L-1236人源霍奇金淋巴瘤细胞株NTCC® Human Hodgkin lymphoma cell lineBioVector NTCC质粒载体菌株细胞蛋白抗体基因保藏中心

human (Homo sapiens)

Hodgkin lymphoma

established from the peripheral blood of a 34-year-old man with Hodgkin lymphoma (mixed cellularity, stage IV, refractory, terminal, third relapse) in 1994. Exome and RNA sequence data are available (see Ref 18187 and Exome sequence and RNA-Seq)

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<a href="https://www.dsmz.de/collection/catalogue/human-and-animal-cell-lines/permissions-/-restrictions" "="" class="internal-link-new-window" style="box-sizing: border-box; color: rgb(48, 76, 178); text-decoration-line: none;">A

variable morphology with semi-adherent spheroid to fusiform cells growing in clumps in mono- and multilayers, occasional giant cells; image; image; image; image; image; image

80-90% RPMI 1640 + 10-20% h.i. FBS

seed out at ca. 0.5 x 10⁶ cells/ml; maintain at ca. 0.3-0.5 x 10⁶ cells/ml; split saturated culture 1:2 to 1:3 every 2-3 days, adherent cells can be detached by tapping the flask; maximal density of ca. 0.6 x 10⁶ cells/ml; a daily exchange of spent medium might be of advantage

at 37 °C with 5% CO₂

ca. 48 hours

cell harvest of ca. 0.6 x 10⁶ cells/ml

frozen with 70% medium, 20% FBS, 10% DMSO

negative in microbiological culture, PCR assays

CD2 -, CD 3 -, CD13 -, CD14 -, CD15 +, CD19 -, CD25 -, CD30 +, CD54 +, CD80 +; image

STR analysis according to the global standard ANSI/ATCC ASN-0002.1-2021 (2021) resulted in an authentic STR profile of the reference STR database.

human flat-moded hypotriploid karyotype with 17% polyploidy - 57-66<3n>XX, -Y, +3, -4, -10, +11, -14, -18, +19, -20, -21, -22, +4-6mar, add(1)(p22), t(1;14)(p34 ;q22), der(1;9)(q10;p10), der(1)add(1)(p34)dup(1)(q21q43), der(1)add(1)(p2?)del(1)(q21), add(2)(q ?22), inv dup(2)(p16p23)x2, der(3)t(3;9)(q27;q21), add(6)(p23), add(6)(q22-24), add(7)(p22), der(7)t(7;7)(p22;q22), inv(7)(p11q35)add(7)(p11), add(8)(q24), der(8)t(1;8)(p32;q24)t(1;14)(p34;q22), del(9)(q21), del(10)(q12), add(11)(p1 ?), del(11)(q13), der(12)t(12;17)(p13;q13)t(17;Y)(q22;q11), del(12)(q21-22), add(13)(p11), inv(14)(p13q32.32)del(14)(q22;q32.32)x2, t(15;20)(q22;p11-12), add(16)(q23-24), del(17)(q23)x2, add(19)(q13) - resembles originator's karyotype

PCR: EBV -, HBV -, HCV -, HIV-1 -, HIV-2 -, HTLV-1/2 -, MLV -, SMRV -