pNOY3300, pNOY3298ΔE/T 出芽酵母载体质粒BioVector NTCC质粒载体菌种细胞基因保藏中心Budding yeast vector

pNOY3300, pNOY3298ΔE/T  【载体类型Vector Type】： 【插入基因Inserted Gene】： CEN5RE-At NMD3-rDNA-HIS3- CEN5LE  【大肠杆菌筛选标记E.coli selection marker】： Amp  【酵母筛选标记S.pombe selection marker】： HIS3  【其他Comments】: pNOY3298 was digested with BamHI-NotI to remove the 583 bp fragment containing Enhancer-terminator upstream of PolI promoter. Plasmid was blunt ended by T4 DNA polymerase and self-ligated. 9.1 kb repeat in opposite direction from pNOY3291, Loan Vu