AG10773 APPARENTLY HEALTHY INDIVIDUAL Vein, iliac Cell Line NTCC®细胞株-BioVector NTCC细胞库

Name细胞名称：NTCC® AG10773 APPARENTLY HEALTHY INDIVIDUAL Vein, iliac Cell Line
明显健康的个人细胞株
Description描述：Line HIVE-86. The iliac vein was removed from a braindead, heartbeating cadaver, organ donor on 6/03/87. The donor died of a gun shot wound to the head and had no known diseases. The culture was initiated on 6/03/87 using cells released from the lumen of the vessel by collagenase digestion. Cell morphology is polygonal. The culture was grown on gelatin-coated flasks and the medium was supplemented with 0.02 mg/ml ECGS and 0.05 mg/ml heparin. Cells were frozen in growth medium containing 7.5% polyvinylpyrrolidinone and 5% DMSO. For lifespan determination this cell line was cultured in Ham's F12 medium and MCDB104 medium in a 1:1 mixture with 10% inactivated FBS and supplements (0.04-0.06mg/ml crude ECGF and 0.05mg/ml heparin). This culture tested positive for the endothelial cell-specific von Willebrand factor and negative for alpha-smooth muscle actin (immunofluorescence assays). microgravity experiment in space (Mission STS-89/Mir).
Category分类：Endothelial
ID编号:NTCC®-AG10773
Size/Quantity数量: 1 Flask/2 Vials
Biosafety Level生物安全级别:1
Shipping Info运输方式: RT/Dry Ice
Storage储存方式: RT/Liquid Nitrogen
Race: White
Age年龄:16 YR
Gender性别:Male
Cell Type细胞类型: APPARENTLY HEALTHY INDIVIDUAL
Affected State:No
Product:Endothelial
Gene:
Mutations突变:
Ethnicity:
Family:
Relationship:proband
Karyotype:
Tissue Type组织来源:Vein
Complete Growth Medium完全培养基: BioVector® Medium 199 in Earl BSS with 2mM L-glutamine or equivalent Complete Medium
Subculturing传代方法:
Cryopreservation冻存方法:Freeze medium: Complete growth medium supplemented with 5% (v/v) DMSO
Storage temperature: liquid nitrogen vapor phase
Culture Conditions培养条件:Atmosphere: air, 95%; carbon dioxide (CO2), 5%
Temperature: 37.0°C
STR Profile鉴定数据:
References参考文献:

Supplier供应商:BioVector质粒载体菌株细胞蛋白抗体基因保藏中心
NTCC典型培养物保藏中心
Tel电话：400-800-2947
Email：Biovector@163.com
http://www.biovector.net