AG09872 APPARENTLY HEALTHY INDIVIDUAL Artery, iliac Cell Line NTCC®细胞株-BioVector NTCC细胞库

Name细胞名称：NTCC® AG09872 APPARENTLY HEALTHY INDIVIDUAL Artery, iliac Cell Line
明显健康的个人细胞株
Description描述：Line HIAE-43/7-12. The iliac artery was removed from a braindead, heartbeating cadaver, renal donor. The culture was initiated using cells released from the lumen of the vessel by collagenase digestion. Cell morphology is polygonal. The culture was grown on gelatin-coated flasks and the medium was supplemented with 0.02 mg/ml ECGS and 0.05 mg/ml heparin. Cells were frozen in growth medium containing 7.5% polyvinylpyrrolidinone and 5% DMSO. The culture is a mosaic with karyotype: 46,XY/47,XY,+11/ 92,XXYY; unbalanced; 84%/6%/10%. This culture tested positive for the endothelial cell-specific von Willebrand factor and negative for alphasmooth muscle actin (immunofluorescence assays). The legacy karyotype description shown in this Remark may not be representative of the current available product.
Category分类：Endothelial
ID编号:NTCC®-AG09872
Size/Quantity数量: 1 Flask/2 Vials
Biosafety Level生物安全级别:1
Shipping Info运输方式: RT/Dry Ice
Storage储存方式: RT/Liquid Nitrogen
Race: Black/African American
Age年龄:23 YR
Gender性别:Male
Cell Type细胞类型: APPARENTLY HEALTHY INDIVIDUAL
Affected State:No
Product:Endothelial
Gene:
Mutations突变:
Ethnicity:
Family:1204
Relationship:proband
Karyotype:46,XY,dup(11)(pter>p15.1::p15.5>qter)[23]/46,XY[27]
Tissue Type组织来源:Artery
Complete Growth Medium完全培养基: BioVector® Medium 199 in Earl BSS with 2mM L-glutamine or equivalent Complete Medium
Subculturing传代方法:
Cryopreservation冻存方法:Freeze medium: Complete growth medium supplemented with 5% (v/v) DMSO
Storage temperature: liquid nitrogen vapor phase
Culture Conditions培养条件:Atmosphere: air, 95%; carbon dioxide (CO2), 5%
Temperature: 37.0°C
STR Profile鉴定数据:
References参考文献:

Supplier供应商:BioVector质粒载体菌株细胞蛋白抗体基因保藏中心
NTCC典型培养物保藏中心
Tel电话：400-800-2947
Email：Biovector@163.com
http://www.biovector.net