出品公司: | Cambia |
---|---|
载体名称: | pCambia2201, pCambia 2201 |
质粒类型: | 植物表达载体 |
高拷贝/低拷贝: | 低拷贝 |
启动子: | CAMV35S |
克隆方法: | 多克隆位点,限制性内切酶 |
载体大小: | 11786 bp |
5' 测序引物及序列: | 35S promoter: CTATCCTTCGCAAGACCCTTC |
3' 测序引物及序列: | -- |
载体标签: | GusA |
载体抗性: | 氯霉素 |
筛选标记: | NPTII |
备注: | -- |
产品目录号: | 2201 |
稳定性: | 稳定表达 |
组成型: | 非组成型 |
病毒/非病毒: | 非病毒 |
pCambia2201质粒载体图谱序列抗性价格报价Biovector NTCC Inc.现货
- 价 格:¥1920
- 货 号:BiovectorpCambia2201
- 产 地:北京
- BioVector NTCC典型培养物保藏中心
- 联系人:Dr.Xu, Biovector NTCC Inc.
电话:400-800-2947 工作QQ:1843439339 (微信同号)
邮件:Biovector@163.com
手机:18901268599
地址:北京
- 已注册
pCambia 2201载体基本信息
pCambia 2201载体质粒图谱和多克隆位点信息
pCambia 2201载体简介
pCambia 2201载体序列
LOCUS pCambia2201 11786 bp ds-DNA circular SYN 17-五月-2013 DEFINITION Agrobacterium binary vector for plant transformation, with kanamycin- and chloramphenicol-resistance and GUS genes. ACCESSION AF234314 VERSION . KEYWORDS pCAMBIA2201 SOURCE synthetic DNA construct ORGANISM synthetic DNA construct COMMENT This file is created by Vector NTI http://www.biovector.net/ COMMENT VNTAUTHORNAME|biovector.net| COMMENT The GenBank record was corrected by inserting a G at position 4743. FEATURES Location/Qualifiers source 1..11786 /organism="synthetic DNA construct" /lab_host="Plant Cell" /mol_type="other DNA" CDS join(2..16,207..2024) /codon_start=1 /product="beta-glucuronidase" /label="GUS" /label="This version of the gusA gene has a 5' extension with a catalase intron to ensure expression in plants but not bacteria." /protein_id=" " intron 17..206 /label="cat1 intron" /label="castor bean catalase intron, modified" CDS 2031..2048 /codon_start=1 /product="6xHis affinity tag" /label="6xHis" /translation="HHHHHH" terminator 2083..2335 /label="NOS terminator" /label="nopaline synthase terminator and poly(A) signal" misc_feature 2357..2381 /label="RB T-DNA repeat" /label="right border repeat from nopaline C58 T-DNA" CDS 3681..4310 /codon_start=1 /product="stability protein from Pseudomonas plasmid pVS1" /label="pVS1 StaA" CDS 4739..5812 /codon_start=1 /product="replication protein from Pseudomonas plasmid pVS1" /label="pVS1 RepA" /label=" " /protein_id=" " misc_feature 6416..6556 /label="bom" /label="basis of mobility region from pBR322" rep_origin complement(6742..7330) /direction=LEFT /label="ori" /label="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication" CDS complement(7601..8260) /codon_start=1 /gene="cat" /product="chloramphenicol acetyltransferase" /label="CmR" /label="confers resistance to chloramphenicol" misc_feature 8788..8812 /label="LB T-DNA repeat" /label="left border repeat