40LB NTCC®永生化小鼠成纤维细胞株mouse fibroblast cell line-BioVector NTCC质粒载体菌株细胞蛋白抗体基因保藏中心

40LB NTCC®永生化小鼠成纤维细胞株mouse fibroblast cell line-BioVector NTCC质粒载体菌株细胞蛋白抗体基因保藏中心

NTCC® 40LB cell refers to a specific type of cell line used in biological research, particularly in immunology.

Origin and Purpose:

• Murine Fibroblast Cell Line: 40LB cells are derived from mouse fibroblasts, which are cells that make up connective tissue.

• CD40L and BAFF Expression: These cells are engineered to express two important molecules:

  • CD40L (CD40 Ligand): A protein that stimulates B cells, a type of immune cell responsible for producing antibodies.

  • BAFF (B-cell activating factor): A survival factor for B cells.

  
  

• Mimicking T-cell Interaction: 40LB cells are used to mimic the interaction between B cells and T helper cells, which is crucial for B cell activation and antibody production. This is particularly relevant in studying germinal centers, which are sites in lymph nodes where B cells mature and differentiate.

Applications in Research:

• In vitro B cell studies: 40LB cells are commonly used in laboratory experiments to study B cell behavior, such as proliferation, differentiation into antibody-secreting cells, and the formation of memory B cells.

• Germinal center research: These cells help researchers understand the complex processes that occur within germinal centers, which are essential for generating effective immune responses.

• Vaccine development: By studying B cell responses using 40LB cells, scientists can gain insights into how to design better vaccines that elicit strong and long-lasting antibody production.

Key Characteristics:

• Feeder cells: 40LB cells are often used as "feeder cells," meaning they provide a supportive environment for culturing other cells, such as B cells.

• Stable expression: These cells are engineered to stably express CD40L and BAFF, ensuring consistent stimulation of B cells in experiments.

Important Notes:

• Research use only: 40LB cells are typically used for research purposes in laboratory settings and are not intended for direct use in humans or animals.

• Specific protocols: The use of 40LB cells often involves specific culture conditions and experimental protocols that need to be followed carefully.

Another name	40LB-NB
Original cell	BALB/3T3 A31
Cloning (depositor)	No
Animal	_mouse < Mammals
Strain name	BALB/c
Genus	Mus
Species	musculus
Gender	Unknown
Tissue	embryo
Classification	transformed
Recombinant	recombinant
Exogene	mouse CD40L cDNA in pApuro2, mouse BAFF cDNA in pCA-neo
Vector	pApuro2, pCA-neo
Selection	puromycin (2.5 μg/ml), G418 (0.5 mg/ml)
Recombinant virus	No
	Lifespan	infinite
	Morphology	fibroblast-like
	Cellosaurus(Expasy)	CVCL_A6ZS
			deposit info	lot info
		Medium
		Culture type	Adherent cells	Adherent cells
		Culture medium	DMEM (high glucose, WAKO #044-29765) + 10% FBS (heat-inactivated)	DMEM(high glucose,without sodium pyruvate) + 10% FBS (heat inactivated)
		Antibiotics	100 U/ml penicillin, 100 μg/ml streptomycin	Free
		Passage method		0.25% Trypsin
	Culture information	Passage cell No	3 x 10 6 /φ10 cm dish
		Passage ratio		1 : 6 split
		SC frequency	Passage the cells every 3 days with 6-fold dilutions	Subculture : 2 times/week
		Temperature	37 ℃	37 ℃
		CO2 concentration	5 %	5 %
		Freeze medium	DMEM + 20% FBS + 10% DMSO	DMEM(high glucose,without sodium pyruvate) + 20% FBS (heat inactivated) + 10% DMSO
		Freezing method		Slow freezing
		Mycoplasma/Acholeplasma		(-)
		Mycoplasma	(-)
		Animal PCR		OK
		SSLP(mouse)		OK
Images			deposit info	lot info