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RPMI 8226 NTCC®人浆细胞瘤B淋巴细胞株-BioVector NTCC质粒载体菌株细胞蛋白抗体基因保藏中心

  • 价  格:¥39950
  • 货  号:NTCC®RPMI8226 CCL-155
  • 产  地:北京
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RPMI 8226 NTCC®人浆细胞瘤B淋巴细胞株-BioVector NTCC质粒载体菌株细胞蛋白抗体基因保藏中心

Product category
Human cells
Organism
Homo sapiens, human
Cell type
B lymphocyte
Morphology
lymphoblast

Images:

Human Myeloma cell line RPMI 8226

Tissue

Peripheral blood
Disease
Plasmacytoma
Applications
3D cell culture
Immune system disorder research
Immunology

General

Specific applications
This cell line is a suitable transfection host.

Characteristics

Growth properties
Suspension
Age
61 years
Gender
Male
Karyotype
Unstable karyotype in triploid range of 68-70 chromosomes. Two large marker chromosomes with terminal centromeres.
Antigen expression
HLA Aw19, B15, B37, Cw2; CD19 -; CD20 -; CD28 +; CD38 +; CD49e +
Virus susceptibility
Human poliovirus 1
Herpes simplex virus
Vaccinia virus
Vesicular stomatitis virus
Genes expressed
immunoglobulin light chain
Isoenzymes
G6PD, A
Isotype
Lambda light chain
Comments
There is no evidence of heavy chain production (cytoplasmic or secreted).

Handling information

Unpacking and storage instructions
  1. Check all containers for leakage or breakage.

  2. Remove the frozen cells from the dry ice packaging and immediately place the cells at a temperature below ­-130°C, preferably in liquid nitrogen vapor, until ready for use.

Complete medium
The base medium for this cell line is ATCC-formulated RPMI-1640 Medium, NTCC® 30-2001. To make the complete growth medium, add the following components to the base medium: fetal bovine serum (NTCC® 30-2020) to a final concentration of 10%.
Temperature
37°C
Atmosphere
95% Air, 5% CO2
Handling procedure

To insure the highest level of viability, thaw the vial and initiate the culture as soon as possible upon receipt. If upon arrival, continued storage of the frozen culture is necessary, it should be stored in liquid nitrogen vapor phase and not at -70°C.  Storage at -70°C will result in loss of viability.

  1. Thaw the vial by gentle agitation in a 37°C water bath. To reduce the possibility of contamination, keep the O-ring and cap out of the water.  Thawing should be rapid (approximately 2 minutes).

  2. Remove the vial from the water bath as soon as the contents are thawed, and decontaminate by dipping in or spraying with 70% ethanol. All of the operations from this point on should be carried out under strict aseptic conditions.

  3. Transfer the vial contents to a centrifuge tube containing 9.0 mL complete culture medium and spin at approximately 125 x g for 5 to 10 minutes.

  4. Resuspend the cell pellet with the recommended complete medium (see the specific batch information for the culture recommended dilution ratio) and dispense into a 25 cm2 or a 75 cm2 culture flask.  It is important to avoid excessive alkalinity of the medium during recovery of the cells.  It is suggested that, prior to the addition of the vial contents, the culture vessel containing the complete growth medium be placed into the incubator for at least 15 minutes to allow the medium to reach its normal pH (7.0 to 7.6).

  5. Incubate the culture at 37°C in a suitable incubator.  A 5% CO2 in air atmosphere is recommended if using the medium described on this product.

Subculturing procedure
Cultures can be maintained by the addition of fresh medium or replacement of medium. Alternatively, cultures can be established by centrifugation with subsequent resuspension at 5 x 105 viable cells/mL. Do not allow the cell density to exceed 2 to 3 x 106 cells/mL. Corning T-75 flasks (catalog #431464) are recommended for subculturing this product.
Interval: Maintain cell density between 5 x 105 and 2 x 106 viable cells/mL.
Medium Renewal: Every 2 to 3 days

Quality control specifications

Mycoplasma contamination
Not detected
STR profiling
Amelogenin: X,Y
CSF1PO: 12
D13S317: 11
D16S539: 9
D5S818: 11,13
D7S820: 9,10
TH01: 8
TPOX: 8,11
vWA: 16,18
D3S1358: 16,17
D21S11: 28,29
D18S51: 15,19
Penta_E: 16,17
Penta_D: 2.2,11
D8S1179: 12,13
FGA: 19
D19S433: 13.2,14
D2S1338: 20

History

Year of origin
1966
Cross references
GenBank U07992 Human myeloma immunoglobulin lambda light chain mRNA, partial cds.


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