GM23245 THROMBOCYTHEMIA, ESSENTIAL | LEUKEMIA, ACUTE MYELOID; AML | JANUS KINASE 2; JAK2 Peripheral Vein Cell Line NTCC®细胞株-BioVector NTCC细胞库

Name细胞名称：NTCC® GM23245 THROMBOCYTHEMIA, ESSENTIAL | LEUKEMIA, ACUTE MYELOID; AML | JANUS KINASE 2; JAK2 Peripheral Vein Cell Line
血小板增多症，必不可少|白血病、急性髓系白血病；反洗钱|两面神激酶 2；贾克2细胞株
Description描述：Line UKE-1; at age 30 years subject developed carcinoma of the cervix and was treated surgically; 17 years later at age 47 she was diagnosed with essential thrombocythemia controlled with hydroxyurea without bleeding or thrombotic episodes; at age 59 she had pain and swelling of the right shoulder; exam revealed 5cm right supraclavicular mass and enlarged cervical and axillary lymph nodes; histologic exam was consistent with an epitheloid angiosarcoma of UICC grade 2; six cycles of ifosfamide and adriblastin were administerd; six months later patient presented with multiple skin infiltrations and bilateral pleural effusions; bone marrow biopsy showed infiltration with 54% blast cells; essential thrombocythemia appeared to have transformed into acute myelogenous leukemia; chemotherapy with cytosine arabinoside and mitoxanthrone did not result in regression and the patient died; this culture was derived from primary leukemic cells obtained from the patient's peripheral blood; this line appears to combine hematopoietic and endothelial features indicating the close ontogenic relation of both lineages; this donor was homozygous for a G>T transversion in the JAK2 gene resulting in the substitution of phenylalanine for valine at codon 617 [Val617Phe (V617F)]; the cell line is homozygous for the JAK2 mutation.
Category分类：Tumor-derived cell line
ID编号:NTCC®-GM23245
Size/Quantity数量: 1 Flask/2 Vials
Biosafety Level生物安全级别:1
Shipping Info运输方式: RT/Dry Ice
Storage储存方式: RT/Liquid Nitrogen
Race:
Age年龄:59 YR
Gender性别:Female
Cell Type细胞类型: THROMBOCYTHEMIA, ESSENTIAL | LEUKEMIA, ACUTE MYELOID; AML | JANUS KINASE 2; JAK2
Affected State:Yes
Product:Tumor-derived cell line
Gene:JAK2
Mutations突变:VAL617PHE
Ethnicity:
Family:
Relationship:proband
Karyotype:
Tissue Type组织来源:
Complete Growth Medium完全培养基: BioVector® Roswell Park Memorial Institute Medium 1640 with 2mM L-glutamine or equivalent Complete Medium
Subculturing传代方法:
Cryopreservation冻存方法:Freeze medium: Complete growth medium supplemented with 5% (v/v) DMSO
Storage temperature: liquid nitrogen vapor phase
Culture Conditions培养条件:Atmosphere: air, 95%; carbon dioxide (CO2), 5%
Temperature: 37.0°C
STR Profile鉴定数据:
References参考文献:

Supplier供应商:BioVector质粒载体菌株细胞蛋白抗体基因保藏中心
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