出品公司: | Cambia |
---|---|
载体名称: | pCambia1391Z, pCambia 1391Z |
质粒类型: | 植物表达载体 |
高拷贝/低拷贝: | 低拷贝 |
启动子: | CAMV 35S |
克隆方法: | 多克隆位点,限制性内切酶 |
载体大小: | 11227 bp |
5' 测序引物及序列: | M13-F: TGTAAAACGACGGCCAGT |
3' 测序引物及序列: | M13-R: CAGGAAACAGCTATGAC |
载体标签: | GusA |
载体抗性: | 卡那和潮霉素 |
筛选标记: | HPTII |
备注: | -- |
产品目录号: | 1391Z |
稳定性: | 稳定表达 |
组成型: | 非组成型 |
病毒/非病毒: | 非病毒 |
pCambia1391Z质粒载体图谱序列抗性价格报价Biovector NTCC Inc.现货
- 价 格:¥1920
- 货 号:BiovectorpCambia1391Z
- 产 地:北京
- BioVector NTCC典型培养物保藏中心
- 联系人:Dr.Xu, Biovector NTCC Inc.
电话:400-800-2947 工作QQ:1843439339 (微信同号)
邮件:Biovector@163.com
手机:18901268599
地址:北京
- 已注册
pCambia 1391Z载体基本信息
pCambia 1391Z载体质粒图谱和多克隆位点信息
pCambia 1391Z载体简介
pCambia 1391Z载体序列
LOCUS pCAMBIA1391Z 11227 bp ds-DNA circular SYN 01-十二月-2013 DEFINITION Agrobacterium binary vector for plant transformation, with hygromycin- and kanamycin-resistance and LacZ-GUS genes plus the pUC9 MCS. ACCESSION AF234312 VERSION . KEYWORDS pCAMBIA1391Z SOURCE synthetic DNA construct ORGANISM synthetic DNA construct REFERENCE 1 (bases 1 to 11227) AUTHORS Cambia TITLE Direct Submission JOURNAL Exported 2014年6月2日 from SnapGene Viewer 1.5.3 http://www.snapgene.com COMMENT The GenBank record was corrected by inserting a G at position 4743. FEATURES Location/Qualifiers source 1..11227 /organism="synthetic DNA construct" /lab_host="Plant Cells" /mol_type="other DNA" CDS join(2..16,207..2024) /codon_start=1 /product="beta-glucuronidase" /label="GUS" /label="This version of the gusA gene has a 5' extension with a catalase intron to ensure expression in plants but not bacteria." /protein_id=" " intron 17..206 /label="cat1 intron" /label="castor bean catalase intron, modified" CDS 2031..2048 /codon_start=1 /product="6xHis affinity tag" /label="6xHis" /translation="HHHHHH" terminator 2083..2335 /label="NOS terminator" /label="nopaline synthase terminator and poly(A) signal" misc_feature 2357..2381 /label="RB T-DNA repeat" /label="right border repeat from nopaline C58 T-DNA" CDS 3681..4310 /codon_start=1 /product="stability protein from Pseudomonas plasmid pVS1" /label="pVS1 StaA" CDS 4739..5812 /codon_start=1 /product="replication protein from Pseudomonas plasmid pVS1" /label="pVS1 RepA" /label=" " /protein_id=" " misc_feature 6416..6556 /label="bom" /label="basis of mobility region from pBR322" rep_origin complement(6742..7330) /direction=LEFT /label="ori" /label="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication" CDS complement(7417..8211) /codon_start=1 /gene="aphA-3" /product="aminoglycoside phosphotransferase" /label="KanR" /label="confers resistance to kanamycin" /protein_id=" " misc_feature 8636..8660 /label="LB T-DNA repeat" /label="left border repeat from nopaline C58 T-DNA" polyA_signal 8738..8912 /label="CaMV poly(A) signal" /label="cauliflower mosaic virus polyadenylation signal" CDS complement(8952..9977) /codon_start=1 /product="hygromycin B