Akata-eGFP-EBV NTCC®人源伯基特淋巴瘤细胞EBV荧光病毒侵染株Burkitt’s lymphoma cell line-BioVector NTCC Inc.
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Akata-eGFP-EBV NTCC®人源伯基特淋巴瘤细胞EBV荧光病毒侵染株Burkitt’s lymphoma cell line-BioVector NTCC Inc.
Akata-eGFP-EBV cell is a specific type of cell line used in research to study Epstein-Barr virus (EBV) infection and its role in diseases like Burkitt's lymphoma. Here's a breakdown:
Akata cells: These are derived from a Burkitt's lymphoma cell line. Burkitt's lymphoma is a type of cancer that affects B-cells, a part of your immune system.
eGFP: This stands for enhanced green fluorescent protein. Scientists have inserted the gene for this protein into the EBV that infects these cells. This makes the infected cells glow green under certain light, allowing researchers to easily visualize and track the infection.
EBV: This is the Epstein-Barr virus, a common human virus that can cause various illnesses, including mononucleosis (mono) and certain cancers like Burkitt's lymphoma.
How it's used in research:
By using Akata cells infected with eGFP-tagged EBV, scientists can:
Study EBV infection: Observe how the virus infects cells, replicates, and interacts with the host cell's machinery.
Investigate EBV-related diseases: Understand how EBV contributes to the development of cancers like Burkitt's lymphoma.
Develop antiviral therapies: Test the effectiveness of new drugs that target EBV infection.
Profile | A clone in which Akata-EBV-negative cells were reinfected with recombinant EBV in which GFP was integrated into the genome. | Other Name | |
---|---|---|---|
Animal | human | Strain | |
Genus | Homo | Species | sapiens |
Sex | F | Age | 4-year-old |
Identity | not done | Tissue for Primary Cancer | blood |
Case history | Burkitt’s lymphoma | Metastasis | |
Tissue Metastasized | Genetics | ||
Life Span | infinite | Crisis PDL | |
Morphology | lymphocyte-like | Character | |
Classify | transformed | Established by | Kenzo Takada |
Registered by | BioVector NTCC Inc. | Regulation for Distribution | |
Comment | Year | 2017 | |
Medium | RPMI1640 medium with 800ug/ml G-418 and 10% heat inactivated fetal bovine serum | Methods for Passages | dilution |
Cell Number on Passage | Race | Japanese | |
CO2 Conc. | 5% | Tissue Sampling | blood |
Tissue Type |
Detection of virus genome fragment by Real-time PCR | |||||||||
---|---|---|---|---|---|---|---|---|---|
Detected DNA Virus | tested | Detected RNA Virus | tested | ||||||
CMV | - | parvoB19 | - | HCV | - | HTLV-1 | - | ||
EBV | + | HBV | - | HIV-1 | - | HTLV-2 | - | ||
HHV6 | - | HTLV-1 | - | HIV-2 | - | HAV | - | ||
HHV7 | - | HTLV-2 | - | -/negative. +/positive. nt/not tested. (positive (+) does not immediately mean the production of infectious viral particles.) |
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