from nopaline C58 T-DNA" polyA_signal 8890..9064 /label="CaMV poly(A) signal" /label="cauliflower mosaic virus polyadenylation signal" CDS complement(9121..9918) /codon_start=1 /gene="aph(3')-II (or nptII)" /product="aminoglycoside phosphotransferase from Tn5" /label="NeoR/KanR" /label="confers resistance to neomycin, kanamycin, and G418 (Geneticin(R))" promoter complement(9981..10658) /label="CaMV 35S promoter (enhanced)" /label="cauliflower mosaic virus 35S promoter with a duplicated enhancer region" promoter 10885..10915 /label="lac promoter" /label="promoter for the E. coli lac operon" protein_bind 10923..10939 /bound_moiety="lac repressor encoded by lacI" /label="lac operator" /label="The lac repressor binds to the lac operator to inhibit transcription in E. coli. This inhibition can be relieved by adding lactose or isopropyl-beta-D-thiogalactopyranoside (IPTG)." primer_bind 10947..10963 /label="M13 rev" /label="common sequencing primer, one of multiple similar variants" CDS 10959..11192 /codon_start=1 /gene="lacZ (truncated)" /product="LacZ-alpha fragment of?beta-galactosidase" /label="lacZ-alpha" /protein_id=" " misc_feature 10973..11029 /gene=" " /label="MCS" /label="pUC18/19 multiple cloning site" primer_bind complement(11033..11049) /label="M13 fwd" /label="common sequencing primer, one of multiple similar variants" promoter 11426..11771 /label="CaMV 35S promoter" /label="strong constitutive promoter from cauliflower mosaic virus" ORIGIN 1 catggtagat ctgagggtaa atttctagtt tttctccttc attttcttgg ttaggaccct 61 tttctctttt tatttttttg agctttgatc tttctttaaa ctgatctatt ttttaattga 121 ttggttatgg tgtaaatatt acatagcttt aactgataat ctgattactt tatttcgtgt 181 gtctatgatg atgatgatag ttacagaacc gacgactcgt ccgtcctgta gaaaccccaa 241 cccgtgaaat caaaaaactc gacggcctgt gggcattcag tctggatcgc gaaaactgtg 301 gaattgatca gcgttggtgg gaaagcgcgt tacaagaaag ccgggcaatt gctgtgccag 361 gcagttttaa cgatcagttc gccgatgcag atattcgtaa ttatgcgggc aacgtctggt 421 atcagcgcga agtctttata ccgaaaggtt gggcaggcca gcgtatcgtg ctgcgtttcg 481 atgcggtcac tcattacggc aaagtgtggg tcaataatca ggaagtgatg gagcatcagg 541 gcggctatac gccatttgaa gccgatgtca cgccgtatgt tattgccggg aaaagtgtac 601 gtatcaccgt ttgtgtgaac aacgaactga actggcagac tatcccgccg ggaatggtga 661 ttaccgacga aaacggcaag aaaaagcagt cttacttcca tgatttcttt aactatgccg 721 gaatccatcg cagcgtaatg ctctacacca cgccgaacac ctgggtggac gatatcaccg 781 tggtgacgca tgtcgcgcaa gactgtaacc acgcgtctgt tgactggcag gtggtggcca 841 atggtgatgt cagcgttgaa ctgcgtgatg cggatcaaca ggtggttgca actggacaag 901 gcactagcgg gactttgcaa gtggtgaatc cgcacctctg gcaaccgggt