phosphotransferase" /label="HygR" /label="confers resistance to hygromycin" promoter complement(10045..10722) /label="CaMV 35S promoter (enhanced)" /label="cauliflower mosaic virus 35S promoter with a duplicated enhancer region" promoter 10936..10966 /label="lac promoter" /label="promoter for the E. coli lac operon" protein_bind 10974..10990 /bound_moiety="lac repressor encoded by lacI" /label="lac operator" /label="The lac repressor binds to the lac operator to inhibit transcription in E. coli. This inhibition can be relieved by adding lactose or isopropyl-beta-D-thiogalactopyranoside (IPTG)." primer_bind 10998..11014 /label="M13 rev" /label="common sequencing primer, one of multiple similar variants" CDS 11010..11207 /codon_start=1 /gene="lacZ (fragment)" /product="LacZ-alpha fragment of?beta-galactosidase" /label="lacZ-alpha" /protein_id=" " /translation="MTMITPSLAAGRRIPGNSLAVVLQRRDWENPGVTQLNRLAAHPPF ASWRNSEEARTDRPSQQLRSL" misc_feature 11027..11062 /label="MCS" /label="multiple cloning site from pUC9" primer_bind complement(11063..11079) /label="M13 fwd" /label="common sequencing primer, one of multiple similar variants" ORIGIN 1 catggtagat ctgagggtaa atttctagtt tttctccttc attttcttgg ttaggaccct 61 tttctctttt tatttttttg agctttgatc tttctttaaa ctgatctatt ttttaattga 121 ttggttatgg tgtaaatatt acatagcttt aactgataat ctgattactt tatttcgtgt 181 gtctatgatg atgatgatag ttacagaacc gacgactcgt ccgtcctgta gaaaccccaa 241 cccgtgaaat caaaaaactc gacggcctgt gggcattcag tctggatcgc gaaaactgtg 301 gaattgatca gcgttggtgg gaaagcgcgt tacaagaaag ccgggcaatt gctgtgccag 361 gcagttttaa cgatcagttc gccgatgcag atattcgtaa ttatgcgggc aacgtctggt 421 atcagcgcga agtctttata ccgaaaggtt gggcaggcca gcgtatcgtg ctgcgtttcg 481 atgcggtcac tcattacggc aaagtgtggg tcaataatca ggaagtgatg gagcatcagg 541 gcggctatac gccatttgaa gccgatgtca cgccgtatgt tattgccggg aaaagtgtac 601 gtatcaccgt ttgtgtgaac aacgaactga actggcagac tatcccgccg ggaatggtga 661 ttaccgacga aaacggcaag aaaaagcagt cttacttcca tgatttcttt aactatgccg 721 gaatccatcg cagcgtaatg ctctacacca cgccgaacac ctgggtggac gatatcaccg 781 tggtgacgca tgtcgcgcaa gactgtaacc acgcgtctgt tgactggcag gtggtggcca 841 atggtgatgt cagcgttgaa ctgcgtgatg cggatcaaca ggtggttgca actggacaag 901 gcactagcgg gactttgcaa gtggtgaatc cgcacctctg gcaaccgggt gaaggttatc 961 tctatgaact cgaagtcaca gccaaaagcc agacagagtc tgatatctac ccgcttcgcg 1021 tcggcatccg gtcagtggca gtgaagggcc aacagttcct gattaaccac aaaccgttct 1081 actttactgg ctttggtcgt catgaagatg cggacttacg tggcaaagga ttcgataacg 1141 tgctgatggt gcacgaccac gcattaatgg actggattgg ggccaactcc taccgtacct 1201 cgcattaccc ttacgctgaa gagatgctcg actgggcaga tgaacatggc atcgtggtga 1261 ttgatgaaac tgctgctgtc ggctttcagc tgtctttagg cattggtttc gaagcgggca 1321 acaagccgaa agaactgtac agcgaagagg cagtcaacgg ggaaactcag caagcgcact 1381 tacaggcgat taaagagctg atagcgcgtg acaaaaacca cccaagcgtg gtgatgtgga 1441 gtattgccaa cgaaccggat acccgtccgc aaggtgcacg ggaatatttc gcgccactgg 