pMK289 (mAID-mClover-NeoR)哺乳动物基因编辑质粒BioVector®载体 BioVector NTCC质粒载体菌种细胞基因保藏中心

BioVector® NTCC® pMK289 (mAID-mClover-NeoR)哺乳动物基因编辑质粒BioVector®载体

pMK289 (mAID-mClover-NeoR)
(Plasmid BioVector-72827)
Purpose
C-terminal tagging with mAID-mClover using CRISPR/Cas9

Vector backbonepBluescript KS(-)
Backbone size w/o insert (bp)3242
Total vector size (bp)6117
Vector typeMammalian Expression, CRISPR
Selectable markersNeomycin (select with G418)
Growth in Bacteria
Bacterial Resistance(s)Ampicillin, 100 μg/mL
Growth Temperature37°C
Growth Strain(s)DH5alpha
Copy numberHigh Copy
Gene/Insert
Gene/Insert namemAID-mClover-Neo
SpeciesH. sapiens (human), M. musculus (mouse), R. norvegicus (rat), G. gallus (chicken)
Insert Size (bp)2875
Tag / Fusion Protein
mAID-mClover (C terminal on backbone)
Cloning Information
Cloning methodRestriction Enzyme
5′ cloning siteBamHI (not destroyed)
3′ cloning siteBamHI (not destroyed)
5′ sequencing primerGTAAAACGACGGCCAGT
3′ sequencing primerGGAAACAGCTATGACCATG

Supplier来源:BioVector NTCC Inc.
Email: biovector@163.com
Website网址: http://www.biovector.net