gaaggttatc 961 tctatgaact cgaagtcaca gccaaaagcc agacagagtc tgatatctac ccgcttcgcg 1021 tcggcatccg gtcagtggca gtgaagggcc aacagttcct gattaaccac aaaccgttct 1081 actttactgg ctttggtcgt catgaagatg cggacttacg tggcaaagga ttcgataacg 1141 tgctgatggt gcacgaccac gcattaatgg actggattgg ggccaactcc taccgtacct 1201 cgcattaccc ttacgctgaa gagatgctcg actgggcaga tgaacatggc atcgtggtga 1261 ttgatgaaac tgctgctgtc ggctttcagc tgtctttagg cattggtttc gaagcgggca 1321 acaagccgaa agaactgtac agcgaagagg cagtcaacgg ggaaactcag caagcgcact 1381 tacaggcgat taaagagctg atagcgcgtg acaaaaacca cccaagcgtg gtgatgtgga 1441 gtattgccaa cgaaccggat acccgtccgc aaggtgcacg ggaatatttc gcgccactgg 1501 cggaagcaac gcgtaaactc gacccgacgc gtccgatcac ctgcgtcaat gtaatgttct 1561 gcgacgctca caccgatacc atcagcgatc tctttgatgt gctgtgcctg aaccgttatt 1621 acggatggta tgtccaaagc ggcgatttgg aaacggcaga gaaggtactg gaaaaagaac 1681 ttctggcctg gcaggagaaa ctgcatcagc cgattatcat caccgaatac ggcgtggata 1741 cgttagccgg gctgcactca atgtacaccg acatgtggag tgaagagtat cagtgtgcat 1801 ggctggatat gtatcaccgc gtctttgatc gcgtcagcgc cgtcgtcggt gaacaggtat 1861 ggaatttcgc cgattttgcg acctcgcaag gcatattgcg cgttggcggt aacaagaaag 1921 ggatcttcac tcgcgaccgc aaaccgaagt cggcggcttt tctgctgcaa aaacgctgga 1981 ctggcatgaa cttcggtgaa aaaccgcagc agggaggcaa acaagctagc caccaccacc 2041 accaccacgt gtgaattaca ggtgaccagc tcgaatttcc ccgatcgttc aaacatttgg 2101 caataaagtt tcttaagatt gaatcctgtt gccggtcttg cgatgattat catataattt 2161 ctgttgaatt acgttaagca tgtaataatt aacatgtaat gcatgacgtt atttatgaga 2221 tgggttttta tgattagagt cccgcaatta tacatttaat acgcgataga aaacaaaata 2281 tagcgcgcaa actaggataa attatcgcgc gcggtgtcat ctatgttact agatcgggaa 2341 ttaaactatc agtgtttgac aggatatatt ggcgggtaaa cctaagagaa aagagcgttt 2401 attagaataa cggatattta aaagggcgtg aaaaggttta tccgttcgtc catttgtatg 2461 tgcatgccaa ccacagggtt cccctcggga tcaaagtact ttgatccaac ccctccgctg 2521 ctatagtgca gtcggcttct gacgttcagt gcagccgtct tctgaaaacg acatgtcgca 2581 caagtcctaa gttacgcgac aggctgccgc cctgcccttt tcctggcgtt ttcttgtcgc 2641 gtgttttagt cgcataaagt agaatacttg cgactagaac cggagacatt acgccatgaa 2701 caagagcgcc gccgctggcc tgctgggcta tgcccgcgtc agcaccgacg accaggactt 2761 gaccaaccaa cgggccgaac tgcacgcggc cggctgcacc aagctgtttt ccgagaagat 2821 caccggcacc aggcgcgacc gcccggagct ggccaggatg cttgaccacc tacgccctgg 2881 cgacgttgtg acagtgacca ggctagaccg cctggcccgc agcacccgcg acctactgga 2941 cattgccgag cgcatccagg aggccggcgc gggcctgcgt agcctggcag agccgtgggc 3001 cgacaccacc acgccggccg gccgcatggt gttgaccgtg ttcgccggca ttgccgagtt 3061 cgagcgttcc ctaatcatcg accgcacccg gagcgggcgc gaggccgcca aggcccgagg 3121 cgtgaagttt