1501 cggaagcaac gcgtaaactc gacccgacgc gtccgatcac ctgcgtcaat gtaatgttct 1561 gcgacgctca caccgatacc atcagcgatc tctttgatgt gctgtgcctg aaccgttatt 1621 acggatggta tgtccaaagc ggcgatttgg aaacggcaga gaaggtactg gaaaaagaac 1681 ttctggcctg gcaggagaaa ctgcatcagc cgattatcat caccgaatac ggcgtggata 1741 cgttagccgg gctgcactca atgtacaccg acatgtggag tgaagagtat cagtgtgcat 1801 ggctggatat gtatcaccgc gtctttgatc gcgtcagcgc cgtcgtcggt gaacaggtat 1861 ggaatttcgc cgattttgcg acctcgcaag gcatattgcg cgttggcggt aacaagaaag 1921 ggatcttcac tcgcgaccgc aaaccgaagt cggcggcttt tctgctgcaa aaacgctgga 1981 ctggcatgaa cttcggtgaa aaaccgcagc agggaggcaa acaagctagc caccaccacc 2041 accaccacgt gtgaattaca ggtgaccagc tcgaatttcc ccgatcgttc aaacatttgg 2101 caataaagtt tcttaagatt gaatcctgtt gccggtcttg cgatgattat catataattt 2161 ctgttgaatt acgttaagca tgtaataatt aacatgtaat gcatgacgtt atttatgaga 2221 tgggttttta tgattagagt cccgcaatta tacatttaat acgcgataga aaacaaaata 2281 tagcgcgcaa actaggataa attatcgcgc gcggtgtcat ctatgttact agatcgggaa 2341 ttaaactatc agtgtttgac aggatatatt ggcgggtaaa cctaagagaa aagagcgttt 2401 attagaataa cggatattta aaagggcgtg aaaaggttta tccgttcgtc catttgtatg 2461 tgcatgccaa ccacagggtt cccctcggga tcaaagtact ttgatccaac ccctccgctg 2521 ctatagtgca gtcggcttct gacgttcagt gcagccgtct tctgaaaacg acatgtcgca 2581 caagtcctaa gttacgcgac aggctgccgc cctgcccttt tcctggcgtt ttcttgtcgc 2641 gtgttttagt cgcataaagt agaatacttg cgactagaac cggagacatt acgccatgaa 2701 caagagcgcc gccgctggcc tgctgggcta tgcccgcgtc agcaccgacg accaggactt 2761 gaccaaccaa cgggccgaac tgcacgcggc cggctgcacc aagctgtttt ccgagaagat 2821 caccggcacc aggcgcgacc gcccggagct ggccaggatg cttgaccacc tacgccctgg 2881 cgacgttgtg acagtgacca ggctagaccg cctggcccgc agcacccgcg acctactgga 2941 cattgccgag cgcatccagg aggccggcgc gggcctgcgt agcctggcag agccgtgggc 3001 cgacaccacc acgccggccg gccgcatggt gttgaccgtg ttcgccggca ttgccgagtt 3061 cgagcgttcc ctaatcatcg accgcacccg gagcgggcgc gaggccgcca aggcccgagg 3121 cgtgaagttt ggcccccgcc ctaccctcac cccggcacag atcgcgcacg cccgcgagct 3181 gatcgaccag gaaggccgca ccgtgaaaga ggcggctgca ctgcttggcg tgcatcgctc 3241 gaccctgtac cgcgcacttg agcgcagcga ggaagtgacg cccaccgagg ccaggcggcg 3301 cggtgccttc cgtgaggacg cattgaccga ggccgacgcc ctggcggccg ccgagaatga 3361 acgccaagag gaacaagcat gaaaccgcac caggacggcc aggacgaacc gtttttcatt 3421 accgaagaga tcgaggcgga gatgatcgcg gccgggtacg tgttcgagcc gcccgcgcac 3481 gtctcaaccg tgcggctgca tgaaatcctg gccggtttgt ctgatgccaa gctggcggcc 3541 tggccggcca gcttggccgc tgaagaaacc gagcgccgcc gtctaaaaag gtgatgtgta 3601 tttgagtaaa acagcttgcg tcatgcggtc gctgcgtata tgatgcgatg agtaaataaa 3661 caaatacgca aggggaacgc atgaaggtta tcgctgtact taaccagaaa ggcgggtcag 3721 gcaagacgac catcgcaacc catctagccc gcgccctgca actcgccggg gccgatgttc 3781 tgttagtcga ttccgatccc cagggcagtg cccgcgattg ggcggccgtg cgggaagatc 3841 aaccgctaac cgttgtcggc atcgaccgcc cgacgattga ccgcgacgtg aaggccatcg 3901 gccggcgcga cttcgtagtg atcgacggag cgccccaggc ggcggacttg