AG10768 APPARENTLY HEALTHY INDIVIDUAL Vein, iliac Cell Line NTCC®细胞株-BioVector NTCC细胞库

Name细胞名称：NTCC® AG10768 APPARENTLY HEALTHY INDIVIDUAL Vein, iliac Cell Line
明显健康的个人细胞株
Description描述：Line HIVE-109. The iliac vein was removed from a braindead, heartbeating cadaver, organ donor on 5/26/88. The cause of death was unknown. The culture was initiated on 5/27/88 using cells released from the lumen of the vessel by collagenase digestion. Cell morphology is polygonal. Most cells were positive by immunofluorescence assay for endothelial cellspecific von Willebrand factor. Some cells were positive for alpha-smooth muscle actin (4% at PDL 16 and 6% at PDL 27). The culture was grown on gelatin-coated flasks and the medium was supplemented with 0.02 mg/ml crude ECGS and 0.05 mg/ml heparin. Cells were frozen in growth medium containing 7.5% polyvinylpyrrolidinone and 5% DMSO. The karyotype is unstable. An iliac artery endothelial cell culture from this same donor is AG10767. The legacy karyotype description shown in this Remark may not be representative of the current available product.
Category分类：Endothelial
ID编号:NTCC®-AG10768
Size/Quantity数量: 1 Flask/2 Vials
Biosafety Level生物安全级别:1
Shipping Info运输方式: RT/Dry Ice
Storage储存方式: RT/Liquid Nitrogen
Race: White
Age年龄:49 YR
Gender性别:Female
Cell Type细胞类型: APPARENTLY HEALTHY INDIVIDUAL
Affected State:No
Product:Endothelial
Gene:
Mutations突变:
Ethnicity:
Family:1256
Relationship:proband
Karyotype:47,XX,add(1)(q44),t(3;16)(p13;q22),+11[24]/46,XX,dup(1)(q25q11)[12]/46,XX,-13,+mar[4]/91,XXXX,-13[4]/47,XX,+11[2]/92,XXXX[1]/46,XX[3]
Tissue Type组织来源:Vein
Complete Growth Medium完全培养基: BioVector® Medium 199 in Earl BSS with 2mM L-glutamine or equivalent Complete Medium
Subculturing传代方法:
Cryopreservation冻存方法:Freeze medium: Complete growth medium supplemented with 5% (v/v) DMSO
Storage temperature: liquid nitrogen vapor phase
Culture Conditions培养条件:Atmosphere: air, 95%; carbon dioxide (CO2), 5%
Temperature: 37.0°C
STR Profile鉴定数据:
References参考文献:

Supplier供应商:BioVector质粒载体菌株细胞蛋白抗体基因保藏中心
NTCC典型培养物保藏中心
Tel电话：400-800-2947
Email：Biovector@163.com
http://www.biovector.net