ggcccccgcc ctaccctcac cccggcacag atcgcgcacg cccgcgagct 3181 gatcgaccag gaaggccgca ccgtgaaaga ggcggctgca ctgcttggcg tgcatcgctc 3241 gaccctgtac cgcgcacttg agcgcagcga ggaagtgacg cccaccgagg ccaggcggcg 3301 cggtgccttc cgtgaggacg cattgaccga ggccgacgcc ctggcggccg ccgagaatga 3361 acgccaagag gaacaagcat gaaaccgcac caggacggcc aggacgaacc gtttttcatt 3421 accgaagaga tcgaggcgga gatgatcgcg gccgggtacg tgttcgagcc gcccgcgcac 3481 gtctcaaccg tgcggctgca tgaaatcctg gccggtttgt ctgatgccaa gctggcggcc 3541 tggccggcca gcttggccgc tgaagaaacc gagcgccgcc gtctaaaaag gtgatgtgta 3601 tttgagtaaa acagcttgcg tcatgcggtc gctgcgtata tgatgcgatg agtaaataaa 3661 caaatacgca aggggaacgc atgaaggtta tcgctgtact taaccagaaa ggcgggtcag 3721 gcaagacgac catcgcaacc catctagccc gcgccctgca actcgccggg gccgatgttc 3781 tgttagtcga ttccgatccc cagggcagtg cccgcgattg ggcggccgtg cgggaagatc 3841 aaccgctaac cgttgtcggc atcgaccgcc cgacgattga ccgcgacgtg aaggccatcg 3901 gccggcgcga cttcgtagtg atcgacggag cgccccaggc ggcggacttg gctgtgtccg 3961 cgatcaaggc agccgacttc gtgctgattc cggtgcagcc aagcccttac gacatatggg 4021 ccaccgccga cctggtggag ctggttaagc agcgcattga ggtcacggat ggaaggctac 4081 aagcggcctt tgtcgtgtcg cgggcgatca aaggcacgcg catcggcggt gaggttgccg 4141 aggcgctggc cgggtacgag ctgcccattc ttgagtcccg tatcacgcag cgcgtgagct 4201 acccaggcac tgccgccgcc ggcacaaccg ttcttgaatc agaacccgag ggcgacgctg 4261 cccgcgaggt ccaggcgctg gccgctgaaa ttaaatcaaa actcatttga gttaatgagg 4321 taaagagaaa atgagcaaaa gcacaaacac gctaagtgcc ggccgtccga gcgcacgcag 4381 cagcaaggct gcaacgttgg ccagcctggc agacacgcca gccatgaagc gggtcaactt 4441 tcagttgccg gcggaggatc acaccaagct gaagatgtac gcggtacgcc aaggcaagac 4501 cattaccgag ctgctatctg aatacatcgc gcagctacca gagtaaatga gcaaatgaat 4561 aaatgagtag atgaatttta gcggctaaag gaggcggcat ggaaaatcaa gaacaaccag 4621 gcaccgacgc cgtggaatgc cccatgtgtg gaggaacggg cggttggcca ggcgtaagcg 4681 gctgggttgt ctgccggccc tgcaatggca ctggaacccc caagcccgag gaatcggcgt 4741 gagcggtcgc aaaccatccg gcccggtaca aatcggcgcg gcgctgggtg atgacctggt 4801 ggagaagttg aaggccgcgc aggccgccca gcggcaacgc atcgaggcag aagcacgccc 4861 cggtgaatcg tggcaagcgg ccgctgatcg aatccgcaaa gaatcccggc aaccgccggc 4921 agccggtgcg ccgtcgatta ggaagccgcc caagggcgac gagcaaccag attttttcgt 4981 tccgatgctc tatgacgtgg gcacccgcga tagtcgcagc atcatggacg tggccgtttt 5041 ccgtctgtcg aagcgtgacc gacgagctgg cgaggtgatc cgctacgagc ttccagacgg 5101 gcacgtagag gtttccgcag ggccggccgg catggccagt gtgtgggatt acgacctggt 5161 actgatggcg gtttcccatc taaccgaatc catgaaccga taccgggaag ggaagggaga 5221 caagcccggc cgcgtgttcc gtccacacgt tgcggacgta ctcaagttct gccggcgagc 5281 cgatggcgga aagcagaaag acgacctggt