gctgtgtccg 3961 cgatcaaggc agccgacttc gtgctgattc cggtgcagcc aagcccttac gacatatggg 4021 ccaccgccga cctggtggag ctggttaagc agcgcattga ggtcacggat ggaaggctac 4081 aagcggcctt tgtcgtgtcg cgggcgatca aaggcacgcg catcggcggt gaggttgccg 4141 aggcgctggc cgggtacgag ctgcccattc ttgagtcccg tatcacgcag cgcgtgagct 4201 acccaggcac tgccgccgcc ggcacaaccg ttcttgaatc agaacccgag ggcgacgctg 4261 cccgcgaggt ccaggcgctg gccgctgaaa ttaaatcaaa actcatttga gttaatgagg 4321 taaagagaaa atgagcaaaa gcacaaacac gctaagtgcc ggccgtccga gcgcacgcag 4381 cagcaaggct gcaacgttgg ccagcctggc agacacgcca gccatgaagc gggtcaactt 4441 tcagttgccg gcggaggatc acaccaagct gaagatgtac gcggtacgcc aaggcaagac 4501 cattaccgag ctgctatctg aatacatcgc gcagctacca gagtaaatga gcaaatgaat 4561 aaatgagtag atgaatttta gcggctaaag gaggcggcat ggaaaatcaa gaacaaccag 4621 gcaccgacgc cgtggaatgc cccatgtgtg gaggaacggg cggttggcca ggcgtaagcg 4681 gctgggttgt ctgccggccc tgcaatggca ctggaacccc caagcccgag gaatcggcgt 4741 gagcggtcgc aaaccatccg gcccggtaca aatcggcgcg gcgctgggtg atgacctggt 4801 ggagaagttg aaggccgcgc aggccgccca gcggcaacgc atcgaggcag aagcacgccc 4861 cggtgaatcg tggcaagcgg ccgctgatcg aatccgcaaa gaatcccggc aaccgccggc 4921 agccggtgcg ccgtcgatta ggaagccgcc caagggcgac gagcaaccag attttttcgt 4981 tccgatgctc tatgacgtgg gcacccgcga tagtcgcagc atcatggacg tggccgtttt 5041 ccgtctgtcg aagcgtgacc gacgagctgg cgaggtgatc cgctacgagc ttccagacgg 5101 gcacgtagag gtttccgcag ggccggccgg catggccagt gtgtgggatt acgacctggt 5161 actgatggcg gtttcccatc taaccgaatc catgaaccga taccgggaag ggaagggaga 5221 caagcccggc cgcgtgttcc gtccacacgt tgcggacgta ctcaagttct gccggcgagc 5281 cgatggcgga aagcagaaag acgacctggt agaaacctgc attcggttaa acaccacgca 5341 cgttgccatg cagcgtacga agaaggccaa gaacggccgc ctggtgacgg tatccgaggg 5401 tgaagccttg attagccgct acaagatcgt aaagagcgaa accgggcggc cggagtacat 5461 cgagatcgag ctagctgatt ggatgtaccg cgagatcaca gaaggcaaga acccggacgt 5521 gctgacggtt caccccgatt actttttgat cgatcccggc atcggccgtt ttctctaccg 5581 cctggcacgc cgcgccgcag gcaaggcaga agccagatgg ttgttcaaga cgatctacga 5641 acgcagtggc agcgccggag agttcaagaa gttctgtttc accgtgcgca agctgatcgg 5701 gtcaaatgac ctgccggagt acgatttgaa ggaggaggcg gggcaggctg gcccgatcct 5761 agtcatgcgc taccgcaacc tgatcgaggg cgaagcatcc gccggttcct aatgtacgga 5821 gcagatgcta gggcaaattg ccctagcagg ggaaaaaggt cgaaaaggtc tctttcctgt 5881 ggatagcacg tacattggga acccaaagcc gtacattggg aaccggaacc cgtacattgg 5941 gaacccaaag ccgtacattg ggaaccggtc acacatgtaa gtgactgata taaaagagaa 6001 aaaaggcgat ttttccgcct aaaactcttt aaaacttatt aaaactctta aaacccgcct 6061 ggcctgtgca taactgtctg gccagcgcac agccgaagag ctgcaaaaag cgcctaccct 6121 tcggtcgctg cgctccctac gccccgccgc ttcgcgtcgg cctatcgcgg ccgctggccg 6181 ctcaaaaatg gctggcctac ggccaggcaa tctaccaggg cgcggacaag ccgcgccgtc 6241 gccactcgac cgccggcgcc cacatcaagg caccctgcct cgcgcgtttc ggtgatgacg 6301 gtgaaaacct ctgacacatg cagctcccgg agacggtcac agcttgtctg taagcggatg 6361 ccgggagcag acaagcccgt cagggcgcgt