pYES2-NgAgo-NLS酿酒酵母NgAgo基因编辑载体-BioVector NTCC质粒载体菌种细胞基因保藏中心

pYES2-NgAgo-NLS plasmid

pYES2-NgAgo-NLS酿酒酵母NgAgo基因编辑载体，NgAgo序列根据酿酒酵母密码子偏好性进行优化设计，带有GAL1半乳糖诱导表达启动子，葡萄糖抑制表达，带有CYC1终止子，URA3尿嘧啶酵母缺陷筛选标记，Amp抗性。

pYES2 is a 5.9 kb vector designed for inducible expression of recombinant

proteins in Saccharomyces cerevisiae. Features of the vector allow easy cloning of

your gene of interest and selection of transformants by uracil prototrophy (see

pages 15–16). The vector contains the following elements:

 Yeast GAL1 promoter for high level inducible protein expression in yeast by

galactose and repression by glucose (Giniger et al., 1985; West et al., 1984)

(see page 7 for more information)

 A versatile multiple cloning site for simplified cloning

 CYC1 transcriptional terminator for efficient termination of mRNA

 URA3 gene for selection of transformants in yeast host strains with a ura3

genotype

 Ampicillin resistance gene for selection in E. coli

Map图谱：

BioVector NTCC质粒载体菌种细胞基因保藏中心