agaaacctgc attcggttaa acaccacgca 5341 cgttgccatg cagcgtacga agaaggccaa gaacggccgc ctggtgacgg tatccgaggg 5401 tgaagccttg attagccgct acaagatcgt aaagagcgaa accgggcggc cggagtacat 5461 cgagatcgag ctagctgatt ggatgtaccg cgagatcaca gaaggcaaga acccggacgt 5521 gctgacggtt caccccgatt actttttgat cgatcccggc atcggccgtt ttctctaccg 5581 cctggcacgc cgcgccgcag gcaaggcaga agccagatgg ttgttcaaga cgatctacga 5641 acgcagtggc agcgccggag agttcaagaa gttctgtttc accgtgcgca agctgatcgg 5701 gtcaaatgac ctgccggagt acgatttgaa ggaggaggcg gggcaggctg gcccgatcct 5761 agtcatgcgc taccgcaacc tgatcgaggg cgaagcatcc gccggttcct aatgtacgga 5821 gcagatgcta gggcaaattg ccctagcagg ggaaaaaggt cgaaaaggtc tctttcctgt 5881 ggatagcacg tacattggga acccaaagcc gtacattggg aaccggaacc cgtacattgg 5941 gaacccaaag ccgtacattg ggaaccggtc acacatgtaa gtgactgata taaaagagaa 6001 aaaaggcgat ttttccgcct aaaactcttt aaaacttatt aaaactctta aaacccgcct 6061 ggcctgtgca taactgtctg gccagcgcac agccgaagag ctgcaaaaag cgcctaccct 6121 tcggtcgctg cgctccctac gccccgccgc ttcgcgtcgg cctatcgcgg ccgctggccg 6181 ctcaaaaatg gctggcctac ggccaggcaa tctaccaggg cgcggacaag ccgcgccgtc 6241 gccactcgac cgccggcgcc cacatcaagg caccctgcct cgcgcgtttc ggtgatgacg 6301 gtgaaaacct ctgacacatg cagctcccgg agacggtcac agcttgtctg taagcggatg 6361 ccgggagcag acaagcccgt cagggcgcgt cagcgggtgt tggcgggtgt cggggcgcag 6421 ccatgaccca gtcacgtagc gatagcggag tgtatactgg cttaactatg cggcatcaga 6481 gcagattgta ctgagagtgc accatatgcg gtgtgaaata ccgcacagat gcgtaaggag 6541 aaaataccgc atcaggcgct cttccgcttc ctcgctcact gactcgctgc gctcggtcgt 6601 tcggctgcgg cgagcggtat cagctcactc aaaggcggta atacggttat ccacagaatc 6661 aggggataac gcaggaaaga acatgtgagc aaaaggccag caaaaggcca ggaaccgtaa 6721 aaaggccgcg ttgctggcgt ttttccatag gctccgcccc cctgacgagc atcacaaaaa 6781 tcgacgctca agtcagaggt ggcgaaaccc gacaggacta taaagatacc aggcgtttcc 6841 ccctggaagc tccctcgtgc gctctcctgt tccgaccctg ccgcttaccg gatacctgtc 6901 cgcctttctc ccttcgggaa gcgtggcgct ttctcatagc tcacgctgta ggtatctcag 6961 ttcggtgtag gtcgttcgct ccaagctggg ctgtgtgcac gaaccccccg ttcagcccga 7021 ccgctgcgcc ttatccggta actatcgtct tgagtccaac ccggtaagac acgacttatc 7081 gccactggca gcagccactg gtaacaggat tagcagagcg aggtatgtag gcggtgctac 7141 agagttcttg aagtggtggc ctaactacgg ctacactaga aggacagtat ttggtatctg 7201 cgctctgctg aagccagtta ccttcggaaa aagagttggt agctcttgat ccggcaaaca 7261 aaccaccgct ggtagcggtg gtttttttgt ttgcaagcag cagattacgc gcagaaaaaa 7321 aggatctcaa gaagatcctt tgatcttttc tacggggtct gacgctcagt ggaacgaaaa 7381 ctcacgttaa gggattttgg tcatgcatga tatatctccc aatttgtgta gggcttatta 7441 tgcacgctta aaaataataa aagcagactt gacctgatag tttggctgtg agcaattatg 