出品公司: | Invitrogen |
---|---|
载体名称: | pMIB/V5-His B |
质粒类型: | 昆虫细胞表达系统载体 |
高拷贝/低拷贝: | 高拷贝 |
启动子: | OpIE2 |
克隆方法: | 多克隆位点,限制性内切酶 |
载体大小: | 3600bp |
5' 测序引物及序列: | OpIE2-F: 5'd[CGCAACGATCTGGTAAACAC]3' |
3' 测序引物及序列: | -- |
载体标签: | HBM (Nterm), V5 (Cterm), His (Cterm) |
载体抗性: | Ampicillin |
筛选标记: | Blasticidin |
备注: | 在昆虫细胞中能够稳定表达,HBM标签可实现目的蛋白的分泌表达 |
产品目录号: | V803001 |
稳定性: | 稳表达 |
组成型: | 组成型 |
病毒/非病毒: | 非病毒 |
pMIB/V5-His B质粒载体图谱序列抗性价格报价Biovector NTCC Inc.现货
- 价 格:¥1920
- 货 号:BiovectorpMIB/V5-His B
- 产 地:北京
- BioVector NTCC典型培养物保藏中心
- 联系人:Dr.Xu, Biovector NTCC Inc.
电话:400-800-2947 工作QQ:1843439339 (微信同号)
邮件:Biovector@163.com
手机:18901268599
地址:北京
- 已注册
pMIB-V5-HisB载体基本信息
pMIB-V5-HisB载体质粒图谱和多克隆位点信息
pMIB-V5-HisB载体简介
pMIB-V5-HisB载体序列
LOCUS pMIB/V5-His B 3600 bp DNA SYN DEFINITION pMIB/V5-His B ACCESSION KEYWORDS SOURCE ORGANISM other sequences; artificial sequences; vectors. FEATURES Location/Qualifiers source 1..3600 /organism="pMIB/V5-His B" /mol_type="other DNA" misc_feature 511..530 /label="OpIE2_fwd_primer" rep_origin complement(1066..1685) /label="pBR322_origin" promoter 2059..2126 /label="EM7_promoter" gene 2127..2525 /label="blast (variant)" /gene="blast (variant)" gene 2645..3505 /label="Ampicillin" /gene="Ampicillin" CDS 2645..3505 /label="ORF frame 2" /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGY IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVE YSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRL DRWEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPL LRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIA EIGASLIKHW*" ORIGIN 1 CATGATGATA AACAATGTAT GGTGCTAATG TTGCTTCAAC AACAATTCTG TTGAACTGTG 61 TTTTCATGTT TGCCAACAAG CACCTTTATA CTCGGTGGCC TCCCCACCAC CAACTTTTTT 121 GCACTGCAAA AAAACACGCT TTTGCACGCG GGCCCATACA TAGTACAAAC TCTACGTTTC 181 GTAGACTATT TTACATAAAT AGTCTACACC GTTGTATACG CTCCAAATAC ACTACCACAC 241 ATTGAACCTT TTTGCAGTGC AAAAAAGTAC GTGTCGGCAG TCACGTAGGC CGGCCTTATC 301 GGGTCGCGTC CTGTCACGTA CGAATCACAT TATCGGACCG GACGAGTGTT GTCTTATCGT 361 GACAGGACGC CAGCTTCCTG TGTTGCTAAC CGCAGCCGGA CGCAACTCCT TATCGGAACA 421 GGACGCGCCT CCATATCAGC CGCGCGTTAT CTCATGCGCG TGACCGGACA CGAGGCGCCC 481 GTCCCGCTTA TCGCGCCTAT AAATACAGCC CGCAACGATC TGGTAAACAC AGTTGAACAG 541 CATCTGTTCG AATTTAAAGC TACCATGAAA TTCTTAGTCA ACGTTGCCCT TGTTTTTATG 601 GTCGTATACA TTTCTTACAT CTATGCCGGC ATGCTAAGCT TGGTACCGAG CTCGGATCCA 661 CTAGTCCAGT GTGGTGGAAT TCTGCAGATA TCCAGCACAG TGGCGGCCGC TCGAGTCTAG 721 AGGGCCCGCG GTTCGAAGGT AAGCCTATCC CTAACCCTCT CCTCGGTCTC GATTCTACGC 781 GTACCGGTCA TCATCACCAT CACCATTGAG TTTATCTGAC TAAATCTTAG TTTGTATTGT 841 CATGTTTTAA TACAATATGT TATGTTTAAA TATGTTTTTA ATAAATTTTA TAAAATAATT 901 TCAACTTTTA TTGTAACAAC ATTGTCCATT TACACACTCC TTTCAAGCGC GTGGGATCGA 961 TGCTCACTCA AAGGCGGTAA TACGGTTATC CACAGAATCA GGGGATAACG CAGGAAAGAA 1021 CATGTGAGCA AAAGGCCAGC AAAAGGCCAG GAACCGTAAA AAGGCCGCGT TGCTGGCGTT 1081 TTTCCATAGG CTCCGCCCCC CTGACGAGCA TCACAAAAAT CGACGCTCAA GTCAGAGGTG 1141 GCGAAACCCG ACAGGACTAT