cagcgggtgt tggcgggtgt cggggcgcag 6421 ccatgaccca gtcacgtagc gatagcggag tgtatactgg cttaactatg cggcatcaga 6481 gcagattgta ctgagagtgc accatatgcg gtgtgaaata ccgcacagat gcgtaaggag 6541 aaaataccgc atcaggcgct cttccgcttc ctcgctcact gactcgctgc gctcggtcgt 6601 tcggctgcgg cgagcggtat cagctcactc aaaggcggta atacggttat ccacagaatc 6661 aggggataac gcaggaaaga acatgtgagc aaaaggccag caaaaggcca ggaaccgtaa 6721 aaaggccgcg ttgctggcgt ttttccatag gctccgcccc cctgacgagc atcacaaaaa 6781 tcgacgctca agtcagaggt ggcgaaaccc gacaggacta taaagatacc aggcgtttcc 6841 ccctggaagc tccctcgtgc gctctcctgt tccgaccctg ccgcttaccg gatacctgtc 6901 cgcctttctc ccttcgggaa gcgtggcgct ttctcatagc tcacgctgta ggtatctcag 6961 ttcggtgtag gtcgttcgct ccaagctggg ctgtgtgcac gaaccccccg ttcagcccga 7021 ccgctgcgcc ttatccggta actatcgtct tgagtccaac ccggtaagac acgacttatc 7081 gccactggca gcagccactg gtaacaggat tagcagagcg aggtatgtag gcggtgctac 7141 agagttcttg aagtggtggc ctaactacgg ctacactaga aggacagtat ttggtatctg 7201 cgctctgctg aagccagtta ccttcggaaa aagagttggt agctcttgat ccggcaaaca 7261 aaccaccgct ggtagcggtg gtttttttgt ttgcaagcag cagattacgc gcagaaaaaa 7321 aggatctcaa gaagatcctt tgatcttttc tacggggtct gacgctcagt ggaacgaaaa 7381 ctcacgttaa gggattttgg tcatgcattc taggtactaa aacaattcat ccagtaaaat 7441 ataatatttt attttctccc aatcaggctt gatccccagt aagtcaaaaa atagctcgac 7501 atactgttct tccccgatat cctccctgat cgaccggacg cagaaggcaa tgtcatacca 7561 cttgtccgcc ctgccgcttc tcccaagatc aataaagcca cttactttgc catctttcac 7621 aaagatgttg ctgtctccca ggtcgccgtg ggaaaagaca agttcctctt cgggcttttc 7681 cgtctttaaa aaatcataca gctcgcgcgg atctttaaat ggagtgtctt cttcccagtt 7741 ttcgcaatcc acatcggcca gatcgttatt cagtaagtaa tccaattcgg ctaagcggct 7801 gtctaagcta ttcgtatagg gacaatccga tatgtcgatg gagtgaaaga gcctgatgca 7861 ctccgcatac agctcgataa tcttttcagg gctttgttca tcttcatact cttccgagca 7921 aaggacgcca tcggcctcac tcatgagcag attgctccag ccatcatgcc gttcaaagtg 7981 caggaccttt ggaacaggca gctttccttc cagccatagc atcatgtcct tttcccgttc 8041 cacatcatag gtggtccctt tataccggct gtccgtcatt tttaaatata ggttttcatt 8101 ttctcccacc agcttatata ccttagcagg agacattcct tccgtatctt ttacgcagcg 8161 gtatttttcg atcagttttt tcaattccgg tgatattctc attttagcca tttattattt 8221 ccttcctctt ttctacagta tttaaagata ccccaagaag ctaattataa caagacgaac 8281 tccaattcac tgttccttgc attctaaaac cttaaatacc agaaaacagc tttttcaaag 8341 ttgttttcaa agttggcgta taacatagta tcgacggagc cgattttgaa accgcggtga 8401 tcacaggcag caacgctctg tcatcgttac aatcaacatg ctaccctccg cgagatcatc 8461 cgtgtttcaa acccggcagc ttagttgccg ttcttccgaa tagcatcggt aacatgagca 8521 aagtctgccg ccttacaacg gctctcccgc tgacgccgtc ccggactgat gggctgcctg 8581 tatcgagtgg tgattttgtg ccgagctgcc ggtcggggag ctgttggctg gctggtggca 8641 ggatatattg tggtgtaaac aaattgacgc ttagacaact taataacaca ttgcggacgt 8701 ttttaatgta ctgaattaac gccgaattaa ttcgggggat ctggatttta gtactggatt 8761 ttggttttag gaattagaaa ttttattgat agaagtattt tacaaataca aatacatact 8821 aagggtttct tatatgctca