaagtacaactctagattttgtagtgccctcttgggctagcggtaaaggtgcgcattttttc
acaccctacaatgttctgttcaaaagattttggtcaaacgctgtagaagtgaaagttggtgcgcatgttt
cggcgttcgaaacttctccgcagtgaaagataaatgatcGATACGTTCTCTATGGAGGAGTTTTAGAGCT
AGAAATAGCAAGTTAAAATAAGGCTAGTCCGTTATCAACTTGAAAAAGTGGCACCGAGTCGGTGGTGCTT
TTTTTGTTTTTTATGTCTtcgagtcatgtaattagttatgtcacgcttacGttcacgccctccccccaca
tccgctctaaccgaaaaggaaggagttagacaacctgaagtctaggtccctatttatttttttatagtta
tgttagtattaagaacgttatttatatttcaaatttttcttttttttctgtacagacgcgtgtacgcatg
taacattatactgaaaaccttgcttgagaaggttttgggacgctcgaaggctttaatttgcggccggtac
ccaattcgccctatagtgagtcgtattacgcgcgctcactggccgtcgttttacaacgtcgtgactggga
aaaccctggcgttacccaacttaatcgccttgcagcacatccccctttcgccagctggcgtaatagcgaa
gaggcccgcaccgatcgcccttcccaacagttgcgcagcctgaatggcgaatggcgcgacgcgccctgta
gcggcgcattaagcgcggcgggtgtggtggttacgcgcagcgtgaccgctacacttgccagcgccctagc
gcccgctcctttcgctttcttcccttcctttctcgccacgttcgccggctttccccgtcaagctctaaat
cgggggctccctttagggttccgatttagtgctttacggcacctcgaccccaaaaaacttgattagggtg
atggttcacgtagtgggccatcgccctgatagacggtttttcgccctttgacgttggagtccacgttctt
taatagtggactcttgttccaaactggaacaacactcaaccctatctcggtctattcttttgatttataa
gggattttgccgatttcggcctattggttaaaaaatgagctgatttaacaaaaatttaacgcgaatttta
acaaaatattaacgtttacaatttcctgatgcggtattttctccttacgcatctgtgcggtatttcacac
cgcatagggtaataactgatataattaaattgaagctctaatttgtgagtttagtatacatgcatttact
tataatacagttttttagttttgctggccgcatcttctcaaatatgcttcccagcctgcttttctgtaac
gttcaccctctaccttagcatcccttccctttgcaaatagtcctcttccaacaataataatgtcagatcc
tgtagagaccacatcatccacggttctatactgttgacccaatgcgtctcccttgtcatctaaacccaca
ccgggtgtcataatcaaccaatcgtaaccttcatctcttccacccatgtctctttgagcaataaagccga
taacaaaatctttgtcgctcttcgcaatgtcaacagtacccttagtatattctccagtagatagggagcc
cttgcatgacaattctgctaacatcaaaaggcctctaggttcctttgttacttcttctgccgcctgcttc
aaaccgctaacaatacctgggcccaccacaccgtgtgcattcgtaatgtctgcccattctgctattctgt
atacacccgcagagtactgcaatttgactgtattaccaatgtcagcaaattttctgtcttcgaagagtaa
aaaattgtacttggcggataatgcctttagcggcttaactgtgccctccatggaaaaatcagtcaagata
tccacatgtgtttttagtaaacaaattttgggacctaatgcttcaactaactccagtaattccttggtgg
tacgaacatccaatgaagcacacaagtttgtttgcttttcgtgcatgatattaaatagcttggcagcaac
aggactaggatgagtagcagcacgttccttatatgtagctttcgacatgatttatcttcgtttcctgcag
gtttttgttctgtgcagttgggttaagaatactgggcaatttcatgtttcttcaacactacatatgcgta
tatataccaatctaagtctgtgctccttccttcgttcttccttctgttcggagattaccgaatcaaaaaa
atttcaaGgaaaccgaaatcaaaaaaaagaataaaaaaaaaatgatgaattgaattgaaaagctgtggta
tggtgcactctcagtacaatctgctctgatgccgcatagttaagccagccccgacacccgccaacacccg
ctgacgcgccctgacgggcttgtctgctcccggcatccgcttacagacaagctgtgaccgtctccgggag
ctgcatgtgtcagaggttttcaccgtcatcaccgaaacgcgcga