AAAGATACCA GGCGTTTCCC CCTGGAAGCT CCCTCGTGCG 1201 CTCTCCTGTT CCGACCCTGC CGCTTACCGG ATACCTGTCC GCCTTTCTCC CTTCGGGAAG 1261 CGTGGCGCTT TCTCATAGCT CACGCTGTAG GTATCTCAGT TCGGTGTAGG TCGTTCGCTC 1321 CAAGCTGGGC TGTGTGCACG AACCCCCCGT TCAGCCCGAC CGCTGCGCCT TATCCGGTAA 1381 CTATCGTCTT GAGTCCAACC CGGTAAGACA CGACTTATCG CCACTGGCAG CAGCCACTGG 1441 TAACAGGATT AGCAGAGCGA GGTATGTAGG CGGTGCTACA GAGTTCTTGA AGTGGTGGCC 1501 TAACTACGGC TACACTAGAA GAACAGTATT TGGTATCTGC GCTCTGCTGA AGCCAGTTAC 1561 CTTCGGAAAA AGAGTTGGTA GCTCTTGATC CGGCAAACAA ACCACCGCTG GTAGCGGTGG 1621 TTTTTTTGTT TGCAAGCAGC AGATTACGCG CAGAAAAAAA GGATCTCAAG AAGATCCTTT 1681 GATCTTTTCT ACGGGGTCTG ACGCTCAGTG GAACGAAAAC TCACGTTAAG GGATTTTGGT 1741 CATGCGAAAC ACGCACGGCG CGCGCACGCA GCTTAGCACA AACGCGTCGT TGCACGCGCC 1801 CACCGCTAAC CGCAGGCCAA TCGGTCGGCC GGCCTCATAT CCGCTCACCA GCCGCGTCCT 1861 ATCGGGCGCG GCTTCCGCGC CCATTTTGAA TAAATAAACG ATAACGCCGT TGGTGGCGTG 1921 AGGCATGTAA AAGGTTACAT CATTATCTTG TTCGCCATCC GGTTGGTATA AATAGACGTT 1981 CATGTTGGTT TTTGTTTCAG TTGCAAGTTG GCTGCGGCGC GCGCAGCACC TTTGCCGGGA 2041 TCTGCCGGGC TGCAGCACGT GTTGACAATT AATCATCGGC ATAGTATATC GGCATAGTAT 2101 AATACGACAA GGTGAGGAAC TAAACCATGG CCAAGCCTTT GTCTCAAGAA GAATCCACCC 2161 TCATTGAAAG AGCAACGGCT ACAATCAACA GCATCCCCAT CTCTGAAGAC TACAGCGTCG 2221 CCAGCGCAGC TCTCTCTAGC GACGGCCGCA TCTTCACTGG TGTCAATGTA TATCATTTTA 2281 CTGGGGGACC TTGCGCAGAA CTCGTGGTGC TGGGCACTGC TGCTGCTGCG GCAGCTGGCA 2341 ACCTGACTTG TATCGTCGCG ATCGGAAATG AGAACAGGGG CATCTTGAGC CCCTGCGGAC 2401 GGTGCCGACA GGTTCTTCTC GATCTGCATC CTGGGATCAA AGCCATAGTG AAGGACAGTG 2461 ATGGACAGCC GACGGCAGTT GGGATTCGTG AATTGCTGCC CTCTGGTTAT GTGTGGGAGG 2521 GCTAAGCACT TCGTGGCCGA GGAGCAGGAC TGACACGTCC CGGGAGATCT GCATGTCTAC 2581 TAAACTCACA AATTAGAGCT TCAATTTAAT TATATCAGTT ATTACCCATT GAAAAAGGAA 2641 GAGTATGAGT ATTCAACATT TCCGTGTCGC CCTTATTCCC TTTTTTGCGG CATTTTGCCT 2701 TCCTGTTTTT GCTCACCCAG AAACGCTGGT GAAAGTAAAA GATGCTGAAG ATCAGTTGGG 2761 TGCACGAGTG GGTTACATCG AACTGGATCT CAACAGCGGT AAGATCCTTG AGAGTTTTCG 2821 CCCCGAAGAA CGTTTTCCAA TGATGAGCAC TTTTAAAGTT CTGCTATGTG GCGCGGTATT 2881 ATCCCGTATT GACGCCGGGC AAGAGCAACT CGGTCGCCGC ATACACTATT CTCAGAATGA 2941 CTTGGTTGAG TACTCACCAG TCACAGAAAA GCATCTTACG GATGGCATGA CAGTAAGAGA 3001 ATTATGCAGT GCTGCCATAA CCATGAGTGA TAACACTGCG GCCAACTTAC TTCTGACAAC 3061 GATCGGAGGA CCGAAGGAGC TAACCGCTTT TTTGCACAAC ATGGGGGATC ATGTAACTCG 3121 CCTTGATCGT TGGGAACCGG AGCTGAATGA AGCCATACCA AACGACGAGC GTGACACCAC 3181 GATGCCTGTA GCAATGGCAA CAACGTTGCG CAAACTATTA ACTGGCGAAC TACTTACTCT 3241 AGCTTCCCGG CAACAATTAA TAGACTGGAT GGAGGCGGAT AAAGTTGCAG GACCACTTCT 3301 GCGCTCGGCC CTTCCGGCTG GCTGGTTTAT TGCTGATAAA TCTGGAGCCG GTGAGCGTGG 3361 GTCTCGCGGT ATCATTGCAG CACTGGGGCC AGATGGTAAG CCCTCCCGTA TCGTAGTTAT 3421 CTACACGACG GGGAGTCAGG CAACTATGGA TGAACGAAAT AGACAGATCG CTGAGATAGG 3481 TGCCTCACTG ATTAAGCATT GGTAACTGTC AGACCAAGTT TACTCATATA TACTTTAGAT 3541 TGATTTAAAA CTTCATTTTT AATTTAAAAG GATCTAGGTG AAGATCCTTT TTGATAATCT
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