acacatgagc gaaaccctat aggaacccta attcccttat 8881 ctgggaacta ctcacacatt attatggaga aactcgagct tgtcgatcga cagatccggt 8941 cggcatctac tctatttctt tgccctcgga cgagtgctgg ggcgtcggtt tccactatcg 9001 gcgagtactt ctacacagcc atcggtccag acggccgcgc ttctgcgggc gatttgtgta 9061 cgcccgacag tcccggctcc ggatcggacg attgcgtcgc atcgaccctg cgcccaagct 9121 gcatcatcga aattgccgtc aaccaagctc tgatagagtt ggtcaagacc aatgcggagc 9181 atatacgccc ggagtcgtgg cgatcctgca agctccggat gcctccgctc gaagtagcgc 9241 gtctgctgct ccatacaagc caaccacggc ctccagaaga agatgttggc gacctcgtat 9301 tgggaatccc cgaacatcgc ctcgctccag tcaatgaccg ctgttatgcg gccattgtcc 9361 gtcaggacat tgttggagcc gaaatccgcg tgcacgaggt gccggacttc ggggcagtcc 9421 tcggcccaaa gcatcagctc atcgagagcc tgcgcgacgg acgcactgac ggtgtcgtcc 9481 atcacagttt gccagtgata cacatgggga tcagcaatcg cgcatatgaa atcacgccat 9541 gtagtgtatt gaccgattcc ttgcggtccg aatgggccga acccgctcgt ctggctaaga 9601 tcggccgcag cgatcgcatc catagcctcc gcgaccggtt gtagaacagc gggcagttcg 9661 gtttcaggca ggtcttgcaa cgtgacaccc tgtgcacggc gggagatgca ataggtcagg 9721 ctctcgctaa actccccaat gtcaagcact tccggaatcg ggagcgcggc cgatgcaaag 9781 tgccgataaa cataacgatc tttgtagaaa ccatcggcgc agctatttac ccgcaggaca 9841 tatccacgcc ctcctacatc gaagctgaaa gcacgagatt cttcgccctc cgagagctgc 9901 atcaggtcgg agacgctgtc gaacttttcg atcagaaact tctcgacaga cgtcgcggtg 9961 agttcaggct ttttcatatc tcattgcccc ccgggatctg cgaaagctcg agagagatag 10021 atttgtagag agagactggt gatttcagcg tgtcctctcc aaatgaaatg aacttcctta 10081 tatagaggaa ggtcttgcga aggatagtgg gattgtgcgt catcccttac gtcagtggag 10141 atatcacatc aatccacttg ctttgaagac gtggttggaa cgtcttcttt ttccacgatg 10201 ctcctcgtgg gtgggggtcc atctttggga ccactgtcgg cagaggcatc ttgaacgata 10261 gcctttcctt tatcgcaatg atggcatttg taggtgccac cttccttttc tactgtcctt 10321 ttgatgaagt gacagatagc tgggcaatgg aatccgagga ggtttcccga tattaccctt 10381 tgttgaaaag tctcaatagc cctttggtct tctgagactg tatctttgat attcttggag 10441 tagacgagag tgtcgtgctc caccatgtta tcacatcaat ccacttgctt tgaagacgtg 10501 gttggaacgt cttctttttc cacgatgctc ctcgtgggtg ggggtccatc tttgggacca 10561 ctgtcggcag aggcatcttg aacgatagcc tttcctttat cgcaatgatg gcatttgtag 10621 gtgccacctt ccttttctac tgtccttttg atgaagtgac agatagctgg gcaatggaat 10681 ccgaggaggt ttcccgatat taccctttgt tgaaaagtct caatagccct ttggtcttct 10741 gagactgtat ctttgatatt cttggagtag acgagagtgt cgtgctccac catgttggcc 10801 aatacgcaaa ccgcctctcc ccgcgcgttg gccgattcat taatgcagct ggcacgacag 10861 gtttcccgac tggaaagcgg gcagtgagcg caacgcaatt aatgtgagtt agctcactca 10921 ttaggcaccc caggctttac actttatgct tccggctcgt atgttgtgtg gaattgtgag 10981 cggataacaa tttcacacag gaaacagcta tgaccatgat tacgccaagc ttggctgcag 11041 gtcgacggat ccccgggaat tcactggccg tcgttttaca acgtcgtgac tgggaaaacc 11101 ctggcgttac ccaacttaat cgccttgcag cacatccccc tttcgccagc tggcgtaata 11161 gcgaagaggc ccgcaccgat cgcccttccc aacagttgcg cagcctgaag cctagggagg 11221 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