pNOY3079 出芽酵母载体质粒BioVector NTCC质粒载体菌种细胞基因保藏中心Budding yeast vector

pNOY3079  【载体类型Vector Type】：YCp  【插入基因Inserted Gene】： RPA190  【大肠杆菌筛选标记E.coli selection marker】： Amp  【酵母筛选标记S.pombe selection marker】： TRP1  【其他Comments】: BclI/EcoRI (EcoRI sute blunted with Klenow) RPA190 fragment from pNOY3073 cloned into BamHI/SalI (SalI site blunted with Klenow) cut pTC3 (pNOY14). Only orientation obtained was opposite from the expected one. This plasmid will not complement rpa190Δ::URA3 K.O. Part of promoter sequence is missing, Mike Wittekind