7501 tgcttagtgc atctaatcgc ttgagttaac gccggcgaag cggcgtcggc ttgaacgaat 7561 ttctagctag aggatcgcac caataactgc cttaaaaaaa ttacgccccg ccctgccact 7621 catcgcagta ctgttgtaat tcattaagca ttctgccgac atggaagcca tcacaaacgg 7681 catgatgaac ctgaatcgcc agcggcatca gcaccttgtc gccttgcgta taatatttgc 7741 ccattgtgaa aacgggggcg aagaagttgt ccatattggc cacgtttaaa tcaaaactgg 7801 tgaaactcac ccagggattg gctgagacga aaaacatatt ctcaataaac cctttaggga 7861 aataggccag gttttcaccg taacacgcca catcttgcga atatatgtgt agaaactgcc 7921 ggaaatcgtc gtggtattca ctccagagcg atgaaaacgt ttcagtttgc tcatggaaaa 7981 cggtgtaaca agggtgaaca ctatcccata tcaccagctc accgtctttc attgccatac 8041 ggaactccgg atgagcattc atcaggcggg caagaatgtg aataaaggcc ggataaaact 8101 tgtgcttatt tttctttacg gtctttaaaa aggccgtaat atccagctga acggtctggt 8161 tataggtaca ttgagcaact gactgaaatg cctcaaaatg ttctttacga tgccattggg 8221 atatatcaac ggtggtatat ccagtgattt ttttctccat gatgtttaac tttgttttag 8281 ggcgactgcc ctgctgcgta acatcgttgc tgctccataa catcaaacat cgacccacgg 8341 cgtaacgcgc ttgctgcttg gatgcccgag gcatagactg taccccaaaa aaacatgtca 8401 taacaagaag ccatgaaaac cgccactgcg ccgttaccac cgctgcgttc ggtcaaggtt 8461 ctggaccagt tgcgtgacgg cagttacgct acttgcatta cagcttacga accgaacgag 8521 gcttatgtcc actgggttcg tgcccgaatt gatcacaggc agcaacgctc tgtcatcgtt 8581 acaatcaaca tgctaccctc cgcgagatca tccgtgtttc aaacccggca gcttagttgc 8641 cgttcttccg aatagcatcg gtaacatgag caaagtctgc cgccttacaa cggctctccc 8701 gctgacgccg tcccggactg atgggctgcc tgtatcgagt ggtgattttg tgccgagctg 8761 ccggtcgggg agctgttggc tggctggtgg caggatatat tgtggtgtaa acaaattgac 8821 gcttagacaa cttaataaca cattgcggac gtttttaatg tactgaatta acgccgaatt 8881 aattcggggg atctggattt tagtactgga ttttggtttt aggaattaga aattttattg 8941 atagaagtat tttacaaata caaatacata ctaagggttt cttatatgct caacacatga 9001 gcgaaaccct ataggaaccc taattccctt atctgggaac tactcacaca ttattatgga 9061 gaaactcgag cttgtcgatc gactctagct agaggatcga tccgaacccc agagtcccgc 9121 tcagaagaac tcgtcaagaa ggcgatagaa ggcgatgcgc tgcgaatcgg gagcggcgat 9181 accgtaaagc acgaggaagc ggtcagccca ttcgccgcca agctcttcag caatatcacg 9241 ggtagccaac gctatgtcct gatagcggtc cgccacaccc agccggccac agtcgatgaa 9301 tccagaaaag cggccatttt ccaccatgat attcggcaag caggcatcgc catgtgtcac 9361 gacgagatcc tcgccgtcgg gcatgcgcgc cttgagcctg gcgaacagtt cggctggcgc 9421 gagcccctga tgctcttcgt ccagatcatc ctgatcgaca agaccggctt ccatccgagt 9481 acgtgctcgc tcgatgcgat gtttcgcttg gtggtcgaat gggcaggtag ccggatcaag 9541 cgtatgcagc cgccgcattg catcagccat gatggatact ttctcggcag gagcaaggtg 9601 agatgacagg agatcctgcc ccggcacttc gcccaatagc agccagtccc ttcccgcttc 9661 agtgacaacg tcgagcacag ctgcgcaagg aacgcccgtc gtggccagcc acgatagccg 9721 cgctgcctcg tcctggagtt cattcagggc accggacagg tcggtcttga caaaaagaac 9781 cgggcgcccc tgcgctgaca gccggaacac ggcggcatca gagcagccga ttgtctgttg 9841 tgcccagtca tagccgaata gcctctccac ccaagcggcc ggagaacctg cgtgcaatcc 9901 atcttgttca atccccatgg tcgatcgaca gatctgcgaa agctcgagag agatagattt 9961 gtagagagag actggtgatt tcagcgtgtc ctctccaaat gaaatgaact tccttatata 10021 gaggaaggtc ttgcgaagga tagtgggatt gtgcgtcatc ccttacgtca gtggagatat 10081 cacatcaatc cacttgcttt gaagacgtgg ttggaacgtc ttctttttcc acgatgctcc 10141 tcgtgggtgg gggtccatct ttgggaccac tgtcggcaga ggcatcttga acgatagcct 10201 ttcctttatc gcaatgatgg catttgtagg tgccaccttc cttttctact gtccttttga 10261 tgaagtgaca gatagctggg caatggaatc cgaggaggtt tcccgatatt accctttgtt 10321 gaaaagtctc aatagccctt tggtcttctg agactgtatc tttgatattc ttggagtaga 10381 cgagagtgtc gtgctccacc atgttatcac atcaatccac ttgctttgaa gacgtggttg 10441 gaacgtcttc tttttccacg atgctcctcg tgggtggggg tccatctttg ggaccactgt 10501 cggcagaggc atcttgaacg atagcctttc ctttatcgca atgatggcat ttgtaggtgc 10561 caccttcctt ttctactgtc cttttgatga agtgacagat agctgggcaa tggaatccga 10621 ggaggtttcc cgatattacc ctttgttgaa aagtctcaat agccctttgg tcttctgaga 10681 ctgtatcttt gatattcttg gagtagacga gagtgtcgtg ctccaccatg ttggcaagct 10741 gctctagcca atacgcaaac cgcctctccc cgcgcgttgg ccgattcatt aatgcagctg 10801 gcacgacagg tttcccgact ggaaagcggg cagtgagcgc aacgcaatta atgtgagtta 10861 gctcactcat taggcacccc aggctttaca ctttatgctt ccggctcgta tgttgtgtgg 10921 aattgtgagc ggataacaat ttcacacagg aaacagctat gaccatgatt acgaattcga 10981 gctcggtacc cggggatcct ctagagtcga cctgcaggca tgcaagcttg gcactggccg 11041 tcgttttaca acgtcgtgac tgggaaaacc ctggcgttac ccaacttaat cgccttgcag 11101 cacatccccc tttcgccagc tggcgtaata gcgaagaggc ccgcaccgat cgcccttccc 11161 aacagttgcg cagcctgaat ggcgaatgct agagcagctt gagcttggat cagattgtcg 11221 tttcccgcct tcagtttagc ttcatggagt caaagattca aatagaggac ctaacagaac 11281 tcgccgtaaa gactggcgaa cagttcatac agagtctctt acgactcaat gacaagaaga 11341 aaatcttcgt caacatggtg gagcacgaca cacttgtcta ctccaaaaat atcaaagata 11401 cagtctcaga agaccaaagg gcaattgaga cttttcaaca aagggtaata tccggaaacc 11461 tcctcggatt ccattgccca gctatctgtc actttattgt gaagatagtg gaaaaggaag 11521 gtggctccta caaatgccat cattgcgata aaggaaaggc catcgttgaa gatgcctctg 11581 ccgacagtgg tcccaaagat ggacccccac ccacgaggag catcgtggaa aaagaagacg 11641 ttccaaccac gtcttcaaag caagtggatt gatgtgatat ctccactgac gtaagggatg 11701 acgcacaatc ccactatcct tcgcaagacc cttcctctat ataaggaagt tcatttcatt 11761 tggagagaac